Microbiological Cutoff Values: A Critical Issue in Phenotypic Antibiotic Resistance Assessment of Lactobacilli and Bifidobacteria

Polka, Justyna Urszula; Morelli, L.; Patrone, Vania

doi:10.1089/mdr.2015.0328

Cited by 10 publications

(3 citation statements)

References 16 publications

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“…Probiotics are usually sensitive or have low resistance to antibiotics (Połka et al, 2016). The MIC of lactic acid bacteria to gentamicin is generally low because it can readily cross the cell membranes (Danielsen et al, 2004; Elkins and Mullis, 2004).…”

Section: Discussionmentioning

confidence: 99%

Laboratory Evolution Assays and Whole-Genome Sequencing for the Development and Safety Evaluation of Lactobacillus plantarum With Stable Resistance to Gentamicin

et al. 2019

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The goal of this work was to use laboratory evolution assays and whole-genome sequencing to develop and test the safety of a probiotic, Lactobacillus plantarum , with high-level of resistance to gentamicin. The evolution of L. plantarum was evaluated under the selective pressure from gentamicin and subsequently when the selective pressure was removed. After 30 days of selective pressure from gentamicin, the minimum inhibitory concentration (MIC) of L. plantarum to gentamicin increased from 4 to 512 μg/mL and remained stable at this level. After removing the selective pressure, the resistance of L. plantarum to gentamicin decreased to 64 μg/mL after 20 days, and remained stable thereafter. Although the MIC declined it was still higher than the cut-off value recommended by EFSA, indicating that the acquisition of gentamicin-resistance was an irreversible process. Using whole-genome sequencing, gene mutations were identified in the strains that had undergone selection pressure from gentamicin as well as in the strains where the selection pressure was subsequently removed. Specifically, four non-synonymous mutations were detected including one single nucleotide polymorphism (SNP), one insertion, and two structural variants (SVs), of which the mutations in genes encoding the drug resistance MFS transporter and transcriptional regulator of AraC family were only detected in the strains under selective pressure from gentamicin. The results indicate that these mutations play an important role in increasing the resistant levels of L. plantarum to gentamicin. The mobility analysis of mutant genes confirmed that they were not located on mobile elements of the genome of highly resistant L. plantarum , indicating that horizontal gene transfer was not possible.

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Section: Discussionmentioning

confidence: 99%

Laboratory Evolution Assays and Whole-Genome Sequencing for the Development and Safety Evaluation of Lactobacillus plantarum With Stable Resistance to Gentamicin

et al. 2019

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“…The level of resistance to these antibiotics exceeded a dilution of the breakpoints established by EFSA. MIC breakpoints may allow detection of some 'borderline' resistant strains (Hummel et al 2007;Połka et al 2016). This could be due to the inherent insensitivity of some of the strains to certain antibiotics due to complex intrinsic characteristics such as cell wall/membrane impermeability or metabolic properties (Hummel et al 2007;Gueimonde et al 2013).…”

Section: Evaluation Of Antibiotic Susceptibilitymentioning

confidence: 99%

Lactic acid bacteria biofilms and their ability to mitigate Escherichia coli O157:H7 surface colonization

Cisneros

Cattelan

Villalba

et al. 2021

Lett Appl Microbiol

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Significance and Impact of the Study: Nowadays, the use of LAB (lactic acid bacteria) in food-processing environments is considered as a biological strategy to control food-borne pathogens. This work provides new insights about the capacity of LAB to form biofilms and to inhibit growth and surface colonization of Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 under usual meat-processing environments. Our findings support the use of biofilm-forming LAB strains as a biological strategy to control EHEC contaminations from food-processing surfaces.

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“…From a genomic perspective, analysis of the complete genome of lactic acid‐producing bacteria is of great interest to scientists to know the pathogenicity and usefulness. To separate antibiotic susceptible strains from antibiotic‐resistant organisms, EFSA has clear guidelines for measuring the microbial cutoff values (Połka et al., 2016). The MIC distribution of the organisms can be used to determine the microbial cutoff values (Kim et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

Safety evaluation of Lactococcus lactis IDCC 2301 isolated from homemade cheese

Kim

Mondal

Jeong

et al. 2021

Food Science & Nutrition

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For applications of microorganisms as probiotics in the food industry, safety evaluation has increasingly become important to ensure the health of consumers. Although people have been using various lactic acid bacteria for different purposes, some studies have reported that certain lactic acid bacteria exhibit properties of virulence and produce toxic compounds. Thus, it is necessary to examine the characteristics associated with lactic acid bacteria that are safe for use as probiotics. This research aimed to assess the safety of Lactococcus lactis IDCC 2301 isolated from homemade cheese using in vitro and in vivo assays, including antibiotic resistance, hemolytic activity, toxin production, infectivity, and metabolic activity in immune‐compromised animal species. The results demonstrated that the strain was susceptible to nine antibiotics suggested by the European Food Safety Authority (EFSA). Whole‐genome analysis revealed that L. lactis IDCC 2301 neither has toxigenic genes nor harbors antibiotic resistance. Moreover, L. lactis IDCC 2301 showed neither hemolytic nor β‐glucuronidase activity. Furthermore, none of the D‐lactate and biogenic amines were produced by L. lactis IDCC 2301. Finally, it was demonstrated that there was no toxicity and mortality using single‐dose oral toxicity tests in rats. These results indicate that L. lactis IDCC 2301 can be safely used as probiotics for human consumption.

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Microbiological Cutoff Values: A Critical Issue in Phenotypic Antibiotic Resistance Assessment of Lactobacilli and Bifidobacteria

Cited by 10 publications

References 16 publications

Laboratory Evolution Assays and Whole-Genome Sequencing for the Development and Safety Evaluation of Lactobacillus plantarum With Stable Resistance to Gentamicin

Laboratory Evolution Assays and Whole-Genome Sequencing for the Development and Safety Evaluation of Lactobacillus plantarum With Stable Resistance to Gentamicin

Lactic acid bacteria biofilms and their ability to mitigate Escherichia coli O157:H7 surface colonization

Safety evaluation of Lactococcus lactis IDCC 2301 isolated from homemade cheese

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