Microbiome of the Successful Freshwater Invader, the Signal Crayfish, and Its Changes along the Invasion Range

Dragičević, Paula; Bielen, Ana; Petrić, Ines; Vuk, Marija; Žučko, Jurica; Hudina, Sandra

doi:10.1128/spectrum.00389-21

Cited by 17 publications

(15 citation statements)

References 91 publications

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“…In this study, potential bacterial causative agents were examined by comparing the bacterial composition of hepatopancreas between the individuals exhibiting mild and moderate histopathology. Multiple studies have recorded bacteria in hepatopancreas of crustaceans (Cheung et al, 2015;Dragičević et al, 2021b;Wu et al, 2021;Zhang et al, 2020). Additionally, some studies (Dragičević et al, 2021b;Zhang et al, 2020) found similarities between bacterial communities of hepatopancreas and other tissues, suggesting that bacteria are shared between tissues and that tissues of healthy crustaceans are not sterile.…”

Section: Discussionmentioning

confidence: 99%

“…Multiple studies have recorded bacteria in hepatopancreas of crustaceans (Cheung et al, 2015;Dragičević et al, 2021b;Wu et al, 2021;Zhang et al, 2020). Additionally, some studies (Dragičević et al, 2021b;Zhang et al, 2020) found similarities between bacterial communities of hepatopancreas and other tissues, suggesting that bacteria are shared between tissues and that tissues of healthy crustaceans are not sterile. Due to high abundance of bacteria from order Rickettsiales and class Mollicutes which also includes highly abundant genus Candidatus Hepatoplasma, their role in health and immunity of crustaceans needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

“…Sites S1 and S4 represent upstream and downstream invasion fronts, while sites S2 and S3 upstream and downstream invasion cores. Sites S4 and S5 are sampling locations of the native narrow-clawed crayfish Pontastacus leptodactylus (white circles) signal crayfish (Dragičević et al, 2021b), we discovered previously unrecorded gross morphological changes on hepatopancreas of several individuals. Hence, we decided to further investigate this finding and used available left lobe of hepatopancreas preserved in fixative for histological examination.…”

Section: Calculation Of Organosomatic Condition Indices and Tissue Samplingmentioning

confidence: 90%

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Disturbance in invasion? Idiopathic necrotizing hepatopancreatitis in the signal crayfish Pacifastacus leniusculus (Dana, 1852) in Croatia

Bekavac

Beck

Dragičević

et al. 2021

Journal of Fish Diseases

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As the most successful crayfish invader and possible vector for infectious agents, signal crayfish Pacifastacus leniusculus is among the major drivers of the native crayfish species decline in Europe. We describe histopathological manifestation and frequency of newly detected idiopathic necrotizing hepatopancreatitis along the invasion range of the signal crayfish in the Korana River in Croatia. Our results show extremely high prevalence of necrotizing hepatopancreatitis (97.3%), with 58.9% of individuals displaying mild and 31.5% moderate histopathological changes in the hepatopancreas, also reflected in the lower hepatosomatic index of analysed animals. Recorded histopathological changes were more frequent in the invasion core where population density is higher. Our preliminary screening of co-occurring native narrow-clawed crayfish Pontastacus leptodactylus showed lower incidence (33.3%) and only mild hepatopancreatic lesions, but potentially highlighted the susceptibility of native crayfish populations to this disease. Pilot analyses of dissolved trace and macro elements in water, sediment fractions and crayfish hepatopancreas do not highlight alarming or unusually high concentrations of analysed elements. Hepatopancreas microbiome analysis, using 16S rRNA gene amplicon sequencing, identified taxonomic groups that should be further investigated, along with impacts of the disease on health and viability of both invasive and native crayfish populations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Calculation Of Organosomatic Condition Indices and Tissue Samplingmentioning

confidence: 90%

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Disturbance in invasion? Idiopathic necrotizing hepatopancreatitis in the signal crayfish Pacifastacus leniusculus (Dana, 1852) in Croatia

Bekavac

Beck

Dragičević

et al. 2021

Journal of Fish Diseases

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“…The observed significant differences in immune response between the upstream and downstream signal crayfish populations might be due to differences in microhabitat characteristics. The approximately 33 km-long invasion range of the signal crayfish in the Korana River consists of microhabitats which differ in temperature, surrounding vegetation, water depth, sediment type, and anthropogenic pressure, with the upstream part of the Korana River flowing through the sparsely populated rural region, and the downstream part of the invasion range located in the industrial zone of the Karlovac City [ 104 ]. Additionally, it has been previously reported that different environmental parameters (e.g., temperature, pollution, oxygen levels, pH, and salinity) may affect crayfish immune system/health status [ 93 , 105 , 106 , 107 , 108 , 109 , 110 ].…”

Section: Discussionmentioning

confidence: 99%

Immune Response in Crayfish Is Species-Specific and Exhibits Changes along Invasion Range of a Successful Invader

Dragičević

Grbin

Maguire

et al. 2021

Biology

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Immunity is an important component of invasion success since it enables invaders’ adaptation to conditions of the novel environment as they expand their range. Immune response of invaders may vary along the invasion range due to encountered parasites/microbial communities, conditions of the local environment, and ecological processes that arise during the range expansion. Here, we analyzed changes in the immune response along the invasion range of one of the most successful aquatic invaders, the signal crayfish, in the recently invaded Korana River, Croatia. We used several standard immune parameters (encapsulation response, hemocyte count, phenoloxidaze activity, and total prophenoloxidaze) to: i) compare immune response of the signal crayfish along its invasion range, and between species (comparison with co-occurring native narrow-clawed crayfish), and ii) analyze effects of specific predictors (water temperature, crayfish abundance, and body condition) on crayfish immune response changes. Immune response displayed species-specificity, differed significantly along the signal crayfish invasion range, and was mostly affected by water temperature and population abundance. Specific immune parameters showed density-dependent variation corresponding to increased investment in them during range expansion. Obtained results offer baseline insights for elucidating the role of immunocompetence in the invasion success of an invertebrate freshwater invader.

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“…Recent research has demonstrated that bacterial microbiomes can have pivotal roles in invasion ecology. In signal crayfish, the intestinal microbial communities may affect and be affected by range expansion [14]. A survey of Cambarus sciotensis showed that the microbial diversity and composition of carapace and gills are determined by interactions within the local environment and host microsites [15].…”

Section: Introductionmentioning

confidence: 99%

Differences in Intestinal Microbial Composition between Red Claw Crayfish (Cherax quadricarinatus) and Red Swamp Crayfish (Procambarus clarkii) Cultured in Pond

Chen

Liu

Ouyang

et al. 2022

Fishes

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Intestinal microbiota communities participate in several metabolic processes in the host, and are highly correlated to digestion, nutrition, growth, and immunity. However, the intestinal microbiota of aquatic invertebrates is poorly understood, especially in freshwater crayfish. In this study, the intestinal microbiota of two important freshwater economic aquaculture species, the invasive species, the red swamp crayfish (Procambarus clarkii, Pc), and the introduced species, the red claw crayfish (Cherax quadricarinatus, Cq), were investigated. The results showed that the community richness and diversity of Pc were higher than those of Cq, which might be one of the reasons that Pc have stronger environmental adaptability than Cq. Five core phyla were identified in the Pc group, including Proteobacteria (26.92%), Tenericutes (25.73%), Bacteroidetes (25.12%), Firmicutes (14.03%), and RsaHF231 (8.02%), and three phyla were detected in the Cq group, including Tenericutes (67.35%), Proteobacteria (25.98%), and Firmicutes (4.69%). In Pc and Cq groups, Proteobacteria exhibited significant differential abundance between males and females. In addition, Vibrio coralliilyticus were found particularly in the intestine of Cq. This study provides information on intestinal microbiota differences of Pc and Cq, contributing to the development of new dietary formulations and providing significance to future aquaculture.

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Microbiome of the Successful Freshwater Invader, the Signal Crayfish, and Its Changes along the Invasion Range

Cited by 17 publications

References 91 publications

Disturbance in invasion? Idiopathic necrotizing hepatopancreatitis in the signal crayfish Pacifastacus leniusculus (Dana, 1852) in Croatia

Disturbance in invasion? Idiopathic necrotizing hepatopancreatitis in the signal crayfish Pacifastacus leniusculus (Dana, 1852) in Croatia

Immune Response in Crayfish Is Species-Specific and Exhibits Changes along Invasion Range of a Successful Invader

Differences in Intestinal Microbial Composition between Red Claw Crayfish (Cherax quadricarinatus) and Red Swamp Crayfish (Procambarus clarkii) Cultured in Pond

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