Microbiomes in the Intestine of Developing Pigs: Implications for Nutrition and Health

Mu, Chunlong; Pi, Yu; Zhang, Chuanjian; Zhu, Weiyun

doi:10.1007/978-3-030-85686-1_9

Cited by 5 publications

(2 citation statements)

References 99 publications

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“…Chenodeoxycholic acid (CDCA), also known as 3α,7α-dihydroxy-5β-cholan-24-oic acid, is one of the major primary BAs in the intestine of humans and animals. In pigs, hyocholic acid (HCA), CDCA, and cholic acid (CA) are the main primary BAs in the intestine, accounting for about 84%, 11%, and 4%, respectively [ 7 ]. CDCA has been applied in the clinical treatment of cholesterol gallstones in humans [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Chenodeoxycholic Acid (CDCA) Promoted Intestinal Epithelial Cell Proliferation by Regulating Cell Cycle Progression and Mitochondrial Biogenesis in IPEC-J2 Cells

Yan-pin

Wei

et al. 2022

Antioxidants

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Chenodeoxycholic acid (CDCA), a primary bile acid (BA), has been demonstrated to play an important role as a signaling molecule in various physiological functions. However, the role of CDCA in regulating intestinal epithelial cell (IEC) function remains largely unknown. Herein, porcine intestinal epithelial cells (IPEC-J2) were used as an in vitro model to investigate the effects of CDCA on IEC proliferation and explore the underlying mechanisms. IPEC-J2 cells were treated with CDCA, and flow cytometry and transcriptome analysis were adopted to investigate the effects and potential molecular mechanisms of CDCA on the proliferation of IECs. Our results indicated that adding 50 μmol/L of CDCA in the media significantly increased the proliferation of IPEC-J2 cells. In addition, CDCA treatment also hindered cell apoptosis, increased the proportion of G0/G1 phase cells in the cell cycle progression, reduced intracellular ROS, and MDA levels, and increased mitochondrial membrane potential, antioxidation enzyme activity (T-AOC and CAT), and intracellular ATP level (p < 0.05). RNA-seq results showed that CDCA significantly upregulated the expression of genes related to cell cycle progression (Cyclin-dependent kinase 1 (CDK1), cyclin G2 (CCNG2), cell-cycle progression gene 1 (CCPG1), Bcl-2 interacting protein 5 (BNIP5), etc.) and downregulated the expression of genes related to mitochondrial biogenesis (ND1, ND2, COX3, ATP6, etc.). Further KEGG pathway enrichment analysis showed that CDCA significantly enriched the signaling pathways of DNA replication, cell cycle, and p53. Collectively, this study demonstrated that CDCA could promote IPEC-J2 proliferation by regulating cell cycle progression and mitochondrial function. These findings provide a new strategy for promoting the intestinal health of pigs by regulating intestinal BA metabolism.

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Section: Introductionmentioning

confidence: 99%

Chenodeoxycholic Acid (CDCA) Promoted Intestinal Epithelial Cell Proliferation by Regulating Cell Cycle Progression and Mitochondrial Biogenesis in IPEC-J2 Cells

Yan-pin

Wei

et al. 2022

Antioxidants

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“…It was also shown that colistin-resistant E. coli and the mcr gene family were transmitted through food [24]. Other reports demonstrated that gut microbiota regulated host nutrition, immunity, and health [25]. Therefore, whether colistin sulfate alters the gastrointestinal tract microbiome, and whether these modifications are associated with health, requires more study.…”

Section: Discussionmentioning

confidence: 99%

Detection of Colistin Sulfate on Piglet Gastrointestinal Tract Microbiome Alterations

Yuan

Tian

et al. 2022

Veterinary Sciences

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The gut microbiome exerts important functions on host health maintenance, whereas excessive antibiotic use may cause gut flora dysfunction resulting in serious disease and dysbiosis. Colistin is a broad-spectrum antibiotic with serious resistance phenomena. However, it is unclear whether colistin alters the gastrointestinal tract microbiome in piglets. In this study, 16s rDNA-based metagenome analyses were used to assess the effects of colistin on the modification of the piglet microbiome in the stomach, duodenum, jejunum, cecum, and feces. Both α- and β-diversity indices showed that colistin modified microbiome composition in these gastrointestinal areas. In addition, colistin influenced microbiome composition at the phylum and genus levels. At the species level, colistin upregulated Mycoplasma hyorhinis, Chlamydia trachomatis, Lactobacillus agilis, Weissella paramesenteroides, and Lactobacillus salivarius abundance, but downregulated Actinobacillus indolicus, Campylobacter fetus, Glaesserella parasuis, Moraxella pluranimalium, Veillonella caviae, Neisseria dentiae, and Prevotella disiens abundance in stomachs. Colistin-fed piglets showed an increased abundance of Lactobacillus mucosae, