Microbiological investigation of samples collected from healthy middle ears during cochlear implant surgery

Kalcıoğlu, M. Tayyar; Durmaz, Rıza; Arı, Oğuz; Çelik, Serdal; Karabudak, Sema

doi:10.1016/j.diagmicrobio.2021.115390

Cited by 5 publications

(4 citation statements)

References 25 publications

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“…From these results we can speculate that the mycobiome in the middle ear is established during the first years of life and stabilized in adults. In parallel to these results, in our previous study conducted on the same samples, we found similar bacteriome composition between adults and children ( 12 ).…”

Section: Discussionsupporting

confidence: 88%

“…Previously we reported, for this same set of samples, that both healthy middle ears and ear with OME have diverse bacteriomes and viromes ( 12 , 14 , 15 ). Here, we hypothesize that the middle ear hosts a mycobiome and the composition of the mycobiome differ according to the health condition of the middle ear.…”

Section: Introductionmentioning

confidence: 84%

“…Briefly, of the 77 middle ear fluid samples, 57 were taken from the 47 individuals (20 samples were obtained from both right and left ears) with no middle ear disease and who had undergone cochlear implant surgery. Thirty-five of the 47 patients were in the child (younger than 4 years) and 12 in the adult (≥18 years) age group (Group 1) ( 12 ). Twenty middle ear samples were obtained from 20 children (aged 1.5 to 9 years) who had bilateral or unilateral persistent chronic OME for six months or longer (Group 2).…”

Section: Methodsmentioning

confidence: 99%

“…According to general opinion, immunological, anatomic, genetic, microbial, and environmental factors contribute to the development of this condition ( 10 , 11 ). Using polymerase chain reaction (PCR) and HTS-based approaches revealed a diverse bacteriome in the middle ear cavities of individuals with and without otitis media with effusion ( 12 , 13 , 14 ). There are no published data indicating fungal compositions in the middle ear cavity, which is connected to the respiratory tract.…”

Section: Introductionmentioning

confidence: 99%

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Mycobiome in the Middle Ear Cavity with and Without Otitis Media with Effusion

Durmaz¹,

Durmaz²,

Arı³

et al. 2021

TAO

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Objective: No data have yet been published revealing the composition and the diversity of fungal communities (mycobiome) in the human middle ear cavity. The presented study investigated the mycobiome in the middle ear cavities of individuals with healthy middle ears and patients with otitis media with effusion. Methods: A total of 77 middle ear and four adenoid samples were collected from 47 individuals (35 children and 12 adults) in Group 1 and from 20 children in Group 2. The mycobiome profile was analyzed with nuclear ribosomal internal transcribed spacer 2 (ITS2) based metabarcoding using an Illumina MiSeq metagenomics kit. Results: ITS2-based metabarcoding detected 14 different genera and 17 different species with a mean relative abundance of ≥1% in the samples analyzed. Mycobiome profile was similar between the adenoid tissue and the middle ear cavity, between Groups 1 and Group 2, and between children and adults. Fusarium, Stemphylium, Candida , and Cladosporium were the most abundant genera detected in all samples. The mean relative abundances of the genera Candida and Fusarium were remarkably higher in Group 2 compared to Group 1. Conclusion: The species Candida glaebosa, Candida cretensis, Aspergillus ruber, Penicillium desertorum , and Rhizopus arrhizus were significantly more abundant in patients with otitis media with effusion (OME), raising the possibility that they affect the pathogenesis of OME.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 84%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mycobiome in the Middle Ear Cavity with and Without Otitis Media with Effusion

Durmaz¹,

Durmaz²,

Arı³

et al. 2021

TAO

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Respiratory viruses in the healthy middle ear and middle ear with otitis media with effusion

Durmaz

Abdulmajed

Durmaz

et al. 2021

Journal of Medical Virology

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To investigate the presence of respiratory viruses in the middle ear cavity of the individuals with a healthy middle ear and the children with otitis media with effusion (OME). A total of 72 middle ear samples were collected from 25 children with OME (Group 1) and 47 individuals with no middle ear disease (Group 2). Multiplex realtime polymerase chain reaction was used to investigate the presence of 20 different respiratory viruses. Virus results were compared with bacteriomes of the same populations. At least one respiratory virus was detected in 56% of the patients in Group 1 and 12.8% of the individuals in Group 2. The viral co-infection rate for Group 1 and 2 was 8% and 2.1%, respectively. In Group 1, adenovirus was the most frequently detected virus with a rate of 24%, either alone (16%) or concurrent with other viruses (8%), followed by influenza B (12%), rhinovirus, and bocavirus (8%) each. Parainfluenza 4, coronavirus OC43, and RSV A/B were detected in 4% of the sample each. In Group 2, rhinovirus was detected in two samples (4.3%) followed by adenovirus, coronavirus OC43, coronavirus E299, and coronavirus NL63 with a rate of 2.1% each. The detection rate of respiratory viruses was significantly higher in children aged 6 to 11 years. There was no positive association between virus and bacteria found in the middle ear cavity. The current study has provided comprehensive data indicating the presence of diverse respiratory viruses in the healthy middle ear cavity. Our results also suggest that respiratory viruses might have a contribution to OME pathogenesis.

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