Microbiological assay with Bacillus licheniformis for the easy detection of quinolones in milk

Tumini, Melisa; Nagel, Orlando G.; Molina, M.P.; Althaus, R. L.

doi:10.1016/j.idairyj.2016.08.008

Cited by 36 publications

(18 citation statements)

References 22 publications

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“…In addition, camel milk-based creams decrease inflammation of dermatological autoimmune diseases [ 3 - 6 ]. Recently, cases of mastitis refractory to any type of treatment have been reported frequently [ 9 - 11 ]. A wide range of Gram-positive spore-forming bacilli proved to be part of the teat normal microflora [ 12 - 15 ], commonly isolated from both raw and pasteurized milk as environmental commensals [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…A wide range of Gram-positive spore-forming bacilli proved to be part of the teat normal microflora [ 12 - 15 ], commonly isolated from both raw and pasteurized milk as environmental commensals [ 14 ]. On the other hand, probiotic Bacillus species including B. subtilis , B. cereus , B. alvei , B. megaterium, B. Clausii , and B. pumilus are already marketed as gastrointestinal enhancers for both humans and animals and release antibacterial lipopeptides [ 11 ]. In addition, their bacteriocins are utilized as biocontrol agents of some infections as well as insect vectors [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Nonetheless, B. cereus was held responsible for hemorrhagic disease, when it was isolated from a 9-year-old she-camel that aborted; from the placenta and organs of the fetus (spleen, liver, and intestines) [ 15 , 16 ]. Since 1970s, Bacillus licheniformis proved to be involved in suppurative inflammation leading to abortion and/or mastitis, from mild to gangrenous, with frequent rapid fatalities [ 11 ]. Regardless that details of pathogenesis are yet unknown, previous outcomes were recorded in animal fed on poor quality silage and/or contaminated intramammary injections [ 15 ], where their pathogenicity is hooked on the discrepancy in host immunity [ 4 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Multifactorial mastitis has serious implications on public health [ 13 ], hence, serving as a media for transmission of various zoonotic diseases [ 13 , 14 ]. During infection, milk quality varies from normal to pink-tinged with blood in mild or early cases; later on, it became rusty-brown-colored discharge indicating the establishment of recurrent mastitis which is the typical prognosis of B. licheniformis infection [ 11 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, abuse use of antibiotics will enhance the development of drug resistance and failure to treat; at that point, the lactating animal will be culled from herd [ 7 , 15 ]. Public health and food safety associated with dairy products are reliant on milk quality (residuals and bacterial contents) and animal welfare, which necessities rapid and accurate identification of bacterial species [ 11 ]. Alternative analysis of species-specific stretches of the genomes, which are recommended for identification of atypical bacterial variants in phenotype, was developed [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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The clinical impact of antimicrobial resistance genomics in competition with she-camels recurrent mastitis metabolomics due to heterogeneous Bacillus licheniformis field isolates

2017

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Background and Aim::Recently, cases of mastitis refractory to treatment have been reported frequently. There are limited routine laboratory investigations on Camelidae infections. Mastitis has been estimated to affect more than 25% of lactating she-camel with up to 70% milk loss. The details of Bacillus spp. pathogenesis in mastitis are not yet fully described. The present study is the first detailed phenotypic and genotypic characterization of Bacillus licheniformis isolates from recurrent mastitic she-camels with sepsis in Egypt.Materials and Methods:The udders of 100 she-camels were investigated, samples collected from smallholders’ farmers in 10 localities within three governorates in Egypt: Marsa Matrouh, Giza, and Sharkia governorates. The pathogens ascend from udder inducing abortion at different trimesters of pregnancy. Polymerase chain reactions-mediated proofs of identity were applied for diagnostic and taxonomic purposes, where the 16S rRNA gene sequence and the β subunit of RNA polymerase encoding gene rpoB are the molecular targets.Results::The genetic elements classified the subspecies to B. licheniformis 61.4%, in addition to, Corynebacterium bovis 29.8%. The somatic cell count (≤1×107 cells/ml) and California mastitis test reactivity (+3 or +4) of milk clinically classified the she-camels population (n=100) under investigation into 50, 20, and 30 as healthy, subclinical, and clinical mastitic she-camels, respectively. During bacterial isolation, 80 species were noticed, of which 71.25% (57/80) and 28.75% (23/80) were Gram-positive and negative, respectively, in two clinical forms: Single (40%, n=16/40) and mixed (60%, n=34/40) bacterial infections. In vitro, 100% sensitivity for gentamycin (10 µg) and ofloxacin (5 µg) was noted; however, it was reduced to 50%. Moreover, during in vivo treatments, cloxacillin (5 µg) upraised as the most effective alternative with 90% sensitivity.Conclusion:Neither recurrent mastitis nor Bacillus species are thoroughly investigated with regard to reproduction performance in Egypt and the usefulness of these strains as antimastitis probiotics. Both persistent bacteremia and dormant endospores were formed but unaffected by standard schemes of antimicrobials injections which proposed the risk of pathogenic bacilli contaminating row milk from apparently healthy she-camel. The discrepancies between treatment results were induced by the resistance that started to develop by the organisms due to frequent and/or faulty use of applied antibiotics.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The clinical impact of antimicrobial resistance genomics in competition with she-camels recurrent mastitis metabolomics due to heterogeneous Bacillus licheniformis field isolates

