Microbiology in burns patients with blood stream infections: trends over time and during the course of hospitalization

Sousa, Dolores; Ceniceros, Alejandra; Galeiras, Rita; Pértega-Díaz, Sonia; Gutiérrez-Urbón, José-María; Rodríguez-Mayo, María; López-Suso, Eugenia; Fariña, Mónica Mourelo; Llinares, Pedro

doi:10.1080/23744235.2017.1397738

Cited by 19 publications

(14 citation statements)

References 23 publications

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“…Pseudomonas aeruginosa is a major pathogen that often causes nosocomial infections in burn patients ( Revathi et al, 1998 ; Singh et al, 2003 ; Yali et al, 2014 ; Sousa et al, 2017 ). The rapid increase of multidrug resistance and even carbapenem resistance exhibited by this bacterium makes it a serious problem in burn centers ( Dou et al, 2017 ; Sousa et al, 2017 ). Our previous study showed that P. aeruginosa is consistently one of the top three microorganisms isolated in our center ( Yali et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…Pseudomonas aeruginosa is one of the most common pathogens isolated from burn patients throughout the world ( Revathi et al, 1998 ; Singh et al, 2003 ; Yali et al, 2014 ; Dou et al, 2017 ; Sousa et al, 2017 ). The increasing prevalence of multidrug-resistant (MDR), extensively drug resistant (XDR), and pandrug-resistant (PDR) P. aeruginosa poses a grim challenge for antimicrobial therapy ( El Zowalaty et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, carbapenems are the main antibiotics used for treating MDR P. aeruginosa infections. However, carbapenem resistance is increasing year by year especially in the isolates collected from burn patients ( Dou et al, 2017 ; Sousa et al, 2017 ) and thus presents a difficult challenge for clinicians. Carbapenem resistance in P. aeruginosa is usually multifactorial and can be caused by several different mechanisms ( Castanheira et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Typing and Carbapenem Resistance Mechanisms of Pseudomonas aeruginosa Isolated From a Chinese Burn Center From 2011 to 2016

et al. 2018

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Pseudomonas aeruginosa is the leading cause of infection in burn patients. The increasing carbapenem resistance of P. aeruginosa has become a serious challenge to clinicians. The present study investigated the molecular typing and carbapenem resistance mechanisms of 196 P. aeruginosa isolates from the bloodstream and wound surface of patients in our burn center over a period of 6 years. By multilocus sequence typing (MLST), a total of 58 sequence types (STs) were identified. An outbreak of ST111, a type that poses a high international risk, occurred in 2014. The isolates from wound samples of patients without bacteremia were more diverse and more susceptible to antibiotics than strains collected from the bloodstream or the wound surface of patients with bacteremia. Importantly, a large proportion of the patients with multisite infection (46.51%) were simultaneously infected by different STs in the bloodstream and wound surface. Antimicrobial susceptibility testing of these isolates revealed high levels of resistance to carbapenems, with 35.71% susceptibility to imipenem and 32.14% to meropenem. To evaluate mechanisms associated with carbapenem resistance, experiments were conducted to determine the prevalence of carbapenemase genes, detect alterations of the oprD porin gene, and measure expression of the ampC β-lactamase gene and the mexB multidrug efflux gene. The main mechanism associated with carbapenem resistance was mutational inactivation of oprD (88.65%), accompanied by overexpression of ampC (68.09%). In some cases, oprD was inactivated by insertion sequence element IS1411, which has not been found previously in P. aeruginosa. These findings may help control nosocomial P. aeruginosa infections and improve clinical practice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Typing and Carbapenem Resistance Mechanisms of Pseudomonas aeruginosa Isolated From a Chinese Burn Center From 2011 to 2016

et al. 2018

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“…who detected that P. aeruginosa bacteria were found to be the predominant bacteria in burns. Skin is considered as one of the main mechanical immune barriers that provide protection support against pathogens; however, any destruction facing this barrier may leave the body prone to these opportunistic microorganisms encouraging for nosocomial infections of burns (Russotto et al, 2015;Sousa et al, 2018). The results of the study showed that the most frequent isolated bacteria from burns were the carbapenem-resistant P. aeruginosa (66.67%) recovered from admitted burnt patients who were receiving treatment in the Burn Hospital, Al-Diwaniyah City.…”

Section: Discussionmentioning

confidence: 98%

Molecular Detection of Serine Carbapenemase Genes in Carbapenem-Resistant Isolates of Pseudomonas aeruginosa Recovered from Patients in Al-Diwaniyah Province, Iraq

