Microbiological safety and antibiotic resistance risks at a sustainable farm under large-scale open-air composting and composting toilet systems

Liu, Ning; Xu, Like; Han, Lujia; Huang, Guangqun; Ciric, Lena

doi:10.1016/j.jhazmat.2020.123391

Cited by 29 publications

(6 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Where feasible, the installation of barn flooring that could be subject to frequent runoff should be considered, along with the implementation of proper drainage systems. Finally, it is important to improve the monitoring of microbiologically safe biofertilizers through suitable indicators and to promote the use of safer composting methods, as previously discussed by other authors [62][63][64][65].…”

Section: Discussionmentioning

confidence: 99%

Physicochemical and Microbial Properties of Dairy Barn Soils: A Case Study in Costa Rican Farm-Associated Soils Harboring the Foodborne Pathogen Listeria monocytogenes

Guillén-Watson,

Barboza-Fallas,

Masís-Meléndez

et al. 2023

Sustainability

View full text Add to dashboard Cite

Animal farming activities can influence soil properties that contribute to the survival of foodborne pathogens like the ubiquitous Listeria monocytogenes. However, the presence of this pathogen in farm-associated soils in Costa Rica has not been studied, which might provide new insights regarding the environmental conditions associated with the establishment of this pathogen. In this work, we correlated the presence of L. monocytogenes with the soil physicochemical properties and bacterial community structure of soils associated with livestock activities, including a dairy barn floor (DB) and a slaughterhouse holding pen (SH). A cropland (CL) was included as an example of soil not associated with animal farming practices. We characterized the presence pattern of L. monocytogenes via culture-dependent and culture-independent techniques (i.e., metabarcoding based on 16S rRNA gene sequencing) and conducted a determination of physical, elemental and chemical parameters with Fourier-transform infrared spectroscopy (FT-IR) to statistically determine the soil properties that correlate with L. monocytogenes’ presence in the soil. L. monocytogenes was isolated from DB samples and SH but not from CL. Subsequently, 16S rRNA gene-based metabarcoding showed that the presence of L. monocytogenes was positively correlated with higher bacterial diversity, while physicochemical analyses revealed that the total hydrogen and nitrogen contents of soil organic matter, pH, and electrical conductivity were the main drivers of L. monocytogenes’ presence. Moreover, a CL sample fertilized with animal-derived products showed DB-like physicochemical properties matching conditions in favor of L. monocytogenes’ presence. Hence, our work emphasizes the significance of soil as a primary source for the widespread dissemination of pathogens, particularly underscoring the necessity for improved agricultural practices to prevent cross-contamination with L. monocytogenes. Additionally, we highlight the importance of further understanding the biotic and abiotic factors in facilitating the establishment of L. monocytogenes.

show abstract

Section: Discussionmentioning

confidence: 99%

Physicochemical and Microbial Properties of Dairy Barn Soils: A Case Study in Costa Rican Farm-Associated Soils Harboring the Foodborne Pathogen Listeria monocytogenes

Guillén-Watson,

Barboza-Fallas,

Masís-Meléndez

et al. 2023

Sustainability

View full text Add to dashboard Cite

show abstract

“…In fact, amoxicillin was the least effective antibiotic since 11 of the 19 strains showed resistance to this compound. Liu et al (2021) also reported the presence of microorganisms isolated of manures resistant to this antibiotic. The extensive use of amoxicillin in both humans and animals has increased resistance to this compound (Sodhi et al, 2021).…”

