Microbiological stability of biodiesel–diesel-mixtures

Schleicher, T.; Werkmeister, R.; Ruß, Winfried; Meyer-Pittroff, R.

doi:10.1016/j.biortech.2008.07.029

Cited by 100 publications

(76 citation statements)

References 9 publications

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“…After 16 weeks elapsed time, the microbial growth in the ULSD blends has escalated ranging from 10 4 -10 6 cfu ml -1 In the B20 ULSD sample the detected contamination was heavier compared to the B5 and B10 counterparts. Similar observations have been reported in the literature (Dodos, 2009;Schleicher et al, 2009;Sorensen et al, 2011) and it could be reckoned that these results are indicative of the negative effect that the higher concentration of FAMEs has on the microbial stability of the diesel/biodiesel blends. On the other hand it is worth noticing that according to the results obtained from the CFU tests the microbial growth in the LSD biodiesel blends was still inhibited after 16 weeks of incubation, with the exception of the B5 sample which demonstrated a small amount of contamination.…”

Section: Microbial Growthsupporting

confidence: 90%

“…Its chemical composition along with its hydroscopic nature makes it more "biologically active" and as a result the final blends could be more prone to microbiological contamination. (Dodos et al, 2009;Schleicher et al, 2009;Sorensen et al, 2011). On the other hand it is suggested that the decrease of sulfur content in automotive diesel may also play an important role to the promotion of microbial growth.…”

Section: Introductionmentioning

confidence: 99%

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Effects of microbiological contamination in the quality of biodiesel fuels

Dodos

Konstantakos²,

Loginos³

et al. 2013

Global NEST Journal

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The introduction to the European Market of biodiesel blends along with the minimization of the sulphur content in automotive diesel has rejuvenated the research interest on the microbial stability of diesel fuel. Several microorganisms are able to metabolize hydrocarbons contained in conventional fuels and particularly in diesel and jet stocks. With the advent of FAME (Fatty Acid Methyl Esters) as a diesel fuel substitute there has been an increase in the number of samples suspect of microbial contamination with confirmative results. The aim of this study was to investigate the microbiological stability of FAME/diesel blends and consequently the impact of microbial proliferation on their quality. A commercially available FAME was blended with Ultra-Low Sulphur (ULSD) and Low Sulphur (LSD) conventional automotive diesel fuels in mixing ratios of 5, 10 and 20 % v/v. The resulting blends were contaminated with bottom-water of known viable microbial colonies and were stored for a period of 16 weeks. During storage the microbiological growth was evaluated by employing both semi-quantitative and quantitative methodologies. At the same time the devolution of certain quality parameters, namely oxidation stability and acid number, which could be influenced by microbial growth was examined. The overall results reveal the need to establish a scheduled inspection plan adapted to the diesel fuel supply chain infrastructure aiming to control and remedy efficiently the microbiological growth issues.

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Section: Microbial Growthsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Effects of microbiological contamination in the quality of biodiesel fuels

Dodos

Konstantakos²,

Loginos³

et al. 2013

Global NEST Journal

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“…Toxicological evaluation during the biodegradation process which alternative fuels are used in road traffic in order to reduce dependence on crude oil imports, reach the goals of the Kyoto Protocol, and support the agriculture sector (19).…”

Section: Tamada Is E T a Lmentioning

confidence: 99%

“…However, this alternative only gained focus after the petroleum crisis in the 1970s. Currently, with the growing environmental concern for the contamination and degradation of ecosystems, energy sources with more ecological appeal emerge as alternatives to nonrenewable resources with higher pollution potential (19).…”

Section: Introductionmentioning

confidence: 99%

Toxicological evaluation of vegetable oils and biodiesel in soil during the biodegradation process

Tamada¹,

Montagnolli²,

Lopes³

et al. 2012

Braz. J. Microbiol.

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Vegetable oils and their derivatives, like biodiesel, are used extensively throughout the world, thus posing an environmental risk when disposed. Toxicity testing using test organisms shows how these residues affect ecosystems. Toxicity tests using earthworms (Eisenia foetida) are widespread because they are a practical resource for analyzing terrestrial organisms. For phytotoxicological analysis, we used seeds of arugula zero days, 60 days, 120 days and 180 days. The studied contaminants were soybean oil (new and used) and biodiesel (B100). An evaluation of the germination of both seeds showed an increased toxicity for all contaminants as the biodegradation occurred, biodiesel being the most toxic among the contaminants. On the other hand, for the tests using earthworms, the biodiesel was the only contaminant that proved to be toxic. Therefore, the higher toxicity of the sample containing these hydrocarbons over time can be attributed to the secondary compounds formed by microbial action. Thus, we conclude that the biodegradation in soil of the studied compounds requires longer periods for the sample toxicity to be decreased with the action of microorganisms.

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“…Em meio aquático, o biodiesel teve um efeito negativo no processo de biodegradação dos compostos BTEX, pois os hidrocarbonetos monoaromáticos foram mais lentamente degradados na presença do biocombustível do que em sua ausência (CORSEUIL et al, 2011a). Contudo, há indí-cios de que o aumento da biomassa degradadora é estimulado na presença de biodiesel (SCHLEICHER et al, 2009;OWSIANIAK et al, 2009) e, assim, os hidrocarbonetos podem ser rapidamente consumidos após a biodegradação do biodiesel.…”

Section: Introductionunclassified

Influência do biodiesel de soja na biodegradação anaeróbia do benzeno e tolueno

Schambeck

Ramos

Chiaranda

et al. 2015

Eng. Sanit. Ambient.

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A influência do biodiesel de soja na biodegradação dos hidrocarbonetos monoaromáticos benzeno e tolueno foi estudada sob condições anaeróbias em dois microcosmos montados com água subterrânea sintética, inóculo metanogênico, benzeno, tolueno e biodiesel. Na presença de biodiesel não foi observada biodegradação do benzeno e do tolueno. Com a biodegradação do biodiesel ocorreu a formação de acetato e metano, uso do sulfato e um aumento de 45 vezes no número de bactérias sulfato-redutoras.Esses resultados mostraram que, na mistura com benzeno e tolueno, o biodiesel foi biodegradado tanto sob condições de sulfato-redução quanto metanogênicas e que sua presença estimulou o crescimento da biomassa. Palavras

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Microbiological stability of biodiesel–diesel-mixtures

Cited by 100 publications

References 9 publications

Effects of microbiological contamination in the quality of biodiesel fuels

Effects of microbiological contamination in the quality of biodiesel fuels

Toxicological evaluation of vegetable oils and biodiesel in soil during the biodegradation process

Influência do biodiesel de soja na biodegradação anaeróbia do benzeno e tolueno

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