Microbial Reduction of Fumonisin B1 by the New Isolate Serratia marcescens 329-2

Keawmanee, Pisut; Rattanakreetakul, Chainarong; Pongpisutta, R.

doi:10.3390/toxins13090638

Cited by 8 publications

(10 citation statements)

References 63 publications

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“…Results of these studies showed that the hydrolase and transferase enzymes, which can cooperate in the degradation of fumonisin, showed high expression compared to their levels in the control samples. These studies showed that Serratia marcescens 329-2 is a novel bacterium with the potential to reduce FB 1 , and the production of FB 1 -reducing enzymes should be further investigated [67].…”

Section: Novel Methodsmentioning

confidence: 99%

“…In terms of mycotoxin reduction, novel approaches have shown that fumonisin contamination can be reduced by detoxifying a microbial enzyme. In study [67], the bacterium Serratia marcescens 329-2 was identified from maize with high activity towards the reduction of FB 1 . A hydrolase and a transferase with increased expression in bacterial cells were also found, which may indicate a potential for the development of FB 1 -reducing enzymes [67].…”

Section: Novel Methodsmentioning

confidence: 99%

“…In study [67], the bacterium Serratia marcescens 329-2 was identified from maize with high activity towards the reduction of FB 1 . A hydrolase and a transferase with increased expression in bacterial cells were also found, which may indicate a potential for the development of FB 1 -reducing enzymes [67]. Results of these studies showed that the hydrolase and transferase enzymes, which can cooperate in the degradation of fumonisin, showed high expression compared to their levels in the control samples.…”

Section: Novel Methodsmentioning

confidence: 99%

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Mycotoxins—Prevention, Detection, Impact on Animal Health

Kępińska-Pacelik

Biel

2021

Processes

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Mycotoxins are defined as secondary metabolites of some species of mold fungi. They are present in many foods consumed by animals. Moreover, they most often contaminate products of plant and animal origin. Fungi of genera Fusarium, Aspergillus, and Penicillum are most often responsible for the production of mycotoxins. They release toxic compounds that, when properly accumulated, can affect many aspects of breeding, such as reproduction and immunity, as well as the overall liver detoxification performance of animals. Mycotoxins, which are chemical compounds, are extremely difficult to remove due to their natural resistance to mechanical, thermal, and chemical factors. Modern methods of analysis allow the detection of the presence of mycotoxins and determine the level of contamination with them, both in raw materials and in foods. Various food processes that can affect mycotoxins include cleaning, grinding, brewing, cooking, baking, frying, flaking, and extrusion. Most feeding processes have a variable effect on mycotoxins, with those that use high temperatures having the greatest influence. Unfortunately, all these processes significantly reduce mycotoxin amounts, but they do not completely eliminate them. This article presents the risks associated with the presence of mycotoxins in foods and the methods of their detection and prevention.

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Section: Novel Methodsmentioning

confidence: 99%

Section: Novel Methodsmentioning

confidence: 99%

Section: Novel Methodsmentioning

confidence: 99%

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Mycotoxins—Prevention, Detection, Impact on Animal Health

Kępińska-Pacelik

Biel

2021

Processes

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Section: Introductionmentioning

confidence: 99%

“…According to a recent study, the hydrolase and transferase enzymes of Serratia marcescens have shown the FB1 degradation (37%) efficiency. However, microbial methods may decrease the food quality by absorbing nutrients and emitting byproducts into the food matrices, and they are also more costly ( 18 ). These latter studies highlight the importance of careful characterization and evaluation of mycotoxin binders for use in specific mycotoxin contamination as well as appropriate in vivo models of target animal species to ensure efficacy and safety.…”

Section: Introductionmentioning

confidence: 99%

Protocatechuic acid: A novel detoxication agent of fumonisin B1 for poultry industry

Wang

Chen

et al. 2022

Front. Vet. Sci.

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Fumonisin B1 (FB1) is a major fusarium mycotoxin that largely contaminates feedstuffs and foods, posing a health risk to both animals and humans. This mycotoxin can enter the human body directly through contaminated food consumption or indirectly by toxins and their metabolites. In a prior study, feed-borne FB1 is one of the leading mycotoxins in breeder eggs, leading to reduced hatchability and gizzard ulceration in chicken progenies. Currently, no effective way is available to remove FB1 from feeds and human-contaminated foods. We hypothesize that FB1 can be reduced to low risk by protocatechuic acid (PCA). To assess the ability of FB1 to be degraded in vivo, 1 ppm of FB1 was treated with PCA, or D-glucose, or silymarin, or anti-FB1 monoclonal antibody. Our study revealed that both D-glucose and PCA exhibited 53.4 and 71.43% degradation, respectively, at 80°C for 2 h, while 35.15% of FB1 detoxification was determined in the silymarin group at 60°C for 0.5 h. A dose-dependent manner was found after treatment with D-glucose or PCA at 80°C for 2 h. As for detoxification of anti-FB1 monoclonal antibody, the 1:3,000 dilution induced significant FB1 detoxification, accounting for 25.9% degradation at 25°C for 2 h. Furthermore, 50 SPF 11-day-old embryonated eggs were divided into 10 groups, with five eggs per group. Post treatment with PCA or D-glucose, or silymarin or anti-FB1 monoclonal antibody, the treated samples were inoculated into albumens and monitored daily until the hatching day. Consequently, 100% of the chickens survived in the D-glucose group and other control groups, except for the FB1 control group, while 80, 80, and 60% hatching rates were found in the PCA-treated group, the anti-FB1 monoclonal antibody-treated group, and the silymarin-treated group. Additionally, both the FB1 group and the silymarin-treated group yielded lower embryo growth than other groups did. Postmortem, lower gizzard ulceration index was determined in the PCA-treated group and the anti-FB1 monoclonal antibody-treated group compared to those of the silymarin-treated group and D-glucose-treated group. Based on the above evidence, PCA is a promising detoxification to reduce FB1 contamination in the poultry industry, contributing to the eradication of mycotoxin residuals in the food chain and maintaining food security for human beings.

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