Section: Antibiotic Resistancementioning

confidence: 94%

Industrial Composting of Sewage Sludge: Study of the Bacteriome, Sanitation, and Antibiotic-Resistant Strains

et al. 2021

View full text Add to dashboard Cite

Wastewater treatment generates a huge amount of sewage sludge, which is a source of environmental pollution. Among the alternatives for the management of this waste, industrial composting stands out as one of the most relevant. The objective of this study was to analyze the bacterial population linked to this process and to determine its effectiveness for the reduction, and even elimination, of microorganisms and pathogens present in these organic wastes. For this purpose, the bacteriome and the fecal bacteria contamination of samples from different sewage sludge industrial composting facilities were evaluated. In addition, fecal bacteria indicators and pathogens, such as Salmonella, were isolated from samples collected at key stages of the process and characterized for antibiotic resistance to macrolide, β-lactam, quinolone, and aminoglycoside families. 16S rRNA phylogeny data revealed that the process clearly evolved toward a prevalence of Firmicutes and Actinobacteria phyla, removing the fecal load. Moreover, antibiotic-resistant microorganisms present in the raw materials were reduced, since these were isolated only in the bio-oxidative phase. Therefore, industrial composting of sewage sludge results in a bio-safe final product suitable for use in a variety of applications.

show abstract

“…According to the maximum value of the bacterial Minimum Inhibition Concentration, AMX-resistant bacteria and TCB were counted on R2A medium with and without 32 mg/L AMX by referring to previous experience ( Liu et al, 2020 ). The colony formation unit (CFU) per gram was calculated by the formula (1) .…”

Section: Methodsmentioning

confidence: 99%

Environmental drivers and interaction mechanisms of heavy metal and antibiotic resistome exposed to amoxicillin during aerobic composting

Liu

Li²,

Su³

et al. 2023

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

The environmental accumulation and spread of antibiotic resistance pose a major threat to global health. Aerobic composting has become an important hotspot of combined pollution [e.g., antibiotic resistance genes (ARGs) and heavy metals (HMs)] in the process of centralized treatment and resource utilization of manure. However, the interaction mechanisms and environmental drivers of HMs resistome (MRGs), antibiotic resistance (genotype and phenotype), and microbiome during aerobic composting under the widely used amoxicillin (AMX) selection pressure are still poorly understood. Here, we investigated the dynamics of HMs bioavailability and their MRGs, AMX-resistant bacteria (ARB) and antibiotic resistome (ARGs and intI1), and bacterial community to decipher the impact mechanism of AMX by conducting aerobic composting experiments. We detected higher exchangeable HMs and MRGs in the AMX group than the control group, especially for the czrC gene, indicating that AMX exposure may inhibit HMs passivation and promote some MRGs. The presence of AMX significantly altered bacterial community composition and AMX-resistant and -sensitive bacterial structures, elevating antibiotic resistome and its potential transmission risks, in which the proportions of ARB and intI1 were greatly increased to 148- and 11.6-fold compared to the control group. Proteobacteria and Actinobacteria were significant biomarkers of AMX exposure and may be critical in promoting bacterial resistance development. S0134_terrestrial_group was significantly negatively correlated with blaTEM and czrC genes, which might play a role in the elimination of some ARGs and MRGs. Except for the basic physicochemical (MC, C/N, and pH) and nutritional indicators (NO3−-N, NH4+-N), Bio-Cu may be an important environmental driver regulating bacterial resistance during composting. These findings suggested the importance of the interaction mechanism of combined pollution and its synergistic treatment during aerobic composting need to be emphasized.

show abstract

Microbiological safety and antibiotic resistance risks at a sustainable farm under large-scale open-air composting and composting toilet systems

Cited by 29 publications

References 61 publications

Physicochemical and Microbial Properties of Dairy Barn Soils: A Case Study in Costa Rican Farm-Associated Soils Harboring the Foodborne Pathogen Listeria monocytogenes

Physicochemical and Microbial Properties of Dairy Barn Soils: A Case Study in Costa Rican Farm-Associated Soils Harboring the Foodborne Pathogen Listeria monocytogenes

Industrial Composting of Sewage Sludge: Study of the Bacteriome, Sanitation, and Antibiotic-Resistant Strains

Environmental drivers and interaction mechanisms of heavy metal and antibiotic resistome exposed to amoxicillin during aerobic composting

Contact Info

Product

Resources

About