Microbial Production Potential of Pantoea ananatis: From Amino Acids to Secondary Metabolites

Usuda, Yoshihiro; Nishio, Yoshihiko; Nonaka, Gen; Hara, Yoshihiko

doi:10.3390/microorganisms10061133

Cited by 12 publications

(2 citation statements)

References 74 publications

(116 reference statements)

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“…Therefore, it is reasonable to suggest that some P. ananatis taxa associated with maize pollen may be commensals or possibly beneficial to host plants, following colonization of vegetative plant organs. Strains of P. ananatis have been shown to provide benefits to diverse host plants including growth promotion, ACC-deaminase activity, auxin production, nitrogen fixation, siderophore production, phosphorous solubilization, and biocontrol activities ( Sheibani-Tezerji et al, 2015 ; Usuda et al, 2022 ). Notably, P. ananatis was isolated from seeds of the ancient maize landrace Jala and shown to have nitrogen fixation and auxin production potential ( Johnston-Monje and Raizada, 2011 ); here, P. ananatis was shown to be present in Jala pollen.…”

Section: Discussionmentioning

confidence: 99%

Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq

Khalaf,

Shrestha,

Reid

et al. 2023

Front. Microbiol.

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Pollen is a vector for diversification, fitness-selection, and transmission of plant genetic material. The extent to which the pollen microbiome may contribute to host diversification is largely unknown, because pollen microbiome diversity within a plant species has not been reported, and studies have been limited to conventional short-read 16S rRNA gene sequencing (e.g., V4-MiSeq) which suffers from poor taxonomic resolution. Here we report the pollen microbiomes of 16 primitive and traditional accessions of maize (corn) selected by indigenous peoples across the Americas, along with the modern U.S. inbred B73. The maize pollen microbiome has not previously been reported. The pollen microbiomes were identified using full-length (FL) 16S rRNA gene PacBio SMRT sequencing compared to V4-MiSeq. The Pan-American maize pollen microbiome encompasses 765 taxa spanning 39 genera and 46 species, including known plant growth promoters, insect-obligates, plant pathogens, nitrogen-fixers and biocontrol agents. Eleven genera and 13 species composed the core microbiome. Of 765 taxa, 63% belonged to only four genera: 28% were Pantoea, 15% were Lactococcus, 11% were Pseudomonas, and 10% were Erwinia. Interestingly, of the 215 Pantoea taxa, 180 belonged to a single species, P. ananatis. Surprisingly, the diversity within P. ananatis ranged nearly 10-fold amongst the maize accessions analyzed (those with ≥3 replicates), despite being grown in a common field. The highest diversity within P. ananatis occurred in accessions that originated near the center of diversity of domesticated maize, with reduced diversity associated with the north–south migration of maize. This sub-species diversity was revealed by FL-PacBio but missed by V4-MiSeq. V4-MiSeq also mis-identified some dominant genera captured by FL-PacBio. The study, though limited to a single season and common field, provides initial evidence that pollen microbiomes reflect evolutionary and migratory relationships of their host plants.

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Section: Discussionmentioning

confidence: 99%

Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq

Khalaf,

Shrestha,

Reid

et al. 2023

Front. Microbiol.

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“…It is also noted for increasing crop yield of host plants as a result of promoting plant growth. [33].…”

Section: Discussionmentioning

confidence: 99%

Variations of the Bacterial Community in Wheat (Triticum aestivum), Oats (Avena sativa L.), Barley (Hordeum vulgare L.) and Triticale (Triticosecale) from Three Regions of the Republic of Crimea

Muvingi¹,

Slovareva²,

Yaremko³

et al. 2023

Preprint

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Russia is one of the largest cereal grain exporters in the world, churning out 34,3 million tons of export grain (wheat, barley and oats) in 2021. Plant infectious pathogens continue to be among the main factors in yield loss in the field and are a danger to the grain exporting industry's ability to expand internationally. This is primarily due to phytosanitary restrictions imposed by nations that monitor the presence and absence of certain phytopathogens in imported goods. Phytosanitary measures prevent the spread of plant pathogens, thus cutting the cost of dealing with them, once the pathogens invade new agricultural regions. This paper is devoted to the detection and identification of bacteria in samples of grain crops of three regions in The Republic of Crimea. The objects of the study were bacterial isolates from plant samples particularly wheat, oats, barley and triticale. The study was conducted in 2021. The identification of the isolates was carried out by sequencing a section 16–23S of the rRNA amplified by PCR with 8UA/519B, 27f/907r and PSf/PSr primers. Nucleotide sequences were deciphered using the Bio Edit program and compared with sequences placed in GenBank (https://blast.ncbi.nlm.nih. gov). The result of identification was considered an organism with maximum similarity. As a result, 38 samples of grain crops were collected, 95 bacterial colonies were isolated, of which 68 were identified to genus level and 22 were identified to species level. Some of the phytopathogens identified include: Agrococcus jenensis, Pseudomonas sp. and Curtobacterium sp. Some of the bacteria identified are beneficial like Ochrobactrum sp. Erwinia sp. and Pantoea sp. had a frequency of 28.95%, with Pantoea agglomerans having a frequency of 18.42%. Ochrobactrum sp. had a frequency of 10.53%. Enterococcus mundtii an frequency of 5.26%. Information about the species composition of bacteria on grain crops can be used to determine the spread of bacteria and their diagnosis and for bioinformatic analysis of genomes in search of species-specific genetic markers.

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Secondary Metabolites from Extremophiles

Mashakhetri,

Aishwarya,

Prusty

et al. 2024

Trends in Biotechnology of Polyextremophiles

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Microbial Production Potential of Pantoea ananatis: From Amino Acids to Secondary Metabolites

Cited by 12 publications

References 74 publications

Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq

Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq

Variations of the Bacterial Community in Wheat (<em>Triticum aestivum</em>), Oats (<em>Avena sativa</em> L.), Barley (<em>Hordeum vulgare</em> L.) and Triticale (<em>Triticosecale</em>) from Three Regions of the Republic of Crimea

Secondary Metabolites from Extremophiles

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