Microbiome shifts and the inhibition of quorum sensing by Black Band Disease cyanobacteria

Meyer, Julie; Gunasekera, Sarath P.; Scott, Raymond M; Paul, Valerie J.; Teplitski, Max

doi:10.1038/ismej.2015.184

Cited by 92 publications

(104 citation statements)

References 79 publications

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“…This finding is consistent with other studies of coral disease-associated bacterial communities (e.g. Croquer et al 2013;Sweet et al 2013;Gignoux-Wolfsohn and Vollmer 2015;Meyer et al 2015).…”

Section: Community-level Effectssupporting

confidence: 93%

What is killing Caribbean corals? Investigating a devastating coral disease.

Gignoux-Wolfsohn¹

2014

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Increased bacterial diversity on diseased corals can obscure disease etiology and complicate our understanding of pathogenesis. To untangle microbes that may cause white band disease signs from microbes responding to disease, we inoculated healthy Acropora cervicornis corals with an infectious dose from visibly diseased corals. We sampled these dosed corals and healthy controls over time for sequencing of the bacterial 16S region. Endozoicomonas were associated with healthy fragments from 4/10 colonies, dominating microbiomes before dosing and decreasing over time only in corals that displayed disease signs, suggesting a role in disease resistance. We grouped disease-associated bacteria by when they increased in abundance (primary vs secondary) and whether they originated in the dose (colonizers) or the previously healthy corals (responders). We found that all primary responders increased in all dosed corals regardless of final disease state and are therefore unlikely to cause disease signs. In contrast, primary colonizers in the families Pasteurellaceae and Francisellaceae increased solely in dosed corals that ultimately displayed disease signs, and may be infectious foreign bacteria involved in the development of disease signs. Moving away from a static comparison of diseased and healthy bacterial communities, we provide a framework to identify key players in other coral diseases.

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Section: Community-level Effectssupporting

confidence: 93%

What is killing Caribbean corals? Investigating a devastating coral disease.

Gignoux-Wolfsohn¹

2014

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“…) and Roseofilum reptotaenium (Meyer et al. ). Interestingly, lyngbic acid also inhibits quorum sensing and may contribute to the pathogenicity of R. reptotaenium in black band disease on corals (Meyer et al.…”

Section: Discussionmentioning

confidence: 99%

Uncovering cryptic diversity of Lyngbya: the new tropical marine cyanobacterial genus Dapis (Oscillatoriales)

Engene

Tronholm

Paul

2018

Journal of Phycology

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Cyanobacteria comprise an extraordinarily diverse group of microorganisms and, as revealed by increasing molecular information, this biodiversity is even more extensive than previously estimated. In this sense, the cyanobacterial genus Lyngbya is a highly polyphyletic group composed of many unrelated taxa with morphological similarities. In this study, the new genus Dapis was erected from the genus Lyngbya, based on a combined molecular, chemical, and morphological approach. Herein, two new species of cyanobacteria are described: D. pleousa and D. pnigousa. Our analyses found these species to be widely distributed and abundant in tropical and subtropical marine habitats. Seasonally, both species have the ability to form extensive algal blooms in marine habitats: D. pleousa in shallow-water, soft bottom habitats and D. pnigousa on coral reefs below depths of 10 m. Electron microscopy showed that D. pleousa contains gas vesicles, a character not previously reported in Lyngbya. These gas vesicles, in conjunction with a mesh-like network of filaments that trap oxygen released from photosynthesis, provide this species with an unusual mechanism to disperse in coastal marine waters, allowing D. pleousa to be present in both benthic and planktonic forms. In addition, both D. pleousa and D. pnigousa contained nitrogen-fixing genes as well as bioactive secondary metabolites. Several specimens of D. pnigousa biosynthesized the secondary metabolite lyngbic acid, a molecule that has also been isolated from many other marine cyanobacteria. Dapis pleousa consistently produced the secondary metabolite malyngolide, which may provide a promising chemotaxonomic marker for this species.

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“…The abundance of biosynthetic clusters in the Roseofilum MAGs is consistent with the distribution of biosynthetic clusters in cyanobacterial genomes, as uncovered in a recent large-scale comparative genomics study (Shih et al, 2015). Natural products from marine cyanobacteria have been extensively explored for bioactive properties in the development of anti-inflammatory and anti-cancer drugs (Nunnery et al, 2010), though the intended purpose of these compounds from the perspective of cyanobacteria is potentially as grazing deterrents (Nagle and Paul, 1999), for UV protection (Sorrels et al, 2009) and allelopathy (Leão et al, 2012), or for interference in bacterial communication (quorum sensing inhibition; Dobretsov et al, 2010; Meyer et al, 2016). The Roseofilum MAGs contained the highest number of biosynthetic clusters, followed by Bacteroidetes MAGs, and the fewest clusters were detected in Proteobacteria MAGs.…”

Section: Resultsmentioning

confidence: 99%

Comparative Metagenomics of the Polymicrobial Black Band Disease of Corals

et al. 2017

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Black Band Disease (BBD), the destructive microbial consortium dominated by the cyanobacterium Roseofilum reptotaenium, affects corals worldwide. While the taxonomic composition of BBD consortia has been well-characterized, substantially less is known about its functional repertoire. We sequenced the metagenomes of Caribbean and Pacific black band mats and cultured Roseofilum and obtained five metagenome-assembled genomes (MAGs) of Roseofilum, nine of Proteobacteria, and 12 of Bacteroidetes. Genomic content analysis suggests that Roseofilum is a source of organic carbon and nitrogen, as well as natural products that may influence interactions between microbes. Proteobacteria and Bacteroidetes members of the disease consortium are suited to the degradation of amino acids, proteins, and carbohydrates. The accumulation of sulfide underneath the black band mat, in part due to a lack of sulfur oxidizers, contributes to the lethality of the disease. The presence of sulfide:quinone oxidoreductase genes in all five Roseofilum MAGs and in the MAGs of several heterotrophs demonstrates that resistance to sulfide is an important characteristic for members of the BBD consortium.

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Microbiome shifts and the inhibition of quorum sensing by Black Band Disease cyanobacteria

Cited by 92 publications

References 79 publications

What is killing Caribbean corals? Investigating a devastating coral disease.

What is killing Caribbean corals? Investigating a devastating coral disease.

Uncovering cryptic diversity of Lyngbya: the new tropical marine cyanobacterial genus Dapis (Oscillatoriales)

Comparative Metagenomics of the Polymicrobial Black Band Disease of Corals

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