Microbial volatile organic compounds as indicators of fungi. Can an electronic nose detect fungi in indoor environments?

Kuske, Martyna; Romain, Anne‐Claude; Nicolas, Jacques

doi:10.1016/j.buildenv.2004.08.012

Cited by 110 publications

(68 citation statements)

References 46 publications

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“…These volatile metabolites are produced during both primary and secondary metabolism and are often collectively referred to as microbial volatile organic compounds (MVOCs) [3]. MVOCs are widely investigated as the indicator of fungal growth [4][5][6][7], mycotoxins' production [8][9][10] and for fungal taxonomy [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites

et al. 2016

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Aspergillus flavus produces dangerous secondary metabolites known as aflatoxins, which are toxic and carcinogenic, and their contamination of agricultural products results in health issues and economic hardships in the U.S. and around the world. Early identification of aflatoxigenic isolates of A. flavus is the key in the management of these fungi. An emerging detection method for specific fungi identification involves the analysis of microbial volatile organic compounds (MVOCs) released by the fungi. Complicating this approach is the understanding that many factors influence metabolic production, including growth parameters, such as growth media, temperature, spore counts and oxidation stress. In addition, analytical and data analysis methods can also influence the results. Several growth and analysis methods were evaluated and optimized in order to better understand the effect of the methods on fungi MVOC signatures. The results indicate that carboxen/polydimethylsiloxane (CAR/PDMS) has the best extraction efficiency for the MVOCs emitted by A. flavus. Both chemical defined agar (CDA) and chemical defined liquid (CDL) are suitable growth media for MVOC emission studies. The highest MVOC production was found at 30˝C. Log transformation was considered one of the best data pretreatment methods when analyzing MVOC data and resulted in the best principal component analysis (PCA) clustering in the experiments with different growth media. This study aims to elucidate fungal volatile organic compounds (VOCs) differences due to variations in growth parameters as a first step in the development of an analytical method for the monitoring of aflatoxigenic A. flavus contamination in crop storage facilities.

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Section: Introductionmentioning

confidence: 99%

Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites

et al. 2016

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“…Other kind of technique is based on the MVOCs detection by sensor-based devices, e.g. polymer sensors responsive to variation in electrical conductivity due to VOC adsorption (Joblin et al, 2010) or electron noses (e-noses), based on rapid detection and identification of a preselected range of volatile compounds (Kuske et al, 2005;Pinzari et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Detection of volatile metabolites of moulds isolated from a contaminated library

Micheluz

Manente

Rovea

et al. 2016

Journal of Microbiological Methods

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The principal fungal species isolated from a contaminated library environment were tested for their microbial volatile organic compound (MVOC) production ability. Aspergillus creber, A. penicillioides, Cladosporium cladosporioides, Eurotium chevalieri, E. halophilicum, Penicillium brevicompactum and P. chrysogenum were cultivated on suitable culture media inside sample bottles specifically designed and created for direct MVOC injection to a GC-MS instrument. The fungal emissions were monitored over several weeks to detect changes with the aging of the colonies, monitored also by respirometric tests. A total of 55 different MVOCs were detected and isopropyl alcohol, 3-methyl-1-butanol and 2-butanone were the principal compounds in common between the selected fungal species. Moreover, 2,4-dimethylheptane, 1,4-pentadiene, styrene, ethanol, 2-methyl-1-butanol, acetone, furan and 2-methylfuran were the most detected compounds. For the first time, the MVOC production for particular fungal species was detected. The species A. creber, which belongs to the recently revised group Aspergillus section Versicolores, was characterized by the production of ethanol, furan and 1,4-pentadiene. For the xerophilic fungus E. halophilicum, specific production of acetone, 2-butanone and 1,4-pentadiene was detected, supported also by respirometric data. The results demonstrated the potential use of this method for the detection of fungal contamination phenomena inside Cultural Heritage's preservation environments.

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“…1). These compounds belong to different groups of organic compounds [22,25,32,43]: hydrocarbons (heptane, isoprene), aromatic hydrocarbons (benzene, styrene), alcohols (1-octen-3-ol, 3-octanol, 3-methyl-1-butanol), aldehydes, ketones (1-octanone, 1-octen-3-on, 3-octanone), organic acids, ethers, esters (butyl acetate), terpenes (limonene), sesquiterpenes (humulene, nerolidol, bisabolene), organic compounds containing sulphur and nitrogen. Qualitative analysis was focused only on the compounds that could have been identified with probability higher than 90%.…”

Section: Qualitative Analysismentioning

confidence: 99%

Application of solid phase microextraction–gas chromatography–mass spectrometry method for the detection of active moulds on historical objects

Sawoszczuk

Syguła‐Cholewińska

2017

Herit Sci

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The main purpose of these studies was to assess the possibility of applying the technique of solid phase microextraction (SPME)-gas chromatography (GC)-mass spectrometry (MS) to detect the activity of moulds on historical objects, based on the analysis of microbial volatile organic compounds (MVOCs). The studies were performed for selected species of moulds, which were inoculated onto model samples of silk, cellulose, parchment and wool that had been prepared on microbiological medium, in vials for headspace sampling. After a few days of incubation, the MVOCs in the vials were sampled by using SPME fibre, and then they were analysed in the GC-MS system. The acquired chromatograms were qualitatively and quantitatively assessed, and it was ascertained that among the identified compounds are markers of mould activity which can be used to detect the vital mould growing on actual historic items. This usefulness of the method was additionally confirmed by analysis of MVOCs emitted by keratinolytically active mould inoculated on a sample of historical wool prepared in a Petri dish without a medium.

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Microbial volatile organic compounds as indicators of fungi. Can an electronic nose detect fungi in indoor environments?

Cited by 110 publications

References 46 publications

Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites

Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites

Detection of volatile metabolites of moulds isolated from a contaminated library

Application of solid phase microextraction–gas chromatography–mass spectrometry method for the detection of active moulds on historical objects

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