Microbiological and Sybr® Green real-time PCR detection of major Fusarium head blight pathogens on wheat ears

Moradi, Mohammad; Oerke, E. C.; Steiner, Ulrike; Tesfaye, Dawit; Schellander, K.; Dehne, H. W.

doi:10.1134/s0026261710050097

Cited by 20 publications

(8 citation statements)

References 26 publications

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“…The standard curve constructed using dilution series of pure F. culmorum DNA showed very high correlation between Ct values and the logarithm of the amount of input DNA (r 2 = 0.998) comparable with the results of Leišová et al (2006) and Moradi et al (2010). The contents of F. culmorum DNA in analysed samples expressed in nanograms of pathogen DNA per 1 g of wheat seeds and the infection per cent calculated as (Fusarium DNA/ total DNA) × 100 as given in Table 1.…”

Section: Resultssupporting

confidence: 56%

“…SYBR-Green real-time PCR was successfully used for the specific detection of trichothedene-producing Fusarium spp. (Schnerr et al 2001;Moradi et al 2010) and also more specific Taq-Man real-time PCR was used to quantify main Fusarium species (Reischer et al 2004;Waalwijk et al 2004;Leišová et al 2006;Yli-Mattila et al 2008). Leišová et al (2006) found a significant correlation between the amounts of F. culmorum DNA (expressed by transformed threshold cycle (Ct) values) detected by Taq-Man real-time PCR and the content of DON detected by ELISA in barley, while in wheat the relationship was rather complicated and influenced by environmental conditions.…”

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confidence: 99%

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Evaluation of Slovak Wheat Cultivars for Fusarium Culmorum Infection by Real-Time PCR and By Conventional Assays

Hudcovicová¹,

Šliková²,

Šudyová³

et al. 2012

Agriculture (Pol'nohospodárstvo)

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-ŠLIKOVÁ, S. -ŠUDYOVÁ, V. -HAUPTVOGEL, P.: Evaluation of Slovak wheat cultivars for Fusarium culmorum infection by real-time PCR and by conventional assays. Agriculture (Poľnohospodárstvo), vol. 58, 2012, no. 1, pp. 18-24. Ing. Martina Hudcovicová, PhD., Ing. Svetlana Šliková, PhD., Ing. Pavol Hauptvogel, PhD., Plant Production Research Center, 921 68 Piešťany, Bratislavská cesta 122, Slovak Republic. E-mail: hudcovicova@vurv.sk vars. An average kernel contamination with DON in the tested cultivars was 24.9 mg/kg and an average amount of F. culmorum DNA was 10,528 ng/g. The old cultivars accumulated 35.9% less DON and 51% less F. culmorum DNA than modern cultivars. The positive correlation coefficients were significant with AUDPC and DON content, and with the amount of F. culmorum DNA and DON content and AUDPC (P < 0.01). Correlation coefficients were higher when we used quantification of F. culmorum DNA expressed in infection per cent. Our study has confirmed that real-time polymerase chain reaction is very suitable method for evaluation of wheat cultivars for F. culmorum infection and presents less time-consuming, more sensitive and more specific assay than conventional assays.

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Section: Resultssupporting

confidence: 56%

mentioning

confidence: 99%

Evaluation of Slovak Wheat Cultivars for Fusarium Culmorum Infection by Real-Time PCR and By Conventional Assays

Hudcovicová¹,

Šliková²,

Šudyová³

et al. 2012

Agriculture (Pol'nohospodárstvo)

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“…TUB, UBC, GzPRS16 were used as candidate reference genes for qPCR studies in F. graminearum under different culture conditions (Kim, 2011). Gao has been used as one of the candidate genes for PCR based detection of F. graminearum (Niessen and Vogel, 2010) whereas, MGBGRA has been used as a Taqman probe to differentiate four different species of fusarium using qPCR analysis (Moradi et al, 2010). Tri5 is responsible for catalyzing the first step in the biosynthetic pathway of trichothecenes, which acts as a pathogenicity factor, in disease development (Bai and Shaner, 2004).…”