Al-abedi¹,

Al-Mayahi²

2019

J Pure Appl Microbiol

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The present study aimed at investigating the spread of serine carbapenemase-producing isolates of Pseudomonas aeruginosa recovered from some hospitals and specialized health centers in Al-Diwaniyah City, Iraq. Using morphological and molecular methods, 630 clinical samples (244 burn swabs, 163 sputa, 115 urine samples, and 108 ear swabs) were tested. The current work also explored the carbapenemase production ability of the carbapenemresistant (CR) isolates using morphological and molecular methods. A monoplex polymerase chain reaction (MPCR) technique targeting 4 different serine carbapenemase (SCMs) coding genes was followed. The study also explored the nucleotide sequences of the SCM genes. The API 20 E and Vitek 2 system showed that 100 of the isolates belonged to P. aeruginosa. With different severity of resistance by percentages of isolates to various antibiotics, only 24% of the isolates displayed resistance against carbapenem. The results of the SCM-related MPCR showed the occurrence of the bla GES , bla sMe , bla KPC , and bla OXA-48 genes in 20 (83.3%), 19 (79.1%), 11 (45.8%), 18 (75%), respectively, of the isolates. The phylogenetic study analysis revealed high, 99%, identity with NCBI-related world strains. According to the best of our knowledge, this is the first study in Iraq that detects a group of encoding genes of serine carbapenemase in carbapenem-resistant isolates.

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“…Pseudomonas aeruginosa is an opportunistic human pathogen responsible for nosocomial infections worldwide, such as ventilator-associated pneumonia and burn wound infections, mainly in immunosuppressed patients (De Bentzmann and Plesiat, 2011;Gellatly and Hancock, 2013;Cabot et al, 2016;Sousa et al, 2018;Yin et al, 2018). Moreover, it is highly involved in chronic pneumonia in patients with cystic fibrosis (CF) (Lyczak et al, 2000;Breidenstein et al, 2011;Ballarini et al, 2012;Lopez-Causape et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

A Core Genome Multilocus Sequence Typing Scheme for Pseudomonas aeruginosa

et al. 2020

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Pseudomonas aeruginosa is a ubiquitous microorganism and an important opportunistic pathogen responsible for a broad spectrum of infections mainly in immunosuppressed and critically ill patients. Molecular investigations traditionally rely on pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). In this work we propose a core genome multilocus sequence typing (cgMLST) scheme for P. aeruginosa, a methodology that combines traditional MLST principles with whole genome sequencing data. All publicly available complete P. aeruginosa genomes, representing the diversity of this species, were used to establish a cgMLST scheme targeting 2,653 genes. The scheme was then tested using genomes available at contig, chromosome and scaffold levels. The proposed cgMLST scheme for P. aeruginosa typed over 99% (2,314/2,325) of the genomes available for this study considering at least 95% of the cgMLST target genes present. The absence of a certain number gene targets at the threshold considered for both the creation and validation steps due to low genome sequence quality is possibly the main reason for this result. The cgMLST scheme was compared with previously published whole genome single nucleotide polymorphism analysis for the characterization of the population structure of the epidemic clone ST235 and results were highly similar. In order to evaluate the typing resolution of the proposed scheme, collections of isolates belonging to two important STs associated with cystic fibrosis, ST146 and ST274, were typed using this scheme, and ST235 isolates associated with an outbreak were evaluated. Besides confirming the relatedness of all the isolates, earlier determined by MLST, the higher resolution of cgMLST denotes that it may be suitable for surveillance programs, overcoming possible shortcomings of classical MLST.

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Microbiology in burns patients with blood stream infections: trends over time and during the course of hospitalization

Cited by 19 publications

References 23 publications

Molecular Typing and Carbapenem Resistance Mechanisms of Pseudomonas aeruginosa Isolated From a Chinese Burn Center From 2011 to 2016

Molecular Typing and Carbapenem Resistance Mechanisms of Pseudomonas aeruginosa Isolated From a Chinese Burn Center From 2011 to 2016

Molecular Detection of Serine Carbapenemase Genes in Carbapenem-Resistant Isolates of Pseudomonas aeruginosa Recovered from Patients in Al-Diwaniyah Province, Iraq

A Core Genome Multilocus Sequence Typing Scheme for Pseudomonas aeruginosa

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