Microbial recognition of target-cell glycoconjugates

Karlsson, Karl‐Anders

doi:10.1016/0959-440x(95)80054-9

Cited by 314 publications

(169 citation statements)

References 72 publications

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“…Conversely, protein-carbohydrate interactions may also bring an untimely end to the organism's existence. Examples of this include the binding of some viral particles (influenza) and bacterial toxins (cholera toxin from Vibrio cholerae and pertussis toxin from Bordetella pertussis) to the plasma membrane of eukaryotic cells that is mediated by protein-carbohydrate interactions (microbial recognition of target-cell glycoconjugates has been reviewed in [5]). However, protein-protein interactions also provide fundamental triggers for a great number of inter-and intra-cellular signalling processes, perhaps the best characterized example being that of the growth hormone-growth hormone receptor complex [6].…”

Section: Introductionmentioning

confidence: 99%

The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro

Chapman

Kessopoulou

Andrews

et al. 1998

Biochemical Journal

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Human gamete interaction is of fundamental biological importance, yet the molecular interactions between spermatozoa and the zona pellucida are poorly understood. Surprisingly, the role of the polypeptide backbone of zona pellucida glycoprotein 3 (ZP3), the putative ligand for spermatozoa activation, has been largely overlooked. Purified recombinant human ZP3 was expressed in Escherichia coli as a C-terminal fusion to the dimeric glutathione S-transferase (GST) from Schistosoma japonicum and was shown to induce acrosomal exocytosis in live, capacitated human spermatozoa. The level of exocytosis is comparable with that obtained using purified, glycosylated, recombinant human ZP3 [van Duin, M., Polman, J. E. M., DeBreet, I. T. M., Van Ginneken, K., Bunschoten, H., Grootenhuis, A., Brindle, J. and Aitken, R. J. (1994). Biol Reprod. 51, 607-617]. These data imply that the polypeptide chain of human ZP3 contributes to recognition of spermatozoa during acrosomal exocytosis in vitro.

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Section: Introductionmentioning

confidence: 99%

The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro

Chapman

Kessopoulou

Andrews

et al. 1998

Biochemical Journal

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“…The length of the fatty acyl group may vary from C16 to C26 or more, and it may be modified by one or more hydroxyl groups and points of unsaturation. Gangliosides play important roles at the cell surface, serving as antigens and/or receptors, e.g., for adhesion [6,7], attachment of extracellular matrix components, viruses, bacteria and toxins [8,9], and as an important means for cell-cell communication [3,10]. The variations in their structures are especially important during nervous system development and carcinogenesis [2,[11][12][13].…”

mentioning

confidence: 99%

“…Like most glycoconjugates, gangliosides usually occur as mixtures of structural variants whose specific biological activity at any given time may be based on minor, transient components in the mixture. It is often not possible to obtain enough pure material for NMR structural determinations of the critical epitopes, and even when larger amounts of sample may be available, the progress of the isolation must be monitored by techniques with high specificity and sensitivity [2,9,14]. Due to its high sensitivity and capacity for dealing with complex mixtures, mass spectrometry often plays a critical role in the detection and structural determination of gangliosides.…”

mentioning

confidence: 99%

High pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for minimization of ganglioside fragmentation

O’Connor

Mirgorodskaya

Costello

2002

J. Am. Soc. Mass Spectrom.

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Transiently elevating pressure in a matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) source into the 1-10 mbar range during ionization decreases the metastable fragmentation of gangliosides. This allows detection of the molecular ion species without loss of the highly labile sialic acid residues. In these experiments, gangliosides with up to five sialic acids were ionized by MALDI and detected with the FTMS. In each case, when the high pressure collisional cooling was used, the singly charged molecular ion was the base peak in the spectra, both in the positive and negative ion modes, and minimal metastable fragmentation was observed. This result is promising, as the previously developed TLC separation methods can be coupled to MALDI-FTMS. (J Am Soc Mass Spectrom 2002, 13, 402-407)

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“…The carbohydrate structures that are linked to glycoproteins often function as attachment sites for bacteria and viruses (40,41). Recently, the carbohydrate structure sulfo-Le a was identified as an adhesion molecule for H. pylori (42,43).…”

Section: Introductionmentioning

confidence: 99%

Salivary MUC5B-Mediated Adherence (Ex Vivo) of Helicobacter pylori During Acute Stress

Bosch

Geus

Ligtenberg

et al. 2000

Psychosomatic Medicine

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Objective: Biochemical host defenses at mucosal sites, such as the oral cavity, play a key role in the regulation of microbial ecology and the prevention of infectious disease. These biochemical factors have distinct features, some of which benefit the host and some that benefit bacteria. We investigated the effects of acute stress on the salivary levels of the carbohydrate structure sulfo-Lewis a (sulfo-Le a ), which is linked to the mucosal glycoprotein MUC5B. Sulfo-Le a was recently identified as an adhesion molecule for Helicobacter pylori; therefore, we also measured saliva-mediated adherence (ex vivo) of H. pylori. The oral cavity is suspected to be involved in the transmission of H. pylori. Methods: Saliva was collected from 17 undergraduates before (baseline), during (stress), and after (recovery) exposure to a video showing surgical procedures. In addition, blood pressure, an impedance cardiogram, and an electrocardiogram were recorded. Results: During stressor exposure, participants reported increased state anxiety. In addition, stroke volume increased and heart rate decreased. The stressor induced a strong increase in salivary sulfo-Le a concentration (U/ml), sulfo-Le a output (U/min), sulfo-Le a /total protein ratio (U/mg protein), and saliva-mediated adherence (ex vivo) of H. pylori. As expected, sulfo-Le a concentration correlated with the adherence of H. pylori (r ϭ 0.72, p Ͻ .05). It was demonstrated that the observed adherence was induced by MUC5B and that the carbohydrate structure sulfo-Le a contributed to this process. Conclusions: Our study demonstrated a direct link between stress-mediated biochemical changes and altered host-microbe interactions in humans. Increased bacterial adherence may be a contributing factor in the observed relationship between stress and susceptibility to infectious disease.

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Microbial recognition of target-cell glycoconjugates

Cited by 314 publications

References 72 publications

The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro

The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro

High pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for minimization of ganglioside fragmentation

Salivary MUC5B-Mediated Adherence (Ex Vivo) of Helicobacter pylori During Acute Stress

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