Microbiological Diagnosis of the Severe Chronic Periodontitis

Abstract

In total, 14 adult patients with severe chronic periodontitis were evaluated for the presence of associated anaerobic and aerobic bacteria. Subgingival plaque specimens from three pocket depths per patient were obtained. Microaerophilic and facultative anaerobic bacteria, probably involved in the periodontitis, were isolated in six (42.9%) patients. These were Gram negative species involving Aggregatibacter (Haemophilus) aphrophilus (14.3%), Haemophilus parainfluenzae (7.1%), Kingella denitrificans (7.1%) and Moraxella osloensis (7.1%) as well as Gram-positive species, including Arcanobacterium (Actinomyces) pyogenes (7.1%) and Rhodococcus equi (7.1%). Anaerobic microbiology was completed for 12 patients. Of them, suspected periodontopathogens were isolated in seven (58.3%) patients and comprised Prevotella intermedia (in 41.7% of the patients) and Porphyromonas gingivalis (25%) as well as Porphyromonas endodontalis (8.3%). Tannerella forsythia was detected by PCR in half of the 12 cases. In conclusion, the presence of periodontopathogens as well as other bacterial species of possible importance should be considered in the patients with severe chronic periodontitis.

Prevalence of Anaerobic Bacteria in Periodontitis in Relation to Pocket Depth

“…Revealed a positive correlation between the pocket depths and the number of Gramnegative isolates (r 2 =0.716) and negative correlation (r 2 = -0.671) between pocket depth and the number of Gram-positive isolates. (15) studied the microbiological diagnosis of the sever chronic periodontitis in which anaerobic microbiology was completed for 27 patients and found that Gram-negative anaerobic bacteria were isolated in 92.6% (25 of 27 patients), involving 90.9% of untreated patients (20 of 22 cases) and all the five treated patients. Manual instrumentation decreases the population of Gram-negative bacteria and allows for an increase in the population of Gram-positive microbes.…”

The impact of the presence of Porphyromonas gingivalis on periodontal health in a group of patients with periodontitis in Erbil

“…4,5,21 Lower prevalence rate (49.1%) was reported by Boutaga et al 14 using Real-Time PCR. On studying the microbiology of subgingival plaque samples from patients with severe chronic periodontitis, Boyanova and co-workers 22 were reported a much lower detection rate (25.9%) of P. gingivalis yielded from anaerobic culturing. However, all the mentioned references agreed on that the rate of detection is higher in disease than health.…”

Subgingival Microbial Profile And Production Of Proinflammatory Cytokines In Chronic Periodontitis

“…In many studies the presence of putative periodontal pathogens in subgingival biofi lm is signifi cantly associated with ineffective response to therapy, while the lack of subgingivally established periodontal pathogens is a criterion for a good response to therapy. 3,7,10,11,19,22,23 Moreover, it is believed that the absence of pathogens in periodontal pockets is a favourable predictor of periodontal disease compared with their presence, which is considered as a disease progression predictor. 7 Authors suggest that detection of periodontal pathogens (Aa, Pg, Pi, Cr) above certain "critical" levels after active treatment show an increased risk of recurrence.…”