Microbiological profile of symptomatic and asymptomatic bacteriuria recovered from female patients with urinary tract infection

Abstract

With the previous knowledge on the production of ?-lactamase by the bacterial pathogens causing urinary tract infection, present study further investigated the presence of symptomatic and asymptomatic bacteriuria in female patients admitted into the Delta Medical College with suspected urinary tract infections (UTIs). The manifestation of uropathogens and their responses against locally available antibiotics (amoxicillin, 10 ?g; cephradin, 30 ?g; ciprofloxacin, 5 ?g; cotrimoxazole, 23.8 ?g; gentamicin, 10 ?g; nalidixic acid, 30 ?g) were inquired by means of conventional cultural techniques and double-disc diffusion methods, respectively. Among 110 urine samples collected from the patients with suspected UTI, 34 were found to be culture positive. Symptomatic (64.7%) and asymptomatic (36.3%) bacteriuria were noticed inside the puss cell of the UTI positive cases. Escherichia coli (73.5%) was the dominant bacteria while Klebsiella spp. (26.5%) was also exultant. Around 96% uropathogens were found to be sensitive against imipenem, and 75% against amikacin. E. coli was found to be sensitive against all of the antibiotics used, whereas Klebsiella spp. was found to be 100% resistant against nalidixic acid and cotrimoxazole. DOI: http://dx.doi.org/10.3329/sjm.v3i1.22751 Stamford Journal of Microbiology, Vol.3(1) 2013: 34-37

Pathogenic identification and antibiotic susceptibility of the microorganisms isolated from urine samples of UTI patients

“…Urine samples were collected aseptically from the mid stream in the sterile bottles provided by the test centers and immediately transferred to different culture media. Standard laboratory procedures were strictly maintained to avoid any hazards and transmissions to healthcare workers and to others (14)(15)(16).…”

“…After collection of urine samples, they were inoculated onto MacConkey agar plates and CLED (cysteine-, lactose-and electrolyte-deficient) agar plates to detect mostly Gram negative bacteria (14)(15)(16)). An aliquot of 10µl sample was spread over both the types of agar plates and incubated at 37 o C for 24 hours.…”

“…Pathogenic bacteria a found in the urine samples were further subjected to determine their antibiotic sensitivity patterns against the commonly prescribed antibiotics (14)(15)(16)21). About 11 antibiotics such as gentamicin (GEN, 10µg), cephradine (CED, 30µg), cefuroxime (CXM, 30µg), ceftadizime (CAZ, 30µg), amikacin (AMK, 10µg), nitrofurantoin (NIT, 300µg), ciprofloxacin (CIP, 05 µg), levofloxacin (LE, 5 µg), rifampin (RIF, 05 µg), ceftriaxone (CTR, 30 µg), cotrimoxazole (COT, 25 µg) were selected to be tested for this study.…”

“…etc (7,9,10). Despite of many antibacterial mechanisms in the urinary bladder to have infection, the uropathogenic bacteria can invade such mechanisms and colonize in the urinary tract to start an infection (11)(12)(13)(14)(15). Different tests are in use to detect UTI such as urinalysis, Gram staining, urine culture and so on (15).…”

“…The physician prescribes the susceptible antibiotics from the result chart but the condition gets worse when patients use uncontrolled dosage without becoming aware of the drug resistant threats (14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27). a Immuno-compromised patients are at a great risk of UTI and the drug resistant traits of the pathogens makes the phenomenon even more complicated.…”