Microbiome Special Issue: Food, Drugs, Diagnostics, and Built Environments

ÖzdemirVural,

doi:10.1089/omi.2018.0001

Cited by 2 publications

(3 citation statements)

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“…These results show that feeding practices affect the bacterial diversity and composition of the gut microbiome, which is associated with oxidative stress in the very low birth weight (VLBW) of preterm infants [8,45]. As reported by Marseglia et al [21], the 2018 Odzemir study shows how the serum neutrophil-lymphocyte ratio (NLR) appears to have a predictive role in the development of OS-related diseases, as in the case of BPD; the periods of hypoxia and reoxygenation are sensitive, use the activation of neutrophils, and are responsible for the increase in oxidative metabolism, thus forming species reactive to oxygen and nitrogen [ROS and reactive nitrogen species (RNS), respectively] [46]. If these toxic products are not inactivated, their high chemical reactivity leads to damage to a variety of cellular macromolecules, including proteins, carbohydrates, lipids, and nucleic acid; this damage can progress to the death of lung cells [21].…”

Section: Discussionmentioning

confidence: 99%

“…These are non-enzymatic products from the oxidation of amino acid-free radicals and other PUFAs, and are chemically stable. These compounds have been detected electively in lipid-rich tissues, such as brain tissue, which, being rich in polyunsaturated lipids, is highly sensitive to oxidative stress [ 16 , 17 , 18 , 19 , 20 , 21 , 25 , 29 , 30 , 33 , 34 , 35 , 40 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 61 , 65 , 66 , 67 ].…”

Section: Discussionmentioning

confidence: 99%

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Oxidative Stress in Preterm Infants: Overview of Current Evidence and Future Prospects

et al. 2020

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Preterm birth (PTB), defined as parturition prior to 37 weeks of gestation, is the leading cause of morbidity and mortality in the neonatal population. The incidence and severity of complications of prematurity increase with decreasing gestational age and birthweight. The aim of this review study is to select the most current evidence on the role of oxidative stress in the onset of preterm complication prevention strategies and treatment options with pre-clinical and clinical trials. We also provide a literature review of primary and secondary studies on the role of oxidative stress in preterm infants and its eventual treatment in prematurity diseases. We conducted a systematic literature search of the Medline (Pubmed), Scholar, and ClinicalTrials.gov databases, retroactively, over a 7-year period. From an initial 777 articles identified, 25 articles were identified that met the inclusion and exclusion criteria. Of these, there were 11 literature reviews: one prospective cohort study, one experimental study, three case-control studies, three pre-clinical trials, and six clinical trials. Several biomarkers were identified as particularly promising, such as the products of the peroxidation of polyunsaturated fatty acids, those of the oxidation of phenylalanine, and the hydroxyl radicals that can attack the DNA chain. Among the most promising drugs, there are those for the prevention of neurological damage, such as melatonin, retinoid lactoferrin, and vitamin E. The microbiome also has an important role in oxidative stress. In conclusion, the most recent studies show that a strong relationship between oxidative stress and prematurity exists and that, unfortunately, there is still little therapeutic evidence reported in the literature.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Oxidative Stress in Preterm Infants: Overview of Current Evidence and Future Prospects

et al. 2020

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“…11,12 Proteomics can offer multiple advantages by the identification and characterization of novel proteins which are directly or indirectly involved in drug resistance that could be used as potential targets in future. [13][14][15] In the last two decades, mass spectrometry (MS)based proteomics has been effectively used to study proteome of microorganism, protein-protein interactions, differentially expressed proteins in order to better understand the molecular mechanism of microbial pathogenesis, 16 and antibiotic resistance mechanisms. 17 Compared to other important gram-negative bacteria, there are limited proteomic studies on S. marcescens.…”

Section: Sample Preparation and Fractionationmentioning

confidence: 99%

Proteomic profiling of Serratia marcescens by high-resolution mass spectrometry

2020

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Introduction: Serratia marcescens, an opportunistic human pathogen, is reported as an important cause of nosocomial infection and outbreaks. Although the genome of S. marcescens (ATCC 13880) was completely sequenced by 2014, there are no studies on the proteomic profile of the organism. The objective of the present study is to analyze the protein profile of S. marcescens (ATCC 13880) using a high resolution mass spectrometry (MS). Methods: Serratia marcescens ATCC 13880 strain was grown in Luria-Bertani broth and the protein extracted was subjected to trypsin digestion, followed by basic reverse phase liquid chromatography fractionation. The peptide fractions were then analysed using Orbitrap Fusion Mass Spectrometry and the raw MS data were processed in Proteome Discoverer software. Results: The proteomic analysis identified 15 009 unique peptides mapping to 2541 unique protein groups, which corresponds to approximately 54% of the computationally predicted protein-coding genes. Bioinformatic analysis of these identified proteins showed their involvement in biological processes such as cell wall organization, chaperone-mediated protein folding and ATP binding. Pathway analysis revealed that some of these proteins are associated with bacterial chemotaxis and beta-lactam resistance pathway. Conclusion: To the best of our knowledge, this is the first high-throughput proteomics study of S. marcescens (ATCC 13880). These novel observations provide a crucial baseline molecular profile of the S. marcescens proteome which will prove to be helpful for the future research in understanding the host-pathogen interactions during infection, elucidating the mechanism of multidrug resistance, and developing novel diagnostic markers or vaccine for the disease.

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Microbiome Special Issue: Food, Drugs, Diagnostics, and Built Environments

Cited by 2 publications

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Oxidative Stress in Preterm Infants: Overview of Current Evidence and Future Prospects

Oxidative Stress in Preterm Infants: Overview of Current Evidence and Future Prospects

Proteomic profiling of Serratia marcescens by high-resolution mass spectrometry

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