2020
DOI: 10.3390/antiox9111130
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Microbial Production of Retinyl Palmitate and Its Application as a Cosmeceutical

Abstract: Chemically synthesized retinyl palmitate has been widely used in the cosmetic and biotechnology industry. In this study, we aimed to demonstrate the microbial production of retinyl palmitate and the benefits of microbial retinyl palmitate in skin physiology. A heterologous retinyl palmitate biosynthesis pathway was reconstructed in metabolically engineered Escherichia coli using synthetic expression modules from Pantoea agglomerans, Salinibacter ruber, and Homo sapiens. High production of retinyl palmitate (69… Show more

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Cited by 13 publications
(18 citation statements)
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“…Collectively, the blhSR and raldhHS genes were functionally expressed in the BETA-1/pSTVM-blh-raldh strain, generating retinoic acid (0.52 ± 0.10 mg/L) from β-carotene. Notably, the presence of retinol (peak 2) and retinal (peak 3) in the extract of BETA-1/pSTVM2-blh was the same as that in our previous study [11].…”
Section: Construction Of Retinoic Acid Biosynthetic Pathway In the E Coli Beta-1 Strainsupporting
confidence: 80%
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“…Collectively, the blhSR and raldhHS genes were functionally expressed in the BETA-1/pSTVM-blh-raldh strain, generating retinoic acid (0.52 ± 0.10 mg/L) from β-carotene. Notably, the presence of retinol (peak 2) and retinal (peak 3) in the extract of BETA-1/pSTVM2-blh was the same as that in our previous study [11].…”
Section: Construction Of Retinoic Acid Biosynthetic Pathway In the E Coli Beta-1 Strainsupporting
confidence: 80%
“…The blh SR gene encoding β-carotene 15,15′-oxygenase (BlhSR) from S. ruber , which was previously cloned in the plasmid pUCMr-blh [ 11 ], was utilized, and the raldh HS gene encoding retinaldehyde dehydrogenase 2 (RALDH2, GenBank accession number, AB015226.1) from the Hep3B cell line was cloned into pUCMr to construct pUCMr-raldh ( Table 1 ). To construct the retinoic acid pathway, the two genes encoding BlhSR and RALDH2 were redesigned as an individual expression module, and then assembled into the plasmid pSTVM2, using the USER ® cloning method, resulting in pSTVM2-blh-raldh.…”
Section: Methodsmentioning
confidence: 99%
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“…In particular, the halophilic bacterium S . ruber has a retinal-based light-driven pump known as xanthorhodopsin [ 44 , 45 , 46 ]. Sesquiterpenoid and triterpenoid have commercial potential in the pharmaceutical sector due to their bioactivities such as antiviral, anticancer, anti-inflammatory, wound-healing properties, pigments, hormones and signaling molecules, antibiotics, antifeedants, or pollinator attractants.…”
Section: Resultsmentioning
confidence: 99%