Microbial Strain Engineering

Sanghavi, Gaurav; Gupta, Prabuddha; Rajput, Mahendrapalsingh; Oza, Tejas; Trivedi, Ujwalkumar; Singh, Nitin Kumar

doi:10.1007/978-981-15-2604-6_2

Cited by 6 publications

(1 citation statement)

References 69 publications

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“…Random mutagenesis is universally used to induce genetically improved strains. This is a cost-effective method for obtaining an improved strain for industrial purposes (Sanghavi et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. Isolated from Vietnamese Traditional Fermented Soybean (Tuong ban) using Ultraviolet Irradiation and Chemical Mutation

Thanh¹,

Hang²,

Anh³

et al. 2022

Int.J.Curr.Microbiol.App.Sci

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Forty-eight out of sixty-eight bacterial strains isolated from traditional fermented soybean samples (Tuong Ban) showed moderate to high fibrinolytic enzyme activity. Bacillus sp. HY6 had the highest fibrinolytic enzyme activity of 80.4 ± 1.2 FU/mL. The strain was selected for improving fibrinolytic activity via UV irradiation and chemically induced mutagenesis using Ethidium Bromide (EtBr) and Ethyl Methane Sulfonate (EMS). After UV-irradiation, the HY6-derived mutant, designated as strain U5_90.5, exhibited the highest fibrinolytic enzyme activity of 127 ± 5 FU/mL, about 1.6 times higher than that of the wild type HY6 strain. Strain U5_90.5 after UV mutation was subsequently subject to chemically induced mutation by EtBr and EMS. This double mutation resulted in strain ES4 which has the highest fibrinolytic enzyme activity of 404 ± 4 FU/mL. The enzyme production capability of the strain was stable after 10 generations which renders the strain a potential fibrinolytic enzyme producing strain for commercial applications. This result indicated that the Bacillus sp. ES4 could be a potential commercialized fibrinolytic enzyme producing bacterium.

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Section: Introductionmentioning

confidence: 99%