2022
DOI: 10.1038/s42004-022-00711-6
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Microbial rhodoquinone biosynthesis proceeds via an atypical RquA-catalyzed amino transfer from S-adenosyl-L-methionine to ubiquinone

Abstract: Rhodoquinone (RQ) is a close analogue of ubiquinone (UQ) that confers diverse bacterial and eukaryotic taxa the ability to utilize fumarate as an electron acceptor in hypoxic conditions. The RquA protein, identified in a Rhodospirillum rubrum RQ-deficient mutant, has been shown to be required for RQ biosynthesis in bacteria. In this report, we demonstrate that RquA, homologous to SAM-dependent methyltransferases, is necessary and sufficient to catalyze RQ biosynthesis from UQ in vitro. Remarkably, we show that… Show more

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Cited by 2 publications
(3 citation statements)
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“…Indeed, isotope labelling experiments have suggested the oxidative decomposition of SAM to CO 2 , MTA and an aldehyde fragment during the transformation. 229 Mechanistic hypotheses for this transformation are shown in Fig. 32C and D; however, much work is still left to be done before the chemistry of this unique enzyme is fully understood.…”
Section: Enzymes With a Methyltransferase (Mt) Foldmentioning
confidence: 99%
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“…Indeed, isotope labelling experiments have suggested the oxidative decomposition of SAM to CO 2 , MTA and an aldehyde fragment during the transformation. 229 Mechanistic hypotheses for this transformation are shown in Fig. 32C and D; however, much work is still left to be done before the chemistry of this unique enzyme is fully understood.…”
Section: Enzymes With a Methyltransferase (Mt) Foldmentioning
confidence: 99%
“…227,228 RquA was recently characterized as a manganese-dependent enzyme that catalyzes transfer of the a-amino group from SAM to ubiquinone (160) to yield rhodoquinone (159). 229 While RquA resembles SAM-dependent methyltransferases, only a subset of the conserved residues in the predicted Rossmann fold generally associated with SAM binding are required for activity (Fig. 32B).…”
Section: Sam As An Amino Donormentioning
confidence: 99%
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