Section: Tri6_10 Primer Discriminated the Control (Uninfected) From Tmentioning

confidence: 99%

“…Based on the existing literature (Biazio et al, 2008;Horevaj et al, 2011;Kim, 2011;Moradi et al, 2010), six different F. graminearum specific genes namely Gao, galactose oxidase; UBC, ubiquitin conjugating enzyme; TUB, b-tubulin; MGBGRA, minor groove binder specific to F. graminearum; GzRPS16, Gibberella zea mitochondrial ribosomal protein S16 and Tri6, transcription factor regulating trichothecene biosynthesis were selected to discriminate the genotypes infected with F. graminearum and mock samples. Additionally, one more in-house gene Tri5 (trichodiene synthase) (GenBank accession number JF966257.1) was tested for the same purpose.…”

Section: Selection Of Fusarium Specific Gene For Biomass Quantificationmentioning

confidence: 99%

Real-time quantitative PCR based method for the quantification of fungal biomass to discriminate quantitative resistance in barley and wheat genotypes to fusarium head blight

Kumar

Karre

Dhokane

et al. 2015

Journal of Cereal Science

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“…It can also be used to monitor fungal growth before (external) symptoms are present and to study the threshold levels for disease development (Demontis et al 2008;Malvick and Impullitti 2007;Moradi et al 2010;Rossi et al 2007). The ability to detect very small amounts of a pathogen can also be useful when evaluating the resistance of cultivars against certain pathotypes (Markakis et al 2009) or determining whether the pathogen can survive or even accumulate in non-symptomatic plants or non-hosts (Zellerhoff et al 2006).…”

mentioning

confidence: 99%

Real-time PCR mediated monitoring of Fusarium foetens in symptomatic and non-symptomatic hosts

Huvenne¹,

Debode²,

Maes³

et al. 2011

Eur J Plant Pathol

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Fusarium foetens is a recently described aggressive vascular pathogen of Begonia x hiemalis. Since 2004, it has caused severe losses for Begonia growers in Northern Europe and North America. F. foetens is likely to be of exotic origin. Little is known about the accumulation of the fungus in Begonia plants before and during symptom expression and about its host range. We have optimised a molecular detection method for F. foetens by only using the plant part containing the largest amount of the pathogen and by optimising the tissue maceration and DNA extraction techniques. This allowed a reliable detection limit of 2310 spore equivalents per plant and a theoretical detection limit of as low as 84 to 167 spore equivalents per plant. Using this method, we demonstrated exponential accumulation of F. foetens DNA in Begonia roots, resulting in symptoms at a threshold of approximately 10 7 spore equivalents and levelling off at 10 9 spore equivalents per plant. The observed rate of accumulation and the amount of pathogen DNA in non-symptomatic plants can be combined to determine whether the cuttings were infected after delivery at the Begonia nursery and to calculate the estimated timing of symptom development. To test the host range, we applied the optimised molecular detection technique. During these tests, only Begonia x hiemalis plants became symptomatic, but many other plant species supported growth of the pathogen. This information can be used to aid pathogen control and has implications for pest risk assessment.

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Microbiological and Sybr® Green real-time PCR detection of major Fusarium head blight pathogens on wheat ears

Cited by 20 publications

References 26 publications

Evaluation of Slovak Wheat Cultivars for Fusarium Culmorum Infection by Real-Time PCR and By Conventional Assays

Evaluation of Slovak Wheat Cultivars for Fusarium Culmorum Infection by Real-Time PCR and By Conventional Assays

Real-time quantitative PCR based method for the quantification of fungal biomass to discriminate quantitative resistance in barley and wheat genotypes to fusarium head blight

Real-time PCR mediated monitoring of Fusarium foetens in symptomatic and non-symptomatic hosts

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