Microbiologic and virulence characteristics ofMoraxella catarrhalisisolates from Zambian children presenting with acute pneumonia

Abstract

Background: Moraxella catarrhalis is one of the bacterial pathogens associated with childhood pneumonia, but its clinical importance is not clearly defined. Objective: This study aimed to investigate the microbiologic and virulence characteristics of M. catarrhalis isolates obtained from children with pneumonia in Lusaka, Zambia. Methods: This retrospective, cross-sectional study analyzed 91 M. catarrhalis isolates from induced sputum samples of children less than 5 years of age with pneumonia enrolled in the Pneumonia Etiology Research for Child Health study in Lusaka, Zambia between 2011 and 2014. Bacteria identification and virulence genes detection were performed by PCR and DNA sequencing, while antimicrobial susceptibility testing was determined by the Kirby-Bauer method.Results: All the M. catarrhalis isolates were obtained from good-quality sputum samples and were the predominant bacteria. These isolates harbored virulence genes copB (100%), ompE (69.2%), ompCD (71.4%), uspA1 (92.3%), and uspA2 (69.2%) and were all β-lactamase producers. They showed resistance to ampicillin (100%), amoxicillin (100%), trimethoprim-sulfamethoxazole (92.3%), ciprofloxacin (46.2%), chloramphenicol (45.1%), erythromycin (36.3%), tetracycline (25.3%), cefuroxime (11.0%), and amoxicillin-clavulanate (2.2%), with 71.4% displaying multi-drug resistant phenotype but all susceptible to imipenem (100%). Conclusion:This study showed that M. catarrhalis isolates were the predominant or only bacterial isolates from the sputum samples analyzed. The findings provide supportive evidence for the pathogenic potential role of this bacterium in pediatric

Isolation and Antimicrobial Resistance Patterns of Bacterial Pathogens from Community‐Acquired Pneumonia at Adama Hospital Medical College, Adama, Ethiopia

“…However, the findings of this study was in variation with the findings conducted in Jimma town, Southwestern Ethiopia [ 28 ] where K. pneumonia and P. aeruginosa were the most frequent isolates followed by other species. The predominant M. catarrhalis in this finding showed the significant emergency of this pathogen as an etiology of pneumonia in recent years [ 36 ]. The prevalence of S. aureus in CAP has also increased in recent decades, with high emergence of new lineages of MRSA in the community [ 37 ].…”

The spectrum of bacterial infectionin a large cohort of Chinese pediatric patients with inborn errors of immunity: a nine-year, retrospective, single-center study

“…β-lactamase production 68.63% (35/51) 42.9% [23] M. catarrhalis β-lactamase production 100% (26/26) 60.5% [23] , 100% [24] S. pneumoniae PNSP 4.35% (1/23) 39.4% [25] , 43.1% [26] S. aureus MRSA 55.17% (16/29) 34.5% [15] , 38.1 [27] K. pneumoniae EBSL 78.13% (25/32) 6.1% [23] K. pneumoniae CRKP 25.71% (9/35) 19.3% [15] E.coli EBSL 71.43% (15/21) 19.1% [23] E.coli CREC 38.10% (8/21) 16.3% [28] P. aeruginosa CRPA 25.81% (8/31) 12.8% [15] A. baumannii CRAB 70.59% (12/17) 45.4% [15] PNSP: Penicillin non-susceptible pneumococci (non-CNS).…”

“…Common drug-resistant bacteria in children with IEI have high AMR. [15,[23][24][25][26][27][28][29] This study summarized AMR of common drug-resistant bacteria in IEI patients (Table 3). For H. influenzae and M. catarrhalis, the β-lactamase production rates were 68.63 % and 100 %, respectively.…”