Microbiological contamination and resistance genes in biofilms occurring during the drinking water treatment process

Farkas, Anca; Butiuc-Keul, Anca; Ciatarâş, Dorin; Neamțu, Călin; Crăciunaş, C.; Podar, Dorina; Drăgan-Bularda, M.

doi:10.1016/j.scitotenv.2012.11.068

Cited by 45 publications

(18 citation statements)

References 47 publications

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“…In contrast to qacEΔ 1 , the gene qacE was only detected by PCR-Southern blot hybridization in the samples from a biofilter used to degrade pesticides as well as in one replicate of potato rhizosphere of LL soil and in lettuce rhizosphere of DS soil, indicating a lower distribution of this gene variant in the environment. A low abundance of qacE was also observed by Chuanchuen et al (2007) who could detect qacEΔ 1 but not qacE genes in Salmonella enterica isolates from poultry and swine as well as by Farkas et al (2013) who could detect qacEΔ 1 but not qacE genes in biofilm samples collected at a drinking water treatment plant facility. Furthermore, in clinical isolates of Gram-negative bacteria qacEΔ 1 was found in 10% of the isolates ( n = 103) while qacE was only found in one P. aeruginosa strain (Kücken et al, 2000).…”

Section: Discussionsupporting

confidence: 56%

Widespread dissemination of class 1 integron components in soils and related ecosystems as revealed by cultivation-independent analysis

et al. 2014

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Class 1 integrons contribute to the emerging problem of antibiotic resistance in human medicine by acquisition, exchange, and expression of resistance genes embedded within gene cassettes. Besides the clinical setting they were recently reported from environmental habitats and often located on plasmids and transposons, facilitating their transfer and spread within bacterial communities. In this study we aimed to provide insights into the occurrence of genes typically associated with the class 1 integrons in previously not studied environments with or without human impact and their association with IncP-1 plasmids. Total community DNA was extracted from manure-treated and untreated soils, lettuce and potato rhizosphere, digestates, and an on-farm biopurification system and screened by PCR with subsequent Southern blot hybridization for the presence of the class 1 integrase gene intI1 as well as qacE and qacEΔ 1 resistance genes. The results revealed a widespread dissemination of class 1 integrons in the environments analyzed, mainly related to the presence of qacEΔ 1 genes. All 28 IncP-1ε plasmids carrying class 1 integrons, which were captured exogenously in a recent study from piggery manure and soils treated with manure, carried qacEΔ 1 genes. Based on the strong hybridization signals in the rhizosphere of lettuce compared to the potato rhizosphere, the abundances of intI1, qacE/qacEΔ 1, and sul1 genes were quantified relative to the 16S rRNA gene abundance by real-time PCR in the rhizosphere of lettuce planted in three different soils and in the corresponding bulk soil. A significant enrichment of intI1 and qacE/qacEΔ 1 genes was confirmed in the rhizosphere of lettuce compared to bulk soil. Additionally, the relative abundance of korB genes specific for IncP-1 plasmids was enriched in the rhizosphere and correlated to the intI1 gene abundance indicating that IncP-1 plasmids might have contributed to the spread of class 1 integrons in the analyzed soils.

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Section: Discussionsupporting

confidence: 56%

Widespread dissemination of class 1 integron components in soils and related ecosystems as revealed by cultivation-independent analysis

et al. 2014

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“…Predominantly, the MGEs investigations are focused on the intI gene encoding the class 1 integronase [11,13].…”

Section: Args Occurring In Arb and Dna Isolated From Tap Watera Reviewmentioning

confidence: 99%

“…Technologies presently applied in water treatment plants do not provide the sufficient ARGs removing levels [11,16,51,57]. The proposed improvements may be the increase in dosages of chlorine and the intensity of UV irradiation or sequential UV/chlorination [9], but these solutions generate high costs.…”

Section: Considered Solutionsmentioning

confidence: 99%

“…This phenomenon is explained, among others, by the association of class 1 integrons to gene cassettes containing antibiotic resistance genes (ARGs). Therefore, investigations of ARGs are frequently supplemented by the intI gene research [11][12][13]. As ARB could cause incurable infections, the constant monitoring of their prevalence should be implemented in all environmental components, including wastewaters, soil, air, and waters [6].…”

Section: Introductionmentioning

confidence: 99%

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The occurrence of antibiotic resistance genes in tap water – a review

Siedlecka

2018

E3S Web Conf.

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Abstract. The study presents a review of the occurrence of genetic determinants of antibiotic resistance in tap water. The aim of this study was also to compare the applied methods for antibiotic resistance genes (ARGs) investigations in tap water. As the concentration of ARGs in treated, drinking water is expected to be very low and may cause problems in a standard isolation procedure, the special emphasis is placed on the applied procedures of DNA extraction and their efficiency. The study presents the first attempts to obtain DNA directly from tap water. Further efforts must be put to determine the final amount of obtained DNA and the presence of chosen ARGs among the molecules.

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“…Las biopelículas son consideradas una barrera taxonómica para la transferencia horizontal de material genético y una fuente importante en la aparición de bacterias resistentes en el medio ambiente, la concentración de antibióticos, la alta densidad bacteriana y una larga exposición; es suficiente para promover la resistencia en bacterias (Farkas et al, 2013).…”

Section: Panorama De La Resistencia Bacteriana a Los Antibióticosunclassified

Bacterias resistentes a antibióticos en ecosistemas acuáticos

2015

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Microbiological contamination and resistance genes in biofilms occurring during the drinking water treatment process

Cited by 45 publications

References 47 publications

Widespread dissemination of class 1 integron components in soils and related ecosystems as revealed by cultivation-independent analysis

Widespread dissemination of class 1 integron components in soils and related ecosystems as revealed by cultivation-independent analysis

The occurrence of antibiotic resistance genes in tap water – a review

Bacterias resistentes a antibióticos en ecosistemas acuáticos

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