2017

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Aptamer‐Based Antibiotic Electrochemical Detection Platform Using Portable Plastic Gold Electrode and Multichannel Chip

Bao,

Cai,

Liu

et al. 2023

Chin. J. Chem.

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The development of accurate, rapid and practical electrochemical sensing technology for antibiotic detection has an important application prospect in many fields such as food safety, environmental monitoring, medical and health care. In this research, kanamycin specific nucleic acid aptamer and its partially complementary short chain were self‐assembled onto the surface of an integrated portable plastic gold electrode (PGE) by Au‐S bond, and a simple kanamycin electrochemical biosensor interface was constructed. At the same time, the epoxy resin channel layer was designed, and the auxiliary instrument was set up independently in the laboratory, which could help to measure and analyze eight groups of samples automatically in turn. The quantitative analysis results showed that in 20 minutes, the sensor had a good linear relationship between the peak current change of square wave voltammetry and the negative logarithm of kanamycin concentration when kanamycin concentration was in the range of 1‐1000 μM, and the detection limit could reach 0.40 μM. In addition, the discrimination could be achieved within 5 minutes in the real‐time analysis mode. The sensor had a simple construction method, good selectivity and stability, and could be used for rapid or real‐time detection of kanamycin residues in actual water samples, which provided a new direction for the practical detection of kanamycin in environmental water samples.This article is protected by copyright. All rights reserved.

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A microbial inhibition assay in microplates using Bacillus licheniformis for detection of enrofloxacin and sulfamethazine in chicken spiked kidney, liver and muscle tissues

Mirzaie,

Jamiri,

Javanmard Dakheli

et al. 2023

Veterinary Medicine & Sci

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BackgroundThe presence of antimicrobial drugs residues in animal products at levels higher than the maximum residue level (MRL) may have adverse effects on consumer health such as allergic reactions and resistance development. Therefore, it is necessary to monitor animal products for the presence of antimicrobial residues.ObjectivesThe aim of this study was to evaluate the detection limit of microbial inhibition assay (MIA) in microplate by using of Bacillus licheniformis as indicator microorganism for two antibiotics, enrofloxacin (ENR) and sulfamethazine (SMT), in broiler chicken's kidney, liver and muscle tissue samples.MethodsSpiked tissues samples for the two antibiotics were analysed separately by this method. The results of the assay were evaluated by the determination of the absorbance after mean 3.47 h of incubation at 45°C.ResultsResults showed that the detection limits of MIA for ENR and SMT in kidney (124.03 and 23.21 μg/kg, respectively) and liver (90.02 and 62.03 μg/kg, respectively) as well as SMT in muscle (46.95 μg/kg) were lower than EU (European Union) – MRL, whereas the detection limit for ENR in muscle was slightly higher than MRL (136.3 μg/kg compared to 100 μg/kg MRL). Furthermore, the MIA in the current study was found to be more sensitive to SMT than ENR (92% and 88% sensitivity rate, respectively). No false‐positive was observed in the assay.ConclusionsBased on the results, the MIA investigated in this study had the potential to detect ENR and SMT residues in broiler chicken kidney, liver and muscle tissues at levels below or close to EU – MRL but offered lower capability for the detection of ENR compared with SMT in kidney and muscle tissue samples.

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Microbiological assay with Bacillus licheniformis for the easy detection of quinolones in milk

Cited by 36 publications

References 22 publications

The clinical impact of antimicrobial resistance genomics in competition with she-camels recurrent mastitis metabolomics due to heterogeneous Bacillus licheniformis field isolates

The clinical impact of antimicrobial resistance genomics in competition with she-camels recurrent mastitis metabolomics due to heterogeneous Bacillus licheniformis field isolates

Aptamer‐Based Antibiotic Electrochemical Detection Platform Using Portable Plastic Gold Electrode and Multichannel Chip

A microbial inhibition assay in microplates using Bacillus licheniformis for detection of enrofloxacin and sulfamethazine in chicken spiked kidney, liver and muscle tissues

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