Microbial production of polyhydroxybutyrate with tailor-made properties: An integrated modelling approach and experimental validation

Penloglou, Giannis; Chatzidoukas, Christos; Kiparissides, Costas

doi:10.1016/j.biotechadv.2011.06.021

Cited by 57 publications

(21 citation statements)

References 24 publications

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“…(a), a linear relationship between M w PHB and C/N ratio was observed in batch and one feeding‐pulse cultivations, independently of the stoichiometric and kinetic parameters achieved in both cultivation systems (Tables and ). In contrast, for a large number of microorganisms, it has been observed that high percentages of PHB accumulation are associated with low polymer M w however, in the OPNA the highest M w of PHB was achieved under conditions at which the strain even accumulated more than 80% of PHB. Although the feeding strategies are another parameter that could have an important effect on the PHB M w , only for the cultures conducted with C/N ratio of 18, the M w of PHB decreased 18.4% in the one feeding‐pulse and 52% in the two feeding‐pulse fed‐batch cultures with respect to the M w achieved in batch systems.…”

Section: Discussionmentioning

confidence: 88%

Production of polyhydroxybutyrate (PHB) of high and ultra-high molecular weight byAzotobacter vinelandiiin batch and fed-batch cultures

Castillo

Flores

Segura

et al. 2017

J. Chem. Technol. Biotechnol

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BACKGROUND Polyhydroxybutyrate (PHB) is a biopolymer with thermo‐mechanical properties like those of plastics produced by the petrochemical industry. These properties are improved in PHBs of high or ultra‐high weight average molecular weight. Azotobacter vinelandii OPNA is a potential producer of large quantities of PHB, having ultra‐high molecular weight by fed‐batch production strategies. RESULTS In batch and fed‐batch cultures at different carbon–nitrogen molar ratios (10, 14 and 18), the OPNA strain produced PHB of high and ultra‐high weight average molecular weight, with values between 2.3 and 6.6 MDa. In this strain, the weight average molecular weight was highly dependent on the initial carbon–nitrogen ratio, reaching the highest value (6.6 MDa) in batch cultures conducted with a carbon–nitrogen ratio of 18 and the lowest (2.3) with a carbon–nitrogen ratio of 10. On the other hand, using a two feeding‐pulses fed‐batch strategy, the highest global PHB volumetric productivity (0.56 g L−1 h−1) and polymer concentration (27.6 g L−1) were obtained. CONCLUSION The OPNA strain accumulated up to 80% of PHB of ultra‐high molecular weight as a function of the initial carbon–nitrogen molar ratio. Using a simple fed‐batch strategy of two feeding‐pulses was possible to produce up to 27 g L−1 of PHB. © 2016 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 88%

Production of polyhydroxybutyrate (PHB) of high and ultra-high molecular weight byAzotobacter vinelandiiin batch and fed-batch cultures

Castillo

Flores

Segura

et al. 2017

J. Chem. Technol. Biotechnol

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“…A mathematical model predicting biomass growth, substrate consumption, polymer production, as well as average molecular weight of P(3HB) under batch and fed-batch conditions, using Azohydromonas latus, was developed by Penloglou et al 87 Process optimization by model-designed fedbatch fermentation involving single feed of sucrose and ammonium sulphate was performed. It resulted in an accumulation of up to 95 % dcw polymer with a concentration of 11.84 g L -1 in 25 h. Batch mathematical model for P(3HB) production by Azohydromonas australica was developed by Gahlawat and Srivastava.…”

Section: Process Optimization Using Design-of-experiments Methodologymentioning

confidence: 99%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible intracellular polyesters that are accumulated as energy and carbon reserves by bacterial species, under nutrient limiting conditions. Successful large-scale production of PHAs is dependent on three crucial factors, which include the cost of substrate, downstream processing cost, and process development. In this respect, design and implementation of bioprocess strategies for efficient PHA bioconversions enable high PHA concentrations, yields and productivities. Additionally, development of PHA fermentation processes using inexpensive substrates, such as agro-industrial wastes, facilitates further cost reduction, thus benefitting large-scale PHA production. Thus, the aim of this review is to highlight various bioprocess strategies for high production of PHAs and their novel copolymers in relatively large quantities. This review also discusses the application of kinetic analysis and mathematical modelling as important tools for process optimization and thus improvement of the overall process economics for large-scale production of PHAs.

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“…A series of articles [104][105][106][107][108] published by Penloglou and coworkers, and Chadzidoukas et al was dedicated to the modeling of PHA production by Alcaligenes latus (today: Azohydromonas lata). A mathematical model that incorporates the metabolic regulation was used to predict the simultaneous bacterial growth and polymer accumulation, respecting the polymerization/kinetic mechanism for build-up of the polymer chains.…”

Section: Metabolic Models Targeted For Industrial Pha Biosynthesismentioning

confidence: 99%

Mathematical Modelling as a Tool for Optimized PHA Production

Novák

Koller

Braunegg

et al. 2015

Chem.Biochem.Eng.Q.

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c ARENA -Association for resource efficient and sustainable technologies, Inffeldgasse 21b, 8010 Graz, AustriaThe potential of poly(hydroxyalkanoates) (PHAs) to replace conventional plastic materials justifies the increasing attention they have drawn both at lab-scale and in industrial biotechnology.The improvement of large-scale productivity and biochemical/genetic properties of producing strains requires mathematical modeling and process/strain optimization procedures. Current models dealing with structurally diversified PHAs, both structured and unstructured, can be divided into formal kinetic, low-structured, dynamic, metabolic (high-structured), cybernetic, neural networks and hybrid models; these attempts are summarized in this review. Characteristic properties of specific groups of models are stressed in light of their benefit to the better understanding of PHA biosynthesis, and their applicability for enhanced productivity. Unfortunately, there is no single type of mathematical model that expresses exactly all the characteristics of producing strains and/or features of industrial-scale plants; in addition, the different requirements for modelling of PHA production by pure cultures or mixed microbial consortia have to be addressed. Therefore, it is crucial to sophisticatedly adapt and fine-tune the modelling approach accordingly to actual processes, as the case arises. For "standard microbial cultivations and everyday practices", formal kinetic models (for simple cases) and "low-structured" models will be appropriate and of great benefit. They are relatively simple and of low computational demand.To overcome the specific weaknesses of different established model types, some authors use hybrid models. Here, satisfying compromises can be achieved by combining mechanistic, cybernetic, and neural and computational fluid dynamics (CFD) models. Therefore, this hybrid modelling approach appears to constitute the most promising solution to generate a holistic picture of the entire PHA production process, encompassing all the benefits of the original modelling strategies. Complex growth media require a higher degree of model structuring. For scientific purposes and advanced development of industrial equipment in the future, real systems will be modelled by highly organized hybrid models.All solutions related to modelling PHA production are discussed in this review.

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Microbial production of polyhydroxybutyrate with tailor-made properties: An integrated modelling approach and experimental validation

Cited by 57 publications

References 24 publications

Production of polyhydroxybutyrate (PHB) of high and ultra-high molecular weight byAzotobacter vinelandiiin batch and fed-batch cultures

Production of polyhydroxybutyrate (PHB) of high and ultra-high molecular weight byAzotobacter vinelandiiin batch and fed-batch cultures

Strategies for Large-scale Production of Polyhydroxyalkanoates

Mathematical Modelling as a Tool for Optimized PHA Production

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