Microbial β-glucanases: production, properties, and engineering

Characterization of a novel cold-adapted GH1 β-glucosidase from Psychrobacillus glaciei and its application in the hydrolysis of soybean isoflavone glycosides

“…This process has been investigated by directly using of β-glucosidases from leguminous plants and microorganisms ( Kaya et al, 2008 ; Cao et al, 2018 ; Li et al, 2018 ; Abdella et al, 2018 ). However, in the industrial production of isoflavones, the low solubility of isoflavones and the poor stability of β-glucosidase, product inhibition, limited enzymatic hydrolysis efficiency, and other scientific problems reduce the conversion efficiency of isoflavone aglycones ( Godse et al, 2021 ; Ouyang et al, 2023 ; Jin et al, 2023 ). Therefore, the hydrolysis rate of soybean isoflavones by recombinant β-glucosidase PgBgl1 was determined using soybean isoflavone glycosides as substrates.…”