Microbiological Assays and Bioautography of Maytansine and Its Homologues

Hanka, L. J.; Barnett, Michael

doi:10.1128/aac.6.5.651

Cited by 20 publications

(7 citation statements)

References 3 publications

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“…79) While these test systems make use of the antibiotic activity of maytansinoids, protozoa can be used to test for their antitubulinic activity. [80][81][82] Protozoa may be equipped with axopods (cilia) containing bundles of microtubules.…”

Section: )mentioning

confidence: 99%

Recent Developments in the Maytansinoid Antitumor Agents

et al. 2004

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Section: )mentioning

confidence: 99%

Recent Developments in the Maytansinoid Antitumor Agents

et al. 2004

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“…The strains used were Staphylococcus aureus (CBAM-026), Bacillus cereus (CBAM-289), Enterococcus faecalis (CBAM-309) Pseudomonas aeuriginosa (CBAM-024) and the fungal strains of Candida albicans (CFAM-1132) and Penicillium avellaneum. The fungus P. avellaneum is an species which has been used by many researchers as a target of anticancer and antifungal compounds ( Floss et al , 2004 ; Hanka and Barnett 1974 ; Kittakoop et al , 2009 ).…”

Section: Methodsmentioning

confidence: 99%

Endophytic fungi from Myrcia guianensis at the Brazilian Amazon: distribution and bioactivity

Banhos

Souza

Andrade

et al. 2014

Braz. J. Microbiol.

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Beneficial interactions between plants and microorganisms have been investigated under different ecological, physiological, biochemical, and genetic aspects. However, the systematic exploration of biomolecules with potential for biotechnological products from this interaction still is relatively scarce. Therefore, this study aimed the evaluation of the diversity and antimicrobial activity of the endophytic fungi obtained from roots, stems and leafs of Myrcia guianensis (Myrtaceae) from the Brazilian Amazon. 156 endophytic fungi were isolated and above 80% were identified by morphological examination as belonging to the genera Pestalotiopsis, Phomopsis, Aspergillus, Xylaria, Nectria, Penicillium and Fusarium. Fermented broth of those fungi were assayed for antimicrobial activity and four inhibited the growth of Staphylococcus aureus, Enterococcus faecalis, Candida albicans and Penicillium avellaneum. As the strain named MgRe2.2.3B (Nectria haematococca) had shown the most promising results against those pathogenic strains, its fermented broth was fractioned and only its two low polar fractions demonstrated to be active. Both fractions exhibited a minimum bactericidal concentration of 50 μg.mL−1 against S. aureus and a minimum fungicidal concentration of 100 μg.mL−1 against P. avellaneum. These results demonstrate the diversity of fungal genera in M. guianensis and the potential of these endophytic fungi for the production of new antibiotics.

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“…Meanwhile, Yao Xinsheng also selected some antitumor ingredients from a large number of herbal medicines with this model [ 66 ]. LJ Hanka et al developed a quantitative microbiological assay and a bioautography system with Penicillium avellaneum UC-4376 for the antitumor drug maytansine and its homologues maytanprine and maytanbutine [ 67 ]. However, this method uses micro-dilution over separation, tracking and selecting; therefore, investigators cannot determine whether there are active antitumor components in crude extract samples in one step [ 68 ].…”

Section: Biological Applicationsmentioning

confidence: 99%

An Evolving Technology That Integrates Classical Methods with Continuous Technological Developments: Thin-Layer Chromatography Bioautography

et al. 2021

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Thin-layer chromatography (TLC) bioautography is an evolving technology that integrates the separation and analysis technology of TLC with biological activity detection technology, which has shown a steep rise in popularity over the past few decades. It connects TLC with convenient, economic and intuitive features and bioautography with high levels of sensitivity and specificity. In this study, we discuss the research progress of TLC bioautography and then establish a definite timeline to introduce it. This review summarizes known TLC bioautography types and practical applications for determining antibacterial, antifungal, antitumor and antioxidant compounds and for inhibiting glucosidase, pancreatic lipase, tyrosinase and cholinesterase activity constitutes. Nowadays, especially during the COVID-19 pandemic, it is important to identify original, natural products with anti-COVID potential compounds from Chinese traditional medicine and natural medicinal plants. We also give an account of detection techniques, including in situ and ex situ techniques; even in situ ion sources represent a major reform. Considering the current technical innovations, we propose that the technology will make more progress in TLC plates with higher separation and detection technology with a more portable and extensive scope of application. We believe this technology will be diffusely applied in medicine, biology, agriculture, animal husbandry, garden forestry, environmental management and other fields in the future.

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Microbiological Assays and Bioautography of Maytansine and Its Homologues

Abstract: A quantitative microbiological assay and a bioautography system with Penicillium avellaneum UC-4376 was developed for the antitumor drugs maytansine, and its homologues, maytanprine and maytanbutine. The susceptibility of the assay is 1.5 μg/ml.

Cited by 20 publications

References 3 publications

Recent Developments in the Maytansinoid Antitumor Agents

Recent Developments in the Maytansinoid Antitumor Agents

Endophytic fungi from Myrcia guianensis at the Brazilian Amazon: distribution and bioactivity

An Evolving Technology That Integrates Classical Methods with Continuous Technological Developments: Thin-Layer Chromatography Bioautography

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