Microbiological and Immunological Effects of Monomer Free Thermosens and Nano Zirconia Oxide Reinforced Denture Base Resins on Controlled Diabetic Denture Wearers

Abstract

Background:The overall success of denture base resins depends on both mechanical behaviour and biocompatibility, especially in the rehabilitation of diabetic patients. The growth and nourishment of microorganisms is enhanced in diabetes mellitus specially in presence of dentures resulting in denture stomatitis. This study was prompted to assess the microbiological growth and the development of salivary immunoglobulin A (s-IgA) in relation to newly introduced denture base materials used in the rehabilitation of diabetic patients.Materials and Methods: Forty eight completely edentulous patients were selected and divided into two groups: group (A), which included controlled diabetic patients and group (B) included nondiabetic patients. Each group was further divided into four sub groups according to the denture base material. Sub group I, patients were received thermoplastic Vertex TM ThermoSens denture bases; sub group II, patients were received heat cure acrylic resin dentures reinforced with nano zirconia oxide particles (nano-ZrO2); sub group III, patients were received conventional heat cure acrylic resin dentures; where sub group VI, patients were received conventional heat cure acrylic resin dentures relined with self-cure acrylic resin. For each participant, non-stimulated saliva samples were collected before denture insertion, and one, two and three months after wearing dentures. Samples were inoculated and incubated both aerobically and anaerobically on selective media for Streptococcus mutans, Lactobacilli and Candida. Then, isolated microorganisms were identified, the characteristic colony forming units per millilitre (CFUs/ml), were calculated and the salivary immunoglobulin A level was measured by Enzyme-linked immunosorbent assay (ELISA). Results:The results revealed increase in the CFU values of Candida, Streptococcus mutans, Lactobacilli and the level of salivary immunoglobulin A in all the assessed patients following denture insertion. However, significant lower values were evident in patients rehabilitated with ThermoSens and nano-ZrO2 reinforced bases compared to heat cure acrylic and self-cure relined bases. Significant increase in CFU values and level of s-IgA were found in diabetic compared to non-diabetic patients after insertion of all denture bases.

Patient satisfaction and oral health‐related quality of life for four implant‐assisted mandibular overdentures fabricated with CAD/CAM milled poly methyl methacrylate, CAD/CAM‐milled poly ether ether ketone, or conventional poly methyl methacrylate: A crossover clinical trial

“…To overcome these shortages and enhance the quality of the final prostheses, many processing techniques and materials have been emerged and improved over time. [17][18][19] In 1994, the first CAD/ CAM complete denture construction was reported. 20 Since then, numerous commercial systems for designing and fabricating CAD/ CAM complete dentures have been emerged using either rapid prototyping or milling technology.…”

“…To overcome these shortages and enhance the quality of the final prostheses, many processing techniques and materials have been emerged and improved over time 17–19 . In 1994, the first CAD/CAM complete denture construction was reported 20 .…”