Microbially Induced Calcite Precipitation Employing Environmental Isolates

Abstract

In this study, five microbes were employed to precipitate calcite in cohesionless soils. Four microbes were selected from calcite-precipitating microbes isolated from calcareous sand and limestone cave soils, with Sporosarcina pasteurii ATCC 11859 (standard strain) used as a control. Urease activities of the four microbes were higher than that of S. pasteurii. The microbes and urea–CaCl2 medium were injected at least four times into cohesionless soils of two different relative densities (60% and 80%), and the amount of calcite precipitation was measured. It was found that the relative density of cohesionless soils significantly affects the amount of calcite precipitation and that there is a weak correlation between urease activity and calcite precipitation.

In Situ Cultured Bacterial Diversity from Iron Curtain Cave, Chilliwack, British Columbia, Canada

“…Although most of the other studies identified Sporosarcina as rare Firmicutes in the cave environment [38], our study identified 46.15% of Sporosarcina. In agreement with our study, a study by Kim et al [38] has revealed two bacterial isolates, Sporosarcina globispora and Sporosarcina spp., from limestone cave soil samples that play crucial roles in calcite precipitation in the cave soil. Bacillus (15.38%) has also been observed as one of the genus in our study.…”

Isolation, differentiation and biodiversity of ureolytic bacteria of Qatari soil and their potential in microbially induced calcite precipitation (MICP) for soil stabilization

“…B. cereus isolated from soil and marine environment are known to hydrolyse urea. 9,17,27,28 Similarly, B. licheniformis is also involved to precipitate calcium carbonate by ureolysis. 16 All the species that tested positive for ureolytic activity could grow on urea as sole carbon and nitrogen source consistently.…”

“…15 Bacillus is the most common genus of bacteria known to perform biomineralization by implementing ureolytic activities. Some specic species are B. sphaericus, 7 B. lentus, 17 B. pasteurii 30 and B. licheniformis. 16 Bacteria involved in biomineralization are important in stabilizing soil, especially in regions and cities with windy and desert-like conditions such as in the Gulf region.…”

Plasticity characteristics of lateritic soil treated with Sporosarcina pasteurii in microbial-induced calcite precipitation application

“…The cementation reagent is composed of 20 g urea, 10 g NH 4 Cl, 3 g nutrient broth, 2.8 g CaCl 2 and 2.12 g NaHCO 3 per liter of de-ionized water reported in several studies (e.g., [4,12,23,38,63,69,71,72]. In all the studies cited, 3 g/l of nutrient broth was added to the cementation reagent because it was the most viable amount for survival of bacteria [66].…”