2022
DOI: 10.1139/cjm-2021-0225
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Microbiology of barrier component analogues of a deep geological repository

Abstract: Canada is currently implementing a site selection process to identify a location for a deep geological repository (DGR) for the long-term storage of Canada’s used nuclear fuel, wherein used nuclear fuel bundles will be sealed inside copper-coated carbon steel containers, encased in highly compacted bentonite clay buffer boxes and sealed deep underground in a stable geosphere. Because a DGR must remain functional for a million years, there is value to examining ancient natural systems that serve as analogues fo… Show more

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Cited by 6 publications
(5 citation statements)
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References 160 publications
(260 reference statements)
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“…6 ). The absence of ASVs detected in direct DNA extracts from cultivation efforts for bentonite and subsurface rock samples has been reported previously ( 7 , 8 ). Such discrepancies are expected and may be due to DNA extraction bias (e.g., DNA absorption to clay, incomplete lysis, relic DNA), amplification (e.g., primers, amplification conditions), sequencing (e.g., insufficient coverage), but most importantly culture conditions (e.g., unknown nutritional requirements).…”
Section: Discussionsupporting
confidence: 80%
See 1 more Smart Citation
“…6 ). The absence of ASVs detected in direct DNA extracts from cultivation efforts for bentonite and subsurface rock samples has been reported previously ( 7 , 8 ). Such discrepancies are expected and may be due to DNA extraction bias (e.g., DNA absorption to clay, incomplete lysis, relic DNA), amplification (e.g., primers, amplification conditions), sequencing (e.g., insufficient coverage), but most importantly culture conditions (e.g., unknown nutritional requirements).…”
Section: Discussionsupporting
confidence: 80%
“…In particular, highly compacted bentonite can inhibit microbial growth when exceeding 1.6 g/cm 3 dry density, which corresponds to water activity below 0.96 and swelling pressures exceeding 2 MPa ( 9 ), and it was shown that swelling bentonites decrease sulfide production by SRB ( 10 ). A study featuring ancient analog samples reported low cultivable heterotroph and SRB associated with a bentonite deposit that was formed ~10 million years ago and sequencing of SRB cultures revealed that the taxon present was affiliated with the genus Desulfosporosinus ( 7 ). An analysis of dry, uncompacted bentonite clay samples showed that cultivation-based approaches were consistently associated with phylogenetically similar SRB, aerobic heterotrophs, and fermenters ( 8 ).…”
Section: Introductionmentioning
confidence: 99%
“…Despite the harsh living conditions in OPA rock, we identified a diverse microbial community (Figures A2 , A3 , A5 , and A7 ) with Gammaproteobacteria dominating over Alphaproteobacteria, followed by the phylum Firmicutes (Figures 4 and A1 ). The dominant phyla inherent to OPA were also identified by DNA‐based approaches in other natural clay formations such as bentonite (Beaver et al, 2022 ; Lopez‐Fernandez et al, 2015 ) and Boom Clay (Boivin‐Jahns et al, 1996 ), or by culture‐dependent approaches in bentonite (López‐Fernández et al, 2014 ).…”
Section: Discussionmentioning
confidence: 99%
“…The most similar microbial communities were obtained from samples extracted in the same laboratory (Bagnoud et al, 2015; Moll et al, 2013), suggesting contamination during DNA extraction (Bagnoud et al, 2015). The most recent study generated inconsistent replicate 16S ribosomal RNA (rRNA) gene profiles that were associated primarily with contaminant sequences, again suggesting that the microbial profiles detected were not sample‐specific (Beaver et al, 2022).…”
Section: Introductionmentioning
confidence: 99%
“…Puc-57kan was chosen due to the ability of kanamycin to function under low pH conditions. (Krause et al, 2017Beaver et al, 2022.…”
Section: Introductionmentioning
confidence: 99%