Microbiological and biochemical characteristics of inflammatory tissues in the periodontium

Abstract

Objective. To investigate bacterial populations in subgingival and supragingival plaque samples of patients with inflammatory periodontal diseases and activities of the lysosomal enzymes – lysozyme, alkaline phosphatase, and b-glucuronidase – in peripheral venous blood, in gingival crevicular fluid, and mixed nonstimulated saliva. Methods and materials. The study included 60 patients with inflammatory periodontal diseases without any internal pathology and 24 periodontally healthy subjects. Molecular genetic assay (Micro-IDent plus, Germany) for complex identification of additional six periodontopathic bacteria was applied. The activity of lysozyme was determined turbidimetrically, the activity of alkaline phosphatase – spectrophotometrically with a “Monarch” biochemical analyzer, the activity b-glucuronidase – according to the method described by Mead et al. and modified by Strachunskii. Results. A statistically significant association between clinical and bacteriological data was found in the following cases: gingival bleeding in the presence of Eubacterium nodatum, Eikenella corrodens, Capnocytophaga spp. (P<0.01); pathological periodontal pockets in the presence of Peptostreptococcus micros (α≤0.05 and β≤0.2), Fusobacterium nucleatum (α≤0.05 and β≤0.2), Campylobacter rectus (α≤0.05 and β≤0.2), and Capnocytophaga spp. (P<0.05); and satisfactory oral hygiene in the presence of all microorganisms investigated (P<0.05). The activity of lysozyme in gingival crevicular fluid and mixed nonstimulated saliva indicates the severity of periodontal inflammation. Based on clinical data, in assessing the amount of lysozyme in mixed nonstimulated saliva, sensitivity and specificity of 100% was found. Increased activities of lysozyme, alkaline phosphatase, and b-glucuronidase were found in peripheral venous blood of patients with inflammatory periodontal disease as compared to control group. Conclusions. The main principles of the treatment of periodontal inflammatory diseases should be based on microorganism elimination, creation of individual treatment means affecting microflora in the mouth and immune system of macroorganisms.

Фітопрепарат «Ресверазин» У Комплексному Лікуванні Генералізованого Пародонтиту

“…Відомо, що ротова порожнина є збалансова-ною біологічною системою, порушення рівно-ваги в якій між бактеріальним симбіозом та резистентністю тканин ротової порожнини сприяє розвитку захворювань пародонта [2,7,10]. Мікроорганізми ротової порожнини -ана-ероби та грамнегативні бактерії, проникаючи вглиб тканин, виділяють ендотоксини, фер-менти та продукти життєдіяльності, виявля-ють агресивну дію в ділянці бактеріальної ін-вазії.…”

Activity of Neutrophil β-Glucuronidase in Diabetic and Nondiabetic Patients With Chronic Generalized Periodontitis and Healthy Subjects

“…A number of lysosomal enzymes of neutrophilic leukocytes can be found not only inside the cell but also can be secreted extracellularly, killing microorganisms that are not destroyed during phagocytosis due to their size or for other reasons (9). Neutrophilic leukocytes can be stimulated by bacterial lipopolysaccharides and other toxins, cytokines, and the 3 fraction of activated complement (C3).…”