Microbiological profile of root canals indicated for endodontic retreatment due to secondary endodontic infections or for prosthetic reasons

Godoi-Jr, Ederaldo P; Bronzato, Juliana Delatorre; Francisco, Priscila Amanda; Bícego-Pereira, Eloá Cristina; Lopes, Érica Mendes; Passini, Maicon Ricardo Zieberg; Soares, Adriana de Jesus; Almeida, José Flávio Affonso de; Marciano, Marina Angélica; Ferraz, Caio Cézar Randi; Gomes, Brenda P. F. A.

doi:10.1007/s00784-023-04947-x

Cited by 3 publications

(1 citation statement)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All groups exhibited an increase in the relative abundance of Firmicutes, in particular Staphylococcus, Streptococcus, and Gemella. These bacterial genera have been previously identified in root canal retreatment cases (Barbosa-Ribeiro et al, 2021;Godoi-Jr et al, 2023). Furthermore, a study by Fouad et al (2022) documented the persistence of Firmicutes in the canals of teeth undergoing revitalization procedures.…”

Section: Discussionmentioning

confidence: 83%

Effect of supplementary sodium hypochlorite agitation techniques on an ex vivo oral multispecies biofilm during passive disinfection of simulated immature roots

Vieira,

de‐Jesus‐Soares,

Lopes

et al. 2024

Int Endodontic J

Self Cite

View full text Add to dashboard Cite

AimTo compare the effect of different sodium hypochlorite (NaOCl) agitation techniques on an ex vivo oral multispecies biofilm during passive disinfection of simulated immature roots.MethodologyExtracted human teeth were prepared to simulate immature roots. They were infected with a dental plaque‐derived multispecies biofilm and cultured for 14 days. The roots were randomly designated into four groups: (1) negative control (PBS), (2) 1.5% NaOCl (CNI), (3) CNI + Ultrasonic activation (UA), (4) CNI + EasyClean agitation (ECA), (5) CNI + XP‐endo finisher agitation (XPF), and (6) positive control (6% NaOCl). Biofilm samples were collected from the root canals and used to determine the number of viable cells (colony‐forming units), scanning electron microscopy, and 16S rRNA gene sequencing. The mean colony‐forming units per mL (CFU/mL) were analysed using One‐way anova. 16S rRNA sequencing data were analysed for alpha (observed OTUs, Shannon index, and Chao1) and beta diversity (Bray‐Curtis dissimilarities). The LEfSe analysis was used to determine the effect of treatment procedures on the abundance of root canal microbiota. The significance was set at .05.ResultsPBS and CNI samples had significantly higher CFU/mL counts than UA, ECA, XPF, and 6% NaOCl samples (p < .05). The pre‐treatment, PBS, and CNI groups had significantly greater alpha diversity than the UA, ECA, XPF, and 6% NaOCl groups (p < .05). NaOCl agitation groups and the 6% NaOCl group achieved a more pronounced reduction in bacteria from the genera Fusobacterium, Actinomyces, Porphyromonas, and Capnocytophaga.ConclusionsThe effectiveness of passive disinfection protocols was enhanced by NaOCl agitation techniques, suggesting that this supplementary method can improve the outcome of revitalization procedures.

show abstract

Section: Discussionmentioning

confidence: 83%