A total of 90 random samples of raw cow's milk used in small scale ice cream , small scale ice cream and large scale plain ice cream(30 samples each) collected from Assiut city, Egypt , in the period between from April and September 2019. Each sample was put in ice tank with thermometer to maintain temp. at 4C. The samples were dispatched to the laboratory with a minimum of delay where they were examined for coliforms, fecal coliforms and E.coli true faecal type. The results show that the mean counts of total coliforms in raw cow's milk, small scale and large scale plain ice cream were 2.4x10 3 , 2.8x10 2 and 1.56x10 2 cfu/ml or g. , respectively. While the mean counts of fecal coliforms were 2.1x10 3 , 1.9x10 2 and 1.32 x10 2 cfu/ml or g. , respectively and the mean counts of E.coli true faecal type count cfu/ml or g. were 2.5x10 2 , 3.3x10 2 and 1.12x10 2 cfu/mlor g., respectively. The heath hazards of coliforms, fecal coliforms and E.coli true faecal type, methods of contamination and control were discussed.