Microbial remediation of aromatics-contaminated soil

Xu, Ying; Zhou, Ning‐Yi

doi:10.1007/s11783-017-0894-x

Cited by 42 publications

(20 citation statements)

References 66 publications

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“…There is indeed a huge genetic potential in soil microorganisms to degrade key anthropogenic substances. Such (mainly aerobic) activities of microbes, in terms of pollution removal, have already been described from the 1980s onwards (Cheng et al 1983;Wilson and Jones 1993;Xu and Zhou 2017). Recent molecular approaches have confirmed most of these old data, and have even shown new important traits of the soil microbiome with respect to the degradation of pollutants.…”

Section: Soil Functions That Allow Filtering and Clean-up Of Percolatmentioning

confidence: 85%

Microbial indicators for soil quality

et al. 2017

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The living soil is instrumental to key life support functions (LSF) that safeguard life on Earth. The soil microbiome has a main role as a driver of these LSF. Current global developments, like anthropogenic threats to soil (e.g., via intensive agriculture) and climate change, pose a burden on soil functioning. Therefore, it is important to dispose of robust indicators that report on the nature of deleterious changes and thus soil quality. There has been a long debate on the best selection of biological indicators (bioindicators) that report on soil quality. Such indicators should ideally describe organisms with key functions in the system, or with key regulatory/connecting roles (so-called keystone species). However, in the light of the huge functional redundancy in most soil microbiomes, finding specific keystone markers is not a trivial task. The current rapid development of molecular (DNA-based) methods that facilitate deciphering microbiomes with respect to key functions will enable the development of improved criteria by which molecular information can be tuned to yield molecular markers of soil LSF. This review critically examines the current state-ofthe-art in molecular marker development and recommends avenues to come to improved future marker systems.

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Section: Soil Functions That Allow Filtering and Clean-up Of Percolatmentioning

confidence: 85%

Microbial indicators for soil quality

et al. 2017

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“…In the past decade, several physico-chemical methods have been reported on the degradation of 2C4NP (Priya and Madras 2006 ; Verma et al 2015 ); however, these methods are cost-consuming and do not yield complete mineralization of 2C4NP. Bioremediation process, which is more environmental-friendly and cost-effective in comparison with physico-chemical methods, has turned out to be a promising strategy for degradation of various nitrophenol pollutants (Chi et al 2013 ; Wang et al 2014 ; Min et al 2017a ; Xu and Zhou 2017 ). Moreover, microbial-based processes can be also effectively integrated with the activated sludge system which was already used in the conventional effluent treatment plants.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8

et al. 2018

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Cupriavidus sp. strain CNP-8 isolated from a pesticide-contaminated soil was able to utilize 2-chloro-4-nitrophenol (2C4NP) as a sole source of carbon, nitrogen and energy, together with the release of nitrite and chloride ions. It could degrade 2C4NP at temperatures from 20 to 40 °C and at pH values from 5 to 10, and degrade 2C4NP as high as 1.6 mM. Kinetics assay showed that biodegradation of 2C4NP followed Haldane substrate inhibition model, with the maximum specific growth rate (μmax) of 0.148/h, half saturation constant (Ks) of 0.022 mM and substrate inhibition constant (Ki) of 0.72 mM. Strain CNP-8 was proposed to degrade 2C4NP with hydroxyquinol (1,2,4-benzenetriol, BT) as the ring-cleavage substrate. The 2C4NP catabolic pathway in strain CNP-8 is significant from those reported in other Gram-negative 2C4NP utilizers. Enzymatic assay indicated that the monooxygenase initiating 2C4NP catabolism had different substrates specificity compared with previously reported 2C4NP monooxygenations. Capillary assays showed that strain CNP-8 exhibited metabolism-dependent chemotactic response toward 2C4NP at the optimum concentration of 0.5 mM with a maximum chemotaxis index of 37.5. Furthermore, microcosm studies demonstrated that strain CNP-8, especially the pre-induced cells, could remove 2C4NP rapidly from the 2C4NP-contaminated soil. Considering its adaptability to pH and temperature fluctuations and great degradation efficiency against 2C4NP, strain CNP-8 could be a promising candidate for the bioremediation of 2C4NP-contaminated sites.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0574-7) contains supplementary material, which is available to authorized users.

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“…Previous studies (Bhushan et al 2003 , 2004c , 2005a ) have shown that the degrading enzymes for nitromine chemicals are mostly NAD(P)H-dependent, as electron donation is required to transform the chemicals. For instance, nitroreductase, a member of the oxidoreductase enzyme family, can effectively degrade nitroaromatic compounds by transferring two electrons from NADPH to amines by reducing the nitro groups (Sree et al 2019 ; Xu and Zhou 2017 ). Interestingly, as shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20

Dang

Li³

et al. 2020

AMB Expr

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Hexanitrohexaazaisowurtzitane (CL-20) is a compound with a polycyclic cage and an N-nitro group that has been shown to play an unfavorable role in environmental fate, biosafety, and physical health. The aim of this study was to isolate the microbial community and to identify a single microbial strain that can degrade CL-20 with desirable efficiency. Metagenomic sequencing methods were performed to investigate the dynamic changes in the composition of the community diversity. The most varied genus among the microbial community was Pseudomonas, which increased from 1.46% to 44.63% during the period of incubation (MC0–MC4). Furthermore, the new strain was isolated and identified from the activated sludge by bacterial morphological and 16s rRNA sequencing analyses. The CL-20 concentrations decreased by 75.21 μg/mL and 74.02 μg/mL in 48 h by MC4 and Pseudomonas sp. ZyL-01, respectively. Moreover, ZyL-01 could decompose 98% CL-20 of the real effluent in 14 day’s incubation with the glucose as carbon source. Finally, a draft genome sequence was obtained to predict possible degrading enzymes involved in the biodegradation of CL-20. Specifically, 330 genes that are involved in energy production and conversion were annotated by Gene Ontology functional enrichment analysis, and some of these candidates may encode enzymes that are responsible for CL-20 degradation. In summary, our studies indicate that microbes might be a valuable biological resource for the treatment of environmental contamination caused by CL-20 and that Pseudomonas sp. ZyL-01 might be a promising candidate for eradicating CL-20 to achieve a more biosafe environment and improve public health.

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Microbial remediation of aromatics-contaminated soil

Cited by 42 publications

References 66 publications

Microbial indicators for soil quality

Microbial indicators for soil quality

Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8

Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20

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