Microbiology Test Reliability in Differentiation of Neisseria meningitidis and Neisseria polysaccharea

Deák, Eszter; Green, N.; Humphries, Romney M.

doi:10.1128/jcm.01703-14

Cited by 6 publications

(6 citation statements)

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“…The organism is oxidase positive and oxidizes glucose and maltose, though other nonpathogenic Neisseria spp. can produce the same reactions (2). Identification of N. meningitidis is FDA cleared on the two current commercially available MALDI-TOF MS systems in the United States, as well as several of the manual and automated biochemical systems; however, no system is 100% specific, and results should be interpreted with caution.…”

Section: Discussionmentioning

confidence: 99%

“…Similar misidentifications or inability to identify pathogenic Neisseria spp. have been reported for some rapid biochemical assays (2). For definitive identification, a mix of rapid acid detection, enzyme production, and colistin resistance tests, as well as observation of pigment, may be needed (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…For definitive identification, a mix of rapid acid detection, enzyme production, and colistin resistance tests, as well as observation of pigment, may be needed (Table 1). Ultimately, 16S ribosomal sequencing may be necessary for some select cases with unusual phenotypic test results (2). Results should always be The alternative pathway is also activated by direct interaction between the bacterial surface and C3.…”

Section: Discussionmentioning

confidence: 99%

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The Brief Case: Meningococcemia Leading to a Diagnosis of Complement Deficiency in a 23-Month-Old

Brostowski

Graf

2019

J Clin Microbiol

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Brief Case: Meningococcemia Leading to a Diagnosis of Complement Deficiency in a 23-Month-Old

Brostowski

Graf

2019

J Clin Microbiol

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“…However, as N. cinerea is one of the asaccharolytic species, specific identification by metabolic testing is difficult. Neisseria meningitidis can virtually be excluded with phenotypic tests, although misidentification of N. meningitidis is possible, as described recently (24).…”

Section: Case Reportmentioning

confidence: 98%

Meningitis and Bacteremia Due to Neisseria cinerea following a Percutaneous Rhizotomy of the Trigeminal Ganglion

et al. 2016

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e Neisseria cinerea is a human commensal. The first known case of meningitis and bacteremia due to Neisseria cinerea following percutaneous glycerol instillation of the trigeminal ganglion is reported. Conventional phenotypic methods and complete 16S RNA gene sequencing accurately identified the pathogen. Difficulties in differentiation from pathogenic neisseriae are discussed. CASE REPORTA 58-year-old male patient presented the day after a percutaneous glycerol rhizotomy of the trigeminal ganglion with fever (38.2°C), clinical signs of meningitis without new focal neurologic deficits, and a normal neurocognitive status.His personal history was remarkable for severe trigeminal neuralgia refractory to medical treatment and for diagnosis of primary progressive multiple sclerosis for 13 years without immunosuppressive treatment.His white blood cell count (18.9 G/liter; 90% neutrophils, no left shift) and his C-reactive protein level (189 mg/dl) were elevated. Two sets of blood cultures and a lumbar puncture were immediately performed, and empirical antibiotic treatment with intravenous (i.v.) ceftriaxone administered in two doses at 2 g per dose (2x2g) was started. The cerebrospinal fluid (CSF) sample showed 4,125 nuclear cells/l, with 89% polymorphonuclear neutrophils. Protein and glucose (GLU) levels were abnormal, with 1.32 g (normal, 0.15 to 0.45) of protein/liter and 0.29 mmol (normal, 2.2 to 3.9) of glucose/liter in the CSF. Gram staining of the CSF revealed Gram-labile cocci.A computed tomography (CT) scan showed a small amount of intracerebral air near the trigeminal ganglion, confirming accidental involvement of intracerebral structures during rhizotomy (Fig. 1A). Flucloxacillin (6x2g) was added to the regimen. Droplet isolation precautions were not considered necessary because the clinical picture was suggestive for iatrogenic complication and not for meningococcal meningitis (1, 2).The following day, Neisseria species, susceptible to penicillin, ampicillin, ceftriaxone, and ciprofloxacin, grew in one of two sets of blood cultures and the CSF cultures (Table 1). Flucloxacillin treatment was stopped, and ceftriaxone treatment was continued for 7 days in total, according to the recommended treatment duration for meningitis due to N. meningitidis (3). The patient recovered rapidly. After 1 week, he was asymptomatic, with normal blood values.The CSF isolate was identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis (MALDI BioTyper; Bruker Daltonik, Bremen, Germany) as Neisseria meningitidis, with a score of 1.82 (sufficient only for genus assignment). Neisseria cinerea was the third species proposed by MALDI-TOF MS, with a minimal difference in the score (1.81). The colony morphology turned out to show no pigmentation. API NH V3.0 (bioMérieux Suisse SA, Geneva, Switzerland) testing was performed, with a positive reaction only for ProA, consistent with N. cinerea, and negative test results for glucose (GLU), maltose (MAL), and ␥-glutamyl aminopeptidas...

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“…Based on an evaluation on 200 samples carried out by Deak et al, this POC demonstrated a selectivity of 100% and sensitivity of 96% with a total assay time of 73 min. [173] One of the highlighted benefits of AmpliVue was its ability to process 24 different samples at the same time. In a 2020 publication, a highly promising disposable and extremely cheap (0.35 USD) silicon-based all-in-one micro-qPCR POC (Point-of-Need transducer (TriSilix)) was developed by Nunez-Bajo and coworkers for the real-time detection of the bacterial species, Mycobacterium avium subspecies paratuberculosis (M. a. subsp.…”

Section: Introduction To Commercially Available Poc Devicesmentioning

confidence: 99%

Biosensors and Point‐of‐Care Devices for Bacterial Detection: Rapid Diagnostics Informing Antibiotic Therapy

Gopal

Kashif

et al. 2021

Adv Healthcare Materials

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With an exponential rise in antimicrobial resistance and stagnant antibiotic development pipeline, there is, more than ever, a crucial need to optimize current infection therapy approaches. One of the most important stages in this process requires rapid and effective identification of pathogenic bacteria responsible for diseases. Current gold standard techniques of bacterial detection include culture methods, polymerase chain reactions, and immunoassays. However, their use is fraught with downsides with high turnaround time and low accuracy being the most prominent. This imposes great limitations on their eventual application as point-of-care devices. Over time, innovative detection techniques have been proposed and developed to curb these drawbacks. In this review, a systematic summary of a range of biosensing platforms is provided with a strong focus on technologies conferring high detection sensitivity and specificity. A thorough analysis is performed and the benefits and drawbacks of each type of biosensor are highlighted, the factors influencing their potential as point-of-care devices are discussed, and the authors' insights for their translation from proof-of-concept systems into commercial medical devices are provided.

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Microbiology Test Reliability in Differentiation of Neisseria meningitidis and Neisseria polysaccharea

Cited by 6 publications

References 4 publications

The Brief Case: Meningococcemia Leading to a Diagnosis of Complement Deficiency in a 23-Month-Old

The Brief Case: Meningococcemia Leading to a Diagnosis of Complement Deficiency in a 23-Month-Old

Meningitis and Bacteremia Due to Neisseria cinerea following a Percutaneous Rhizotomy of the Trigeminal Ganglion

Biosensors and Point‐of‐Care Devices for Bacterial Detection: Rapid Diagnostics Informing Antibiotic Therapy

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