Microbial sensor cell arrays

Melamed, Sahar; Elad, Tal; Belkin, Shimshon

doi:10.1016/j.copbio.2011.11.024

Cited by 60 publications

(33 citation statements)

References 57 publications

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“…Several examples of detection of different classes of analytes, ranging from heavy metals to endocrine disruptors have been reported. 5,6 Nonetheless, the majority of previously reported devices lack adequate analytical performance for real-life applications, and have thus failed to reach the market. 7,8 To increase the robustness of such devices, several cell immobilization strategies have been investigated, aiming to keep the cells alive and responsive to the target analyte.…”

Section: Introductionmentioning

confidence: 99%

Bioengineered bioluminescent magnetotactic bacteria as a powerful tool for chip-based whole-cell biosensors

et al. 2013

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This paper describes the generation of genetically engineered bioluminescent magnetotactic bacteria (BL-MTB) and their integration into a microfluidic analytical device to create a portable toxicity detection system. Magnetospirillum gryphiswaldense strain MSR-1 was bioengineered to constitutively express a red-emitting click beetle luciferase whose bioluminescent signal is directly proportional to bacterial viability. The magnetic properties of these bacteria have been exploited as "natural actuators" to transfer the cells in the chip from the reaction to the detection area, optimizing the chip's analytical performance. A robust and cost-effective biosensor for the evaluation of sample toxicity, named MAGNETOX, based on lens-free contact imaging detection, has been developed. A microfluidic chip has been fabricated using multilayered black and transparent polydimethyl siloxane (PDMS) in which BL-MTB are incubated for 30 min with the sample, then moved by microfluidics, trapped, and concentrated in detection chambers by an array of neodymium-iron-boron magnets. The chip is placed in contact with a cooled CCD via a fiber optic taper to perform quantitative bioluminescence imaging after addition of luciferin substrate. A model toxic compound (dimethyl sulfoxide, DMSO) and a bile acid (taurochenodeoxycholic acid, TCDCA) were used to investigate the analytical performance of the MAGNETOX. Incubation with DMSO and TCDCA drastically reduces the bioluminescent signal in a dose-related manner. The generation of bacteria that are both magnetic and bioluminescent combines the advantages of easy 2D cell handling with ultra sensitive detection, offering undoubted potential to develop cell-based biosensors integrated into microfluidic chips.

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Section: Introductionmentioning

confidence: 99%

Bioengineered bioluminescent magnetotactic bacteria as a powerful tool for chip-based whole-cell biosensors

et al. 2013

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“…Consequently, MMC was adopted as a positive control to assess the number of histidine revertants colonies for TA102 [10,12,21]. There is no doubt that the biosensor yielded rapid results when compared with the Ames assay, and this is widely acknowledged [22,23]. An acknowledged limitation of biosensors is that future developments will require the characterisation and adoption of strong promoters offering genuine relevance with key target receptors [24].…”

Section: Discussionmentioning

confidence: 99%

“…This could be exclusively for a mutagenic sensor or for a wider suite of sensors for specific analytes and generic toxicity. Samples could be exposed to a suite of sensors simultaneously, allowing a real time response that could prove valuable for the protection of target receptors at a timescale commensurate with intervention [22]. The sensors could also be fabricated to permit compact analysis and the use of disposable electrodes.…”

Section: Discussionmentioning

confidence: 99%

Validation of SOS-lux Microbial Biosensors for Mutagenicity Assessment: Mitomycin-C as a Model Compound

Paton¹

2013

J Biosens Bioelectron

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Validation of SOS-lux Microbial Biosensors for Mutagenicity Assessment: Mitomycin-C as a Model Compound AbstractHuman health protection requires relating the bioaccessible concentration of a mutagen with the corresponding likely harm that could be caused through exposure. This requires designating the target receptor in need of protection, and a quantitative understanding of the likely pathways for mutagen availability. In this study, young children were selected as target receptors because of their tendency to directly ingest soils. Most data used to characterise a chemical mutagenicity has been extrapolated from rat-based assays using chemical ingestion or direct injection procedures. Mitomycin C was selected as a relevant model compound and extracted using an established in vitro digestion technique. A range of mutagenic bioassays (i.e. SOS-lux based microbial biosensors and Salmonella mutagenicity assay) were calibrated and optimised in aqueous samples, before being applied to soil extracts. The biosensors were consistently as sensitive and responsive as the traditional Salmonella assay, however, the use of microbial biosensors offered speed and ease of analysis. The data presented confirm that the in vitro digestion bioassay enabled a rapid and inexpensive technique for deriving critical values for the protection of humans exposed to soil borne mutagenic pollutants.

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“…1 The micro-scale cell patterning can be applied not only in the field of tissue engineering but also in cell-cell interaction, 2 drug screening, 3 and biosensors. 4,5 Some methods for cell patterning that have been proposed mainly use forces to manipulate the cells, such as using the optical tweezers to control a single cell 6 or using a microlens array as an optical tweezers array to manipulate multiple cells; 7 the cells can also be patterned via acoustic tweezers based on the standing wave of sound; 8 other methods include dielectrophoretic force, 9,10 magnetic micropillar array, 11 micro magnetic concentrator devices, 12 or magnetic thin film array. 13 These active methods are used mostly for the patterning of suspension cells.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of hexagonally packed cell culture substrates using droplet formation in a T-shaped microfluidic junction

2013

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A method is here proposed to fabricate ordered hexagonally packed cell culture substrates with hexagonally arranged cell patterning areas. We generated photo-sensitive polymeric microdroplets in a T-shaped microfluidic junction by an immiscible liquid, and then solidified the collective self-assembled hexagonal droplet array to obtain the cell culture substrate, on which we took the grooves formed between the solidified droplets as the hexagonally arranged cell patterning areas. The most promising advantage of our method is that we can actively tune the droplet size by simply adopting different volumetric flow rates of the two immiscible fluids to form cell culture substrates with differently sized cell patterning areas. Besides, the examination results of the cell culture substrate's characteristics validate whether our method is capable of creating substrates with high spatial uniformity. To verify the cell patterning function of our cell culture substrates, we used the semi-adherent RAW cells to demonstrate the effectiveness of patterning of suspended/adherent cells before/after adhesion. Over 90% cell viability and cell patterning rate suggest that our method may be a promising approach for future applications of cell patterning on biochips. V C 2013 American Institute of Physics. [http://dx

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Microbial sensor cell arrays

Cited by 60 publications

References 57 publications

Bioengineered bioluminescent magnetotactic bacteria as a powerful tool for chip-based whole-cell biosensors

Bioengineered bioluminescent magnetotactic bacteria as a powerful tool for chip-based whole-cell biosensors

Validation of SOS-lux Microbial Biosensors for Mutagenicity Assessment: Mitomycin-C as a Model Compound

Fabrication of hexagonally packed cell culture substrates using droplet formation in a T-shaped microfluidic junction

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