Microbiological activity of biotin-vitamers.

Ohsugi, Masahiro; Imanishi, Yasuko

doi:10.3177/jnsv.31.563

Cited by 21 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although such a CoA ligase (BioW) was identified in the gram‐positive bacterium Bacillus subtilis (Manandhar & Cronan, ), S. cerevisiae Bio1 protein does not show similarity to that enzyme. Additionally, biosynthesis of pimelic acid by S. cerevisiae has not been reported, and pimelic acid feeding to a strain carrying the full biotin biosynthesis pathway was not able to stimulate growth on medium lacking biotin (Ohsugi & Imanishi, ).…”

Section: Vitamins That Act As Enzyme Cofactorsmentioning

confidence: 99%

Vitamin requirements and biosynthesis in Saccharomyces cerevisiae

Perli

Wronska

Ortiz‐Merino

et al. 2020

Yeast

View full text Add to dashboard Cite

Chemically defined media for yeast cultivation (CDMY) were developed to support fast growth, experimental reproducibility, and quantitative analysis of growth rates and biomass yields. In addition to mineral salts and a carbon substrate, popular CDMYs contain seven to nine B‐group vitamins, which are either enzyme cofactors or precursors for their synthesis. Despite the widespread use of CDMY in fundamental and applied yeast research, the relation of their design and composition to the actual vitamin requirements of yeasts has not been subjected to critical review since their first development in the 1940s. Vitamins are formally defined as essential organic molecules that cannot be synthesized by an organism. In yeast physiology, use of the term “vitamin” is primarily based on essentiality for humans, but the genome of the Saccharomyces cerevisiae reference strain S288C harbours most of the structural genes required for synthesis of the vitamins included in popular CDMY. Here, we review the biochemistry and genetics of the biosynthesis of these compounds by S. cerevisiae and, based on a comparative genomics analysis, assess the diversity within the Saccharomyces genus with respect to vitamin prototrophy.

show abstract

Section: Vitamins That Act As Enzyme Cofactorsmentioning

confidence: 99%

Vitamin requirements and biosynthesis in Saccharomyces cerevisiae

Perli

Wronska

Ortiz‐Merino

et al. 2020

Yeast

View full text Add to dashboard Cite

show abstract

“…S288C (19), a typical laboratory strain of Saccharomyces cerevisiae, is an example of such organisms. It is auxotrophic for biotin and requires biotin or intermediates of biotin synthesis such as 7-keto-8-amino-pelargonic acid (KAPA) or 7,8-diamino-pelargonic acid (DAPA) in the medium (21). Only the last three steps of the biotin biosynthesis pathway (BIO3, BIO4, and BIO2) have been found in S288C, and steps before KAPA synthesis are absent or have not been elucidated ( Fig.…”

Section: Biotin (Vitamin H) Is An Essential Nutrient For All Organismsmentioning

confidence: 99%

“…It is a cofactor of many enzymes involved in carboxylation reactions, such as gluconeogenesis, amino acid metabolism, fatty acid biosynthesis, and energy metabolism (21). Biotin is synthesized in many organisms from lower prokaryotes to higher plants including Escherichia coli and Arabidopsis thaliana, and its biosynthesis pathways have been well studied (2,5,7,9,11).…”

Section: Biotin (Vitamin H) Is An Essential Nutrient For All Organismsmentioning

confidence: 99%

Identification and Characterization of a Novel Biotin Biosynthesis Gene inSaccharomyces cerevisiae

Itō

Shimoi

2005

Appl Environ Microbiol

View full text Add to dashboard Cite

Yeast Saccharomyces cerevisiae cells generally cannot synthesize biotin, a vitamin required for many carboxylation reactions. Although sake yeasts, which are used for Japanese sake brewing, are classified as S. cerevisiae, they do not require biotin for their growth. In this study, we identified a novel open reading frame (ORF) in the genome of one strain of sake yeast that we speculated to be involved in biotin synthesis. Homologs of this gene are widely distributed in the genomes of sake yeasts. However, they are not found in many laboratory strains and strains used for wine making and beer brewing. This ORF was named BIO6 because it has 52% identity with BIO3, a biotin biosynthesis gene of a laboratory strain. Further research showed that yeasts without the BIO6 gene are auxotrophic for biotin, whereas yeasts holding the BIO6 gene are prototrophic for biotin. The BIO6 gene was disrupted in strain A364A, which is a laboratory strain with one copy of the BIO6 gene. Although strain A364A is prototrophic for biotin, a BIO6 disrupted mutant was found to be auxotrophic for biotin. The BIO6 disruptant was able to grow in biotin-deficient medium supplemented with 7-keto-8-amino-pelargonic acid (KAPA), while the bio3 disruptant was not able to grow in this medium. These results suggest that Bio6p acts in an unknown step of biotin synthesis before KAPA synthesis. Furthermore, we demonstrated that expression of the BIO6 gene, like that of other biotin synthesis genes, was upregulated by depletion of biotin. We conclude that the BIO6 gene is a novel biotin biosynthesis gene of S. cerevisiae.

show abstract

“…Biotin (vitamin H) is a cofactor of many enzymes related to carboxylation reactions. Typical S. cerevisiae strains cannot synthesize biotin and must incorporate it from an extracellular medium (17). Thus, elevated expression of biotin biosynthetic and transport genes has been observed during the cell growth phase of wine (22) (no.…”

mentioning

confidence: 99%

Global Gene Expression Analysis of Yeast Cells during Sake Brewing

Zheng

Araki

et al. 2006

Appl Environ Microbiol

View full text Add to dashboard Cite

During the brewing of Japanese sake, Saccharomyces cerevisiae cells produce a high concentration of ethanol compared with other ethanol fermentation methods. We analyzed the gene expression profiles of yeast cells during sake brewing using DNA microarray analysis. This analysis revealed some characteristics of yeast gene expression during sake brewing and provided a scaffold for a molecular level understanding of the sake brewing process.

show abstract

Microbiological activity of biotin-vitamers.

Cited by 21 publications

References 26 publications

Vitamin requirements and biosynthesis in Saccharomyces cerevisiae

Vitamin requirements and biosynthesis in Saccharomyces cerevisiae

Identification and Characterization of a Novel Biotin Biosynthesis Gene inSaccharomyces cerevisiae

Global Gene Expression Analysis of Yeast Cells during Sake Brewing

Contact Info

Product

Resources

About