Microbial Production of Propionic Acid with Propionibacterium freudenreichii Using an Anion Exchanger-Based In Situ Product Recovery (ISPR) Process with Direct and Indirect Contact of Cells

Abstract

The recovery of an inhibiting product from a bioreactor soon after its formation is an important issue in industrial bioprocess development. In the present study, the potential of the anion exchanger-based in situ product recovery (ISPR) technique for the biocatalytic production of propionic acid was discussed. The focus of the current work was the selection of a suitable configuration of metabolically active cells for application in propionic acid production. Accumulation of propionic acid in fermentation broth caused feedback inhibition of the growth and biotransformation activity of Propionibacterium freudenreichii CICC 10019. Relevant product inhibition kinetics was discussed, and the results showed that keeping the aqueous propionic acid concentration below 10.02 g L⁻¹ was an essential prerequisite for ISPR process. A batch study, in which three ISPR configuration mode designs were compared, was conducted. The comparison indicated that employing an external direct mode had significant advantages over other modes in terms of increased productivity and product yield, with a corresponding decrease in the number of downstream processing steps, as well as in substrate consumption. The fed-batch culture using an external direct mode for the continuous accumulation of propionic acid resulted in a cumulative propionic acid concentration of 62.5 g L⁻¹, with a corresponding product yield of 0.78 g propionic acid/g glucose.

High Cell Density Culture of Dairy Propionibacterium sp. and Acidipropionibacterium sp.: A Review for Food Industry Applications

“…Above or below this range, the cell growth rate tends to decrease (as low as 0.06 h −1 under pH 5). Also, the lag phase is extended outside neutral conditions (Marshall & Odame-Darkwah, 1995 ; Seshadri & Mukhopadhyay, 1993 ; Wang et al, 2012b ; Zhuge et al, 2014 ). Gorret et al ( 2001 ) reported that optimal pH range for A. acidipropionici DSM 4900 biomass and EPS production was within pH 6.5 to 7, based on a response surface methodology (RSM).…”

“…The main bioprocess techniques applied to achieve high cell density culture (HCDC) of dairy PAB are the fed-batch bioreactor (Goswami & Srivastava, 2000 ; Ozadali et al, 1996 ), cell recycling (Crespo et al, 1991 ; Liu et al, 2016 ; Miyano et al, 2000 ), perfusion culture (Boyaval & Corre, 1987 ; Hatanaka et al, 1988 ; Nakano et al, 1996 ), extractive fermentation (Jin & Yang, 1998 ; Wang et al, 2012b , 2014 ), and cell immobilization bioreactor (Feng et al, 2011 ; Rickert et al, 1998 ; Wallenius et al, 2015 ).…”

“…Propionic acid inhibitory effects over cell cultures begin at concentration range of 5 to 10 g L −1 , the critical value being around 30 g L −1 , when cell growth is arrested (Suwannakham & Yang, 2005 ; Wang et al, 2012b ), and propionic acid formation is also disrupted due to inhibition of key enzymes propionyl CoA transferase, and oxaloacetate carboxyltransferase (Fig. 1 ) (Suwannakham & Yang, 2005 ).…”

Design and evaluation of a continuous semipartition bioreactor for in situ liquid‐liquid extractive fermentation

“…The former has utilized traditional modes of operation (batch, fed‐batch, and continuous) with other possibilities of repeated batch systems. These modes overtime have been implement in these configurations to improve yield, productivity, and product titer (Anvari & Khayati, 2009; Arora et al, 2007; Choi et al, 2013; Ezeji, Qureshi, & Blaschek, 2003; Thaddeus C. Ezeji, Qureshi, & Blaschek, 2007; Khayati, Ghaemi, Vasheghani‐farahani, & Pahlavanzadeh, 2012; Michiaki Matsumoto, Okuno, & Kondo, 2014; P. Wang, Wang, & Su, 2012; Wu et al, 2015).…”

Engineering Propionibacterium freudenreichii subsp. shermanii for enhanced propionic acid fermentation: Effects of overexpressing propionyl-CoA:Succinate CoA transferase

“…However, propionic acid fermentation usually suffers from low productivity, yield, and purity due to product inhibition and the co-production of acetic and succinic acids (Suwannakham et al, 2006). To date, extensive research efforts have focused on improving the fermentation process by, such as, cell immobilization (Liang et al, 2012;Suwannakham and Yang, 2005;Zhang and Yang, 2009;Zhu et al, 2012) and in-situ product removal (Jin and Yang, 1998;Wang et al, 2012), but very little has been done on metabolic engineering of propionibacteria (Ammar et al, 2013(Ammar et al, , 2014Suwannakham et al, 2006;Zhuge et al, 2013).…”