Microbiological quality of marketed fresh and frozen seafood caught off the Adriatic coast of Croatia

Abstract

Fresh and frozen seafood products (fish, shellfish, crustaceans, molluscs) in wide use in Croatia and typical of the Mediterranean diet, were examined for the presence of microbiological contamination through the winter and summer seasons. Total bacterial counts of aerobic mesophilic bacteria (AB), aerobic psychrophilic bacteria (AP), Salmonella spp., Enterobacteriaceae, Escherichia coli, Staphylococcus aureus, sulphite-reducing clostridia (SRC), Listeria monocytogenes, Vibrio cholerae and V. parahaemolyticus were measured. The microbiological quality of individual samples varied widely between animal species and also between winter/summer seasons regarding total counts of aerobic mesophilic and psychrophilic bacteria. The poorest quality was for (both summer and winter) fish samples, where 66.6 % of fresh and frozen fish were found unacceptable by Croatian standards. The overall prevalence of V. parahaemolyticus was 5%. Its recovery rate was higher in fresh/ frozen shellfish in both seasons than in other specimens or other storage/season conditions. Fresh crustaceans sampled in winter demonstrated significantly higher aerobic mesophilic counts than frozen ones. Unacceptable Enterobacteriaceae levels were obtained in 40% of the fresh fish summer samples. The results of this survey constitute an indicator of bacteriological contamination of a variety of seafood. The findings could serve as a basis for future testing of seafood, and possibly as a template for developing a regional/Mediterranean testing scheme on the microbial contamination of seafood in order to establish data with comparative epidemiological and statistical values.

Quality and Shelf-Life Modeling of Frozen Fish at Constant and Variable Temperature Conditions

“…Total viable count in frozen gilthead sea bream fillets, European sea bass fillets and yellowfin tuna slices was 4.4 ± 0.2, 5.5 ± 0.3 and 3.5 ± 0.2 logCFU/g, respectively. These values are in accordance with the respective microbial count levels reported in the literature for frozen fish [ 33 , 37 ]. No significant growth was observed during the storage of frozen fish samples at any of tested temperatures.…”

ISOLATION OF Salmonella spp. AND OTHER MEMBERS OF Enterobacteriaceae FROM HORSE MACKEREL (Tra-churus trachurus), SOLD IN PUBLIC MARKETS OF ISTANBUL, TURKEY

“…The numbers and percentages of bacterial species, isolated from horse mackerels during in spring and autumn were shown in Table 3. These organisms were isolated from shrimp in India (Jonnalagadda and Bhat, 2004), seafoods in Greece (Papadopoulou et al, 2007), fresh and frozen seafoods in Croatia (Topic Popovic et al, 2010), fresh fish in Sudan (Goja, 2013), similarly. Some of the potential pathogenic microorganisms (Citrobacter spp., P. mirabilis and P. vulgaris) were isolated during spring season.…”

“…Prevalence of Salmonella and some bacterial species, belonging to the Enterobacteriaceae family, have been studied in different regions of the world and health risks were evaluated. Microbiological quality of seafood in Croatia (Topic Popovic et al, 2010), fish in Khartoum, Sudan (Yagoub, 2009), fish and crustaceans in Coimbatore, India (Hatha and Lakshmanaperumalsamy 1997), shrimp in India (Hatha et al, 1998;Jonnalagadda and Bhat, 2004), seafood in Greece (Papadopoulou et al, 2007), cooked shellfish in UK (Sagoo et al, 2007), hygiene conditions of wholesale fishmarket in Istanbul (Ucok, 2003), microbiological quality of stuffed mussel in Istanbul (Bingol et. al., 2008), determinetion of pathogen microorganisms in seafood in Istanbul (Ucok Alakavuk, 2009) and quality of fish from retail markets in Istanbul (Mol and Tosun 2011), were studied.…”

Veterinary and sanitary evaluation of frozen fish imported into Ukraine for presence of antibacterial substances residues

“…Значне і необмежене застосування антибактеріальних препаратів призводить до накопичення їх у водному середовищі, тканинах риби і відповідно в рибних продуктах (Samanidou and Evaggelopoulou, 2007;Akinbowale et al, 2007;Rico et al, 2012;Zhang et al, 2013;Bayer et al 2017). Так, дослідники (Topic Popovic et al, 2010;Chen, 2014;Bayer et al, 2017) виявляли антибактеріальні препарати різних груп у рибі в Україні, Китаї, Хорватії, Австралії. У дослідженнях, проведених в (Bayer et al, 2017) виявляли залишки ветеринарних препаратів нітрофуранового ряду, сульфаніламіди, антибіотики у рибі в різних регіонах України.…”