Microbiological Turbidimetric Methods: Linearization of Antibiotic and Vitamin Standard Curves

Kavanagh, Frederick

doi:10.1002/jps.2600661104

Cited by 5 publications

(2 citation statements)

References 11 publications

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“…Generally, dose-response relations are not a straight line, but linearity can be achieved through transformation [43]. One of the most commonly used methods for transformations is the logarithmic transformation [44].…”

Section: Validity Of the Linearity Curvementioning

confidence: 99%

Microbiological Antibiotic Assay Validation of Gentamicin Sulfate Using Two-Dose Parallel Line Model (PLM)

Eissa

Rashed²,

Eissa³

2021

HighTech. Innov. J.

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Till nowadays microbiological assay is still widely used with several antibiotics that are composed of a mixture of related active compounds. However, obtaining a reasonably valid determination of the potency is dependent on the validity and the suitability of the assay design. The present work aimed to validate an assay design of an aminoglycoside antibiotic (Gentamicin Sulfate) using a two-dose Parallel Line Model agar diffusion assay in a large 8×8 rectangular plate. All preparatory procedures were done following United States Pharmacopeia and the Inhibition Zones were measured using a digital caliper to the nearest 0.01 mm. Analysis of variance of compendial requirements of regression and parallelism were found to be satisfactorily meeting the acceptance criteria. Specificity was achieved for the product under investigation with no detectable IZ that could be found for all components except the antibiotic. The validation method showed acceptable linearity of r2≥0.98. Accuracy and precision parameters showed RSD (%)<2. All relative error value estimates were below 4%. The proposed validation design for 32×32 cm antibiotic plates yielded valid results and can be projected for the routine Quality Control analysis of the antibiotic material, especially which is incorporated into a finished medicinal dosage form. Doi: 10.28991/HIJ-2021-02-04-04 Full Text: PDF

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Section: Validity Of the Linearity Curvementioning

confidence: 99%

Microbiological Antibiotic Assay Validation of Gentamicin Sulfate Using Two-Dose Parallel Line Model (PLM)

Eissa

Rashed²,

Eissa³

2021

HighTech. Innov. J.

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show abstract

“…The techniques for developing a model to describe this standard curve have been a topic of considerable study by several researchers (Voigt et al 1979). The use of a quadratic or higher order equation has been studied by Kavanagh (1977), and based on several vitamin assays has been found to be more accurate than a linear type model. The objective of this work is not to fit a specific model but rather to present two techniques to accurately draw and interpolate the standard curve: (1) by Lagrange's interpolating polynomials and (2) by a fourth degree polynomial regression.…”

Section: Introductionmentioning

confidence: 99%

Computer Codes and Their Application a Microcomputer Based Algorithm for Quantitative Determination of Vitamins From Turbidimetric Methods

Rao

Webb

Eitenmiller

1987

J Food Processing Preservation

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Turbidimetric determination of blood aminoglycoside levels by growth curve analysis

Edberg

1980

Journal of Pharmaceutical Sciences

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Microbiological Turbidimetric Methods: Linearization of Antibiotic and Vitamin Standard Curves

Cited by 5 publications

References 11 publications

Microbiological Antibiotic Assay Validation of Gentamicin Sulfate Using Two-Dose Parallel Line Model (PLM)

Microbiological Antibiotic Assay Validation of Gentamicin Sulfate Using Two-Dose Parallel Line Model (PLM)

Computer Codes and Their Application a Microcomputer Based Algorithm for Quantitative Determination of Vitamins From Turbidimetric Methods

Turbidimetric determination of blood aminoglycoside levels by growth curve analysis

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