Microbiological Quality And Safety Of <i>Rastrineobola Argentea</i> Retailed In Kisumu Town Markets, Kenya

Abstract

Objective: To investigate faecal contamination and safety of Rastrineobola argentea sold in retail markets in Kisumu town. Design: This was a repeated cross sectional study and based on random sampling. Setting: Kisumu city, targeting six markets; Oile, Jubilee, Kibuye, Kondele, Nyalenda and Manyatta. Results: A total of 60 fish samples were analysed. All the fish were found to be contaminated with E. coli, and in addition 6.67% of the fish products tested positive for Salmonella. Shigella was absent in all samples analysed. 26.53% of E. coli isolates tested were resistant to two or more antimicrobial agents tested, with the highest level of resistance detected against cotrimoxazole at 38.76%. The E. coli multiple antibiotic resistance (MAR) index was 0.084 indicating that the contamination was not originating from a high -risk source. A plasmid of approximately 5.6 kb was commonly isolated from E. coli isolates that showed resistance to ampicillin. Plasmids isolated were not transferable by conjugation. Conclusion: The presence of Salmonella spp and occurrence of MDR E. coli were identified as some of the possible health risks that may be associated with R. argentea displayed for sale in Kisumu city markets. This possess a real health risk through consumption or directly through contact with the fish products.

Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil

“…These findings of antimicrobial susceptibility are in agreement with data from previous studies, which found that resistance to aminoglycosides, β -lactamase, and penicillin is common among E. coli isolates from food of animal origin [ 47 – 49 ]. However, the resistance frequency in E. coli isolated in the present study was low when compared to other studies, where a resistance of 58% and 42% in raw fish samples from Kenya and Vietnam, respectively, was observed [ 48 , 50 ]. Mussel samples from Niterói (Brazilian Southeastern oceanic region) showed 29% resistance to at least one antimicrobial [ 31 ], and strains isolated from mussels from the Guanabara Bay, Rio de Janeiro, showed 40% to 85% resistance to tested antimicrobials [ 51 ], indicating the intense presence of domestic and industrial effluents.…”

Prevalence and Antimicrobial Resistance of Bacteria Isolated from Marine and Freshwater Fish in Tanzania

“…is is in contrast to the results obtained by Kibret and Abera [39], where erythromycin and tetracycline were highly resistant to E. coli isolates at 89.4 and 72.6%, respectively. e high prevalence of resistance to tetracycline, ampicillin, and cotrimoxazole in E. coli in the region has also been reported by Sifuna et al [40], in which E. coli demonstrated resistance mostly to ampicillin and tetracycline. According to Sifuna et al [40], the resistance pattern reported in their study can also be linked to the use of these drugs in veterinary practice and lead to resistance in humans.…”

Detection of Virulence Gene and Antimicrobial Resistance Pattern of Escherichia coli Isolated from Fresh Water Fish in and around Anand City, Gujarat, India

“…The antibiogram of all isolates revealed high degree of sensitivity to Chloramphenicol (95.34%) and gentamicin (93.02%) while high frequency of resistance were observed to ampicillin (67.44%) and streptomycin (32.35%). Results revealed that 67.44 per cent of the isolates showed resistance to ampicillin and such a high percent of resistance among the isolates is in agreement with the findings of Ogbonna et al, (2008), Sifuna et al, (2008), Bolarinwa et al, (2011) and Sanyal et al, (2011) but in contrast to this Barbosa (2014) reported all isolates were sensitivity to ampicillin. Higher frequency of resistance to streptomycin (32.35%) was observed during present study.…”