Microbiology of periodontitis in diabetic patients in Oran, Algeria

Abstract

Background: Poorly controlled diabetes increases the risk of periodontitis. The link between diabetes and periodontal disease is increasingly recognized and that diabetics are more susceptible to periodontal disease. Material and methods: Cultures of sub-gingival plaque from 62 subjects suffering from aggressive periodontitis (37 patients with diabetes and 25 control cases) and 136 patients with chronic periodontitis (92 with diabetes and 44 control cases) were done on selective and nonselective media. The bacterial isolates were identified by conventional biochemical tests. Results: In aggressive periodontitis, Actinomyces israelii was present in patients with diabetes only while Actinomyces naeslundii was positive in the control group only. The rates of Eubacterium nodatum, Capnocytophaga spp., Peptostreptococcus anaerobius, and Eikenella corrodens. Actinobacillus actinomycetemcomitans were remarkably present in diabetic patients. Culture of Prevotella intermedia, Eubacterium spp and Campylobacter spp were more remarkable in control patients with aggressive periodontitis. On the other hand, in chronic periodontitis, Eikenella corrodens, Aggregatibacter aphrophilus, and Eubacterium were more noticeable in patients with diabetes. Conclusions: Periodontal pathogens are distinctly different in patients with diabetes from those without diabetes. The differences are evident in both aggressive and chronic periodontitis. These different microbiological characteristics are relevant in both dental and general medical practices.

In vitro study on the activity of essential oil and methanolic extract from Algerian Nigella sativa L. Seeds on the growth kinetics of micro-organisms isolated from the buccal cavities of periodontal patients

“…After rinsing the mouth with sterile distilled water, supragingival plaques were removed by sterile swabs moistened with physiological saline solution, while subgingival plaques were collected on a Gracey curette inserted into the periodontal pocket. The samples were placed in sterile tubes and immediately transported to the laboratory (Yacoubi et al, 2010, Colombier et al, 2006).…”

Relative Reduction of the Biological and phylogenetic diversity of oral microbiome in diabetic and pre-diabetic subjects

“…In our study we selected subjects and diabetic patients that are either free of any periodontitis or having mild periodontitis to diminish the effect of oral health on the oral microbiome structure and attempt to make any observed changes in the oral flora is due to the glycemic status only as much as possible. Clear changes in the oral flora were found in diabetic patients with both aggressive or chronic periodontitis [11] [27] [28]. In this study, there is a clear reduction in the number of species (OTUs) observed in both impaired glucose tolerance and in the diabetic group when compared with the normoglycemic subjects.…”

Levofloxacin HCl-Loaded Eudragit L-Based Solvent Exchange-Induced In Situ Forming Gel Using Monopropylene Glycol as a Solvent for Periodontitis Treatment

“…In spite of this, more research needs to be carried out to determine the specific roles that each of these species plays, either on its own or in conjunction with other species, in the pathogenesis of periodontal breakdown. Staphylococcus aureus could be occasionally isolated from the periodontal pockets of patients with aggressive periodontitis, and Escherichia coli is sometimes declared as a microorganism in patients with periodontitis [88]. In contrast to the vast majority of other types of infections, all of the organisms that are thought to cause periodontal disease are native to the oral flora, Candida albicans courses of refractory periodontitis [89].…”