Microbial production of succinic acid using crude and purified glycerol from a Crotalaria juncea based biorefinery

Sadhukhan, Suvra; Villa, Raffaella; Sarkar, Ujjaini

doi:10.1016/j.btre.2016.03.008

Cited by 40 publications

(23 citation statements)

References 34 publications

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“…As shown in Table 1, we found that crude glycerol inhibited glycerol consumption and succinate production. The previous study has also shown that crude glycerol inhibits the production of succinate by E. coli (Sadhukhan et al 2016). In this study, when the simply purified crude glycerol was used as carbon source, the inhibition of glycerol consumption and succinate production was significantly reduced compared with those using crude glycerol.…”

Section: Two-stage Fermentation For Succinate Production Using Differmentioning

confidence: 62%

“…To produce succinate from glycerol, different strategies of fermentation based on different engineered strains were applied, such as anaerobic (Zhang et al 2010), micro-aerobic (Blankschien et al 2010) and aerobic (Li et al 2013) cultures. Sadhukhan et al (2016) treated the crude glycerol through several physical-chemical steps: acidification, neutralization, solvent extraction, adsorption, and, finally, pressure filtration through a membrane. Then, the treated glycerol was used as carbon source to produce succinic acid by E. coli (ATCC 8739), but only 1.4 g/L succinic acid was accumulated.…”

Section: Introductionmentioning

confidence: 99%

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Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation

Huang

et al. 2018

Bioresour. Bioprocess.

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Background: Crude glycerol is a main by-product from biodiesel production, and efficient utilization of crude glycerol will bring significant economic and environmental benefits. However, the complex compositions of crude glycerol may impair the cellular growth and inhibit the crude glycerol consumption. Therefore, it is necessary to find a simple method to treat the crude glycerol and release the inhibition on cell metabolism. Results: The simply purified crude glycerol by activated carbon can be used as the carbon source to produce succinate in two-stage fermentation by the engineered Escherichia coli strain, MLB (ldhA − , pflB −) expressing phosphoenolpyruvate carboxykinase. In the flask experiments, succinate production from crude glycerol without treatment was less than that from pure glycerol. However, in the experiments of 1.5-L bioreactor, little succinate was produced in crude glycerol. The simply purified crude glycerol was used as carbon source for succinate production, and the glycerol consumption and succinate production were enhanced greatly. The succinate produced from the simply purified crude glycerol reached 566.0 mM, which was about ten times higher as that of non-purified one (50.3 mM). The succinate yield of the anaerobic stage achieved 0.97 mol/mol, which was 97% of the theoretical yield. Conclusion: The treatment of crude glycerol by activated carbon could effectively release the inhibition on the glycerol consumption and succinate production of the engineered E. coli strains, so that the fermentation result of the treated crude glycerol was similar as the pure glycerol. The results showed that the metabolically engineered E. coli strains have great potential to produce succinate from crude glycerol.

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Section: Two-stage Fermentation For Succinate Production Using Differmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation

Huang

et al. 2018

Bioresour. Bioprocess.

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“…The concentrations of succinic acid in the unknown solutions are estimated using standard curves prepared by plotting peak areas versus known concentrations of succinic acid samples. The entire process is optimized for a maximum production of succinic acid [26].…”

Section: Preparation Of Bio-refined Succinic Acid For Hydrogenationmentioning

confidence: 99%

“…First oil is extracted from the Crotalaria juncea seeds [23], which then gets trans-esterified using traditional and natural catalysts to produce a bio-diesel along with a huge quantity of waste glycerol [24]. The waste glycerol is then purified using sequential desalination [25] and further utilized as the primary substrate for producing bio succinic acid using E.Coli during microbial fermentation [26]. In this particular work, a number of Ru-Co bimetallic catalysts are synthesized with varying contents of Ru.…”

Section: Introductionmentioning

confidence: 99%

Liquid-phase hydrogenation of bio-refined succinic acid to 1,4-butanediol using bimetallic catalysts

et al. 2019

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Development of a Crotalaria juncea based biorefinery produce large quantity of waste glycerol after trans-esterification of the juncea seeds. This glycerol, after purification, is used as a substrate for producing succinic acid on a microbial route. Hydrogenation of this bio-refined succinic acid is carried out under high pressure in order to produce 1,4butanediol (BDO) using a batch slurry reactor with cobalt supported ruthenium bimetallic catalysts, synthesized inhouse. It is demonstrated that, using small amounts of ruthenium to cobalt increases the overall hydrogenation activity for the production of 1,4-butanediol. Hydrogenation reactions are carried out at various operating temperatures and pressures along with changes in the mixing ratios of ruthenium chloride and cobalt chloride hexahydrate, which are used to synthesize the catalyst. The Ru-Co bimetallic catalysts are characterized by XRD, FE-SEM and TGA. Concentrations of the hydrogenation product are analyzed using Gas chromatography-Mass spectrometry (GC-MS). Statistical analysis of the overall hydrogenation process is performed using a Box-Behnken Design (BBD).

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“…1,3-PD (Loh and Stasha, 2016;Jun et al, 2010), citric acid (Morgunov et al, 2013), succinic acid (Sadhukhan et al, 2016), ethanol (Chanthoom et al, 2016) and butanol (Jensen et al, 2012). Previously, several bacteria species had been identified for their ability to produce 1,3-PD such as Klebsiella (Wojtusik et al, 2015), Clostridium (Szymanowska-Powalowska, 2014), Enterobacter (Waghmare and Naik, 2016) and Citrobacter (Ferreira et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Fermentation of Biodiesel-Derived Waste for 1,3-Propanediol Production With Response Surface Methodology

Abel¹

2017

JOPR

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Microbial production of succinic acid using crude and purified glycerol from a Crotalaria juncea based biorefinery

Cited by 40 publications

References 34 publications

Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation

Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation

Liquid-phase hydrogenation of bio-refined succinic acid to 1,4-butanediol using bimetallic catalysts

Fermentation of Biodiesel-Derived Waste for 1,3-Propanediol Production With Response Surface Methodology

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