Microbiological Synthesis of C<sup>14</sup>-Labeled Streptomycin

Karow, Edward O.; Peck, Robert L.; Rosenblum, Charles; Woodbury, David T.

doi:10.1021/ja01132a030

Cited by 19 publications

(7 citation statements)

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“…These investigators administered D-glucose-U-14C to S. griseus grown on a soybean-distillers solubles-glucose medium. Despite the presence of available carbon from glucose and other carbon compounds in the complex culture medium, a 5 % incorporation of the labeled D-glucosewas achieved in contrast to the approximately 1 % incorporation formerly obtained (KAROW et al, 1952). This increased incorporation was due to the 60 hour delay, until mycelial mass was maximal, for the addition of radioactive glucose to the final production medium; this is in contrast to the earlier work where 14C glucose was added initially.…”

Section: Glucose the Precursor Of The Carbon Backbone Of Streptomycincontrasting

confidence: 69%

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Biosynthesis

1967

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All rights, especially that of translation into f oreign 1anguages, reserved. It is also forbidden to reproduce this book, either who1e or in part, by photomechanical means (photostat, microfilm and/or microcard) or by other procedure without written pennission from Springcr-Vcrlag Berlin Heide1bCig GmbH. DedicationFor most areas of scientific pursuit, there is usually that rare investigator who has the imagination to conceive ideas, who has faith in his visions, and who has the ability to critically test his concepts in the laboratory. Almost invariably, this scientist also inspires younger men to enthusiastically enter into his research program. To him should go the accolades and the recognitions of the esteem in which he is held. As only a small part of this esteem, we wish to dedicate these books to Professor SELMAN A. W AKSMAN in appreciation of his leadership and contributions in all facets of antibiotic research. PrefaceThe idea for publishing these books on the mechanism of action and on the biosynthesis of antibiotics was born of frustration in our attempts to keep abreast of the literature. Gone were the years when we were able to keep a bibliography on antibiotics and feel confident that we could find everything that was being published on this sUbject. These fields of investigation were moving forward so rapidly and were encompassing so wide a range of specialized areas in microbiology and chemistry that it was almost impossible to keep abreast of developments. In our naivete and enthusiasm, however, we were unaware that we were toying with an idea that might enmesh us, that we were creating an entity with a life of its own, that we were letting loose a Golom who instead of being our servant would be our master.That we set up ideals for these books is obvious; they would be current guides to developments and information in the areas of mechanism of action and biosynthesis of antibiotics. For almost every subject, we wished to enlist the aid of an investigator who himself had played a part in determining the nature of the phenomena that were being discussed. One concept for the books was that they include only antibiotics for which a definitive, well-documented mechanism of action or biosynthetic pathway was known. Yet, such an approach would not entirely serve the purpose we projected, for it would not encompass all of the information available in these fields of antibiotic investigations and blind searches for the original literature would still have to be made. We therefore chose to include any and all antibiotics about which some pertinent information had been published. It was obvious even at the start that such a compilation, integration, and analysis of information could never be complete unless scientific investigations ceased at the moment the last manuscript was submitted-an end that was neither desirable nor possible. An addendum was therefore included at the end of the volume and left open for the addition of new information until the last pages of the regular articles had been printed.The...

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Section: Glucose the Precursor Of The Carbon Backbone Of Streptomycincontrasting

confidence: 69%

“…The earliest experiment employing isotopes was performed by KAROW et al (1952). D-Glucose-U-14Cwas given to S.griseus grown on a complex organic medium.…”

Section: Glucose the Precursor Of The Carbon Backbone Of Streptomycinmentioning

confidence: 99%

Biosynthesis

1967

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“…As these carbon atoms arise largely, if not entirely, from carbon dioxide (Hunter, Herbert & Hockenhull, 1954), atoms from the substrate are presumably incorporated only after these have been completely metabolized by the organism. Karow, Peck, Rosenblum & Woodbury (1952) have demonstrated the incorporation of the labelled carbon of [14C]glucose into streptomycin. They did not, however, submit their product to chemical degradation to determine the distribution of the radioactivity within the molecule.…”

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confidence: 99%

Actinomycete metabolism. Incorporation of 14C-labelled compounds into streptomycin

Hunter¹,

Hockenhull²

1955

Biochemical Journal

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“…KAROW et al (1952) ftrst reported the preparation of 14C_la_ beled streptomycin by feeding labeled glucose or starch to the growing culture of S.griseus, then HUNTER and HOCKENHULL (1955) found that about 5% of D-[14C]glucose supplied to a 60-h culture of S. griseus was incorporated into streptomycin with the radioactivity equally distributed into the three subunits (streptidine, 34.1 %; streptose, 31.7%; and N-methyl-L-glucosamine, 34.8%). Thereafter, 14C-labeled compounds were used extensively as discussed in review articles by HOCKENHULL (1960), MENDICINO and PICKEN (1966), HORNER (1967), AKAMATSU (1968), andINAMINE (1970).…”

Section: Streptomycin and Bluensomycinmentioning

confidence: 99%