Microbial synthesis of saccharides in soils incubated with 13C-labelled glucose

Murayama, Shigetoshi

doi:10.1016/0038-0717(88)90036-3

Cited by 21 publications

(24 citation statements)

References 21 publications

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“…1). This trend is in agreement with previous studies (Murayama, 1988;Derrien et al, 2007) and illustrates the almost complete consumption of the substrate in a few days. Approximately 30 to 50 % of the consumed material was converted into microbial products, and the remaining part was used for heterotrophic respiration.…”

Section: Comparison Of the Four Substrates Mineralizationsupporting

confidence: 93%

“…Paul et al: Hydrogen dynamics in soil organic matter derived more from microbially derived products than from plant-derived molecules as a result of the mineral protection processes (Derrien et al, 2007;Bol et al, 2009). Carbon dynamics in this continuum have been widely studied using the natural 14 C / 12 C and 13 C / 12 C (Rafter and Stout, 1970;Balesdent et al, 1987) ratios and also through labeling experiments (Jenkinson, 1965;Murayama, 1988;Derrien et al, 2004). The results of these experiments have highlighted the different turnover of soil organic pools.…”

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confidence: 99%

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Hydrogen dynamics in soil organic matter as determined by 13C and 2H labeling experiments

et al. 2016

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Abstract. Understanding hydrogen dynamics in soil organic matter is important to predict the fate of 3 H in terrestrial environments. One way to determine hydrogen fate and to point out processes is to examine the isotopic signature of the element in soil. However, the non-exchangeable hydrogen isotopic signal in soil is complex and depends on the fate of organic compounds and microbial biosyntheses that incorporate water-derived hydrogen. To decipher this complex system and to understand the close link between hydrogen and carbon cycles, we followed labeled hydrogen and labeled carbon throughout near-natural soil incubations. We performed incubation experiments with three labeling conditions: 1 -13 C 2 H double-labeled molecules in the presence of 1 H 2 O; 2 -13 C-labeled molecules in the presence of 2 H 2 O; 3 -no molecule addition in the presence of 2 H 2 O. The preservation of substrate-derived hydrogen after 1 year of incubation (ca. 5 % in most cases) was lower than the preservation of substrate-derived carbon (30 % in average). We highlighted that 70 % of the C-H bonds are broken during the degradation of the molecule, which permits the exchange with water hydrogen. Added molecules are used more for trophic resources. The isotopic composition of the non-exchangeable hydrogen was mainly driven by the incorporation of water hydrogen during microbial biosynthesis. It is linearly correlated with the amount of carbon that is degraded in the soil. The quantitative incorporation of water hydrogen in bulk material and lipids demonstrates that nonexchangeable hydrogen exists in both organic and mineralbound forms. The proportion of the latter depends on soil type and minerals. This experiment quantified the processes affecting the isotopic composition of non-exchangeable hydrogen, and the results can be used to predict the fate of tritium in the ecosystem or the water deuterium signature in organic matter.

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Section: Comparison Of the Four Substrates Mineralizationsupporting

confidence: 93%

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confidence: 99%

Hydrogen dynamics in soil organic matter as determined by 13C and 2H labeling experiments

et al. 2016

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“…The soil samples (10 g) were placed in a serum bottle (160 ml) and mixed with 50 mg C 3 sugar (δ 13 C = -26.0‰) and 0.17 mg NH 4 NO 3 as four replicates and before the serum bottles were sealed with rubber septa all the samples were brought to 55% of water holding capacity (Murayama 1988). Moreover, the same amount of soil samples was prepared as a control with the same treatment except the sugar addition.…”

Section: Incubation Experiments and Analysesmentioning

confidence: 99%

Contribution of soil organic carbon and C3 sugar to the total CO2 efflux using 13C abundance

Koçyiğit¹

2006

PLANT SOIL ENVIRON

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The differences in C isotope ratio of C 3 and C 4 plant species have been used to determine relative contributions of carbon (C) sources to total CO 2 efflux. The objective of this study was to estimate the contribution of soil organic C and C 3 sugar to total CO 2 of corn and wheat monocultures during a short-term incubation. Control soils and soils amended with sugar were incubated at 25°C for 48 hours and total CO 2 concentration and δ 13 C values of evolved CO 2 were measured. The proportional contribution of C sources on CO 2 efflux was determined by using isotopic composition of soil organic C and C 3 sugar. δ 13 C values of soils are highly affected by the type of vegetation and the soil management. The C 3 sugar addition in soils double the CO 2 efflux in the corn soil, but it did not affect CO 2 efflux in the wheat soil. This indicated a larger turnover of microbial biomass in the corn soil. The greatest significant (P < 0.05) difference in δ 13 C values between the control and sugar added soils occurred at 12 hours in the corn soil (11.2‰) and at 24 hours in the wheat soil (9.4‰). The estimated relative contribution of sugar to CO 2 efflux was stronger at 12 hours incubation in the corn soil.

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“…1). The higher proportion of cellulosic glucose and xylose suggests that most of the saccharides consisting of original plant carbohydrates still remained, as the microbial synthesis in the soil of these components was considerably less active than that of other components (Cheshire 1979;Murayama 1988).…”

Section: R E S U L T Smentioning

confidence: 99%

“…A large difference between non-cellulosic and cellulosic saccharides in the remaining proportion was also noticeable. The microbial synthesis of non-cellulosic saccharides such as non-cellulosic glucose, galactose, mannose, ribose, and fucose (Cheshire 1979;Murayama 1988) may account for the greater remaining proportion of non-cellulosic saccharides. Actually, the remaining proportions of these components were greater than those of cellulosic glucose (Fig.…”

Section: Decomposition Of Constituentsmentioning

confidence: 99%

Chemical properties of subsurface peats and their decomposition kinetics under field conditions

Murayama

Asakawa

Ohno

1990

Soil Science and Plant Nutrition

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Microbial synthesis of saccharides in soils incubated with 13C-labelled glucose

Cited by 21 publications

References 21 publications

Hydrogen dynamics in soil organic matter as determined by <sup>13</sup>C and <sup>2</sup>H labeling experiments

Hydrogen dynamics in soil organic matter as determined by <sup>13</sup>C and <sup>2</sup>H labeling experiments

Contribution of soil organic carbon and C<sub>3</sub> sugar to the total CO<sub>2</sub> efflux using <sup>13</sup>C abundance

Chemical properties of subsurface peats and their decomposition kinetics under field conditions

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Microbial synthesis of saccharides in soils incubated with 13C-labelled glucose

Cited by 21 publications

References 21 publications

Hydrogen dynamics in soil organic matter as determined by &lt;sup&gt;13&lt;/sup&gt;C and &lt;sup&gt;2&lt;/sup&gt;H labeling experiments

Hydrogen dynamics in soil organic matter as determined by &lt;sup&gt;13&lt;/sup&gt;C and &lt;sup&gt;2&lt;/sup&gt;H labeling experiments

Contribution of soil organic carbon and C<sub>3</sub> sugar to the total CO<sub>2</sub> efflux using <sup>13</sup>C abundance

Chemical properties of subsurface peats and their decomposition kinetics under field conditions

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Hydrogen dynamics in soil organic matter as determined by <sup>13</sup>C and <sup>2</sup>H labeling experiments

Hydrogen dynamics in soil organic matter as determined by <sup>13</sup>C and <sup>2</sup>H labeling experiments