Microbiological Characteristics of Dungeness Crab (Cancer magister)1

Lee, J. S.; Pfeifer, Doreen

doi:10.1128/aem.30.1.72-78.1975

Cited by 22 publications

(9 citation statements)

References 7 publications

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“…Incubation at 20 to 25°C in the air has been used by most previous investigators to isolate and identify microorganisms from seafood (1, 2, 14). The relative numbers of the different genera found on our plates incubated under these conditions are in general agreement with previous studies (12,14). Strictly respiratory bacteria, especially Pseudomonas spp., Acinetobacter spp., and Moraxella spp., were found to predominate.…”

Section: Resultssupporting

confidence: 93%

“…not complete since the microbial counts increased during MA storage, as has been reported by other investigators (1, 2, 10). The day 0 microbial flora was similar to that normally isolated from fresh marine animals (12,14,15,22).…”

Section: Discussionsupporting

confidence: 57%

“…Media. The media used were similar to those used by Lee and Pfeifer (14), except for the bromthymol blue-teepol agar, mannitol salt agar (Difco Laboratories, Detroit, Mich.) and MacConkey agar (Difco), which were included for the selective isolation and differentiation of Vibrio spp., Staphylococcus spp., and Enterobacteriaceae, respectively. The composition of the bromthymol blue-teepol agar is as follows (grams per liter): yeast extract, 3.0; peptone, 10.0; sodium chloride, 40.0; sucrose, 20.0; bromthymol blue, 0.04; thymol blue, 0.04; teepol (Particle Data Laboratories, Ltd., Elmhurst, Ill.), 2.0 ml; agar, 15.0.…”

Section: Downloaded Frommentioning

confidence: 99%

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Microbiological Analysis of Rock Cod ( Sebastes spp.) Stored Under Elevated Carbon Dioxide Atmospheres

Mokhele

Johnson

Barrett

et al. 1983

Appl Environ Microbiol

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The numbers and types of microorganisms on fresh rock cod fillets and fillets stored in air or in a modified atmosphere (MA; 80% CO2, 20% air) at 4°C were compared. Samples were analyzed after 0, 7, 14, and 21 days of storage. The isolation plates were incubated aerobically, anaerobically, or under MA at 4, 20, or 35°C. After 7 days of storage in air, the fillets were obviously spoiled and had a 3to 4-log cycle increase in microbial counts. Plate counts increased more slowly on MA-stored fillets. After 21 days, the counts on the latter had increased only 2 log cycles, and the fillets did not seem spoiled. The microbial flora changed greatly during MA storage. Only Lactobacillus spp. (70%) and an Aeromonas sp.like isolate (30%) were found on plates incubated aerobically at 4 and 20°C, and only Lactobacillus spp. was found on plates incubated aerobically and anaerobically at 35 and at 20°C under MA. Isolation plates incubated at 20°C in air gave the highest counts in the shortest incubation time and the greatest diversity of bacterial types recovered. No Vibrio parahaemolyticus, Staphylococcus aureus, or Clostridium botulinum type E were isolated from the fresh or MA-stored fillets.

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Section: Resultssupporting

confidence: 93%

Section: Discussionsupporting

confidence: 57%

Section: Downloaded Frommentioning

confidence: 99%

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Microbiological Analysis of Rock Cod ( Sebastes spp.) Stored Under Elevated Carbon Dioxide Atmospheres

Mokhele

Johnson

Barrett

et al. 1983

Appl Environ Microbiol

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“…We have previously noted the selective elimination of Moraxella spp. from smoked salmon (7), but the opposite was observed with the cooked Dungeness crab (8).…”

Section: Resultsmentioning

confidence: 99%

Microbiological characteristics of Pacific shrimp (Pandalus jordani)

Lee¹,

Pfeifer²

1977

Appl Environ Microbiol

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Microorganisms associated with Pacific shrimp (Pandalus jordani) were isolated and identified. Those on the iced raw shrimp, which yielded an average count of 1.6 x 106, were predominantly Moraxella, Pseudomonas, Acinetobacter, Arthrobacter, and Flavobacterium-Cytophaga spp. The blanching and peeling reduced the microbial level to 3.3 x 104 and also selectively eliminated Moraxella spp. The microbial flora changed after each processing sequence, and the heat sensitivity and growth characteristics of the representative microbial groups suggested that the presence of Arthrobacter and Acinetobacter spp. in peeled shrimp may indicate inadequate cleaning of raw shrimp or a shorter blanching time. The presence of Moraxella and Flavobacterium-Cytophaga spp. would indicate the degree of secondary contamination, and the presence of Pseudomonas spp. would indicate the shelf-age of the processed shrimp.

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“…Similar observations were made by Collwell, Wicks & Tubiash (1975) and Lightner (1977) indicating that the haemolymph is not sterile and the host immune system controls low‐level bacterial infection (Brock & Lightner 1990). In contrast, others (Bang 1970; Lee & Pfeiffer 1975; Johnson 1976) have reported that the haemolymph is sterile. Previous studies with freshly caught N. norvegicus have reported the presence of Achromobacter, Acinetobacter, Cytophaga, Micrococcus, Pseudomonas, Alteromonas and Vibrio species (Cann 1970; Hobbs 1971; Montero 1998).…”

Section: Discussionmentioning

confidence: 87%

Idiopathic muscle necrosis in the Norway lobster, Nephrops norvegicus (L.): aetiology, pathology and progression to bacteraemia

Ridgway

Stentiford

Taylor

et al. 2007

Journal of Fish Diseases

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The pathology and progression of idiopathic muscle necrosis (IMN) in Nephrops norvegicus and possible aetiologies have been investigated. Trawl capture, aerial exposure and handling initiate IMN, and the condition can be induced through periods of aerial exposure alone, in the absence of trawling. Within 24-48 h after trawl capture IMN progresses to a multi-species bacterial septicaemia, with moribund animals exhibiting clinical signs. The aetiology of this condition has been examined using molecular (16S rRNA gene sequencing) and biochemical (standard taxonomic assays, Biolog) criteria to characterize bacterial isolates from moribund and healthy animals. Histopathology of the IMN phase reveals a loss of sarcomeric structure with necrotic lesions containing pyknotic nuclei, fragments of myofibrils and connective tissue elements. In the bacterial phase there is extensive loss of abdominal muscle structure, and the presence of rod-shaped Gram-negative bacteria in the degrading tissues. The results demonstrate that the IMN condition is connected to stressful conditions imposed on N. norvegicus, but involves no pathogenic agents. This is followed by an opportunistic bacterial infection that causes further tissue spoilage. It is believed that the primary cause of both IMN and bacteraemia is imposed stress, but they are expressed in different time courses.

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Microbiological Characteristics of Dungeness Crab (Cancer magister)1

Cited by 22 publications

References 7 publications

Microbiological Analysis of Rock Cod ( Sebastes spp.) Stored Under Elevated Carbon Dioxide Atmospheres

Microbiological Analysis of Rock Cod ( Sebastes spp.) Stored Under Elevated Carbon Dioxide Atmospheres

Microbiological characteristics of Pacific shrimp (Pandalus jordani)

Idiopathic muscle necrosis in the Norway lobster, Nephrops norvegicus (L.): aetiology, pathology and progression to bacteraemia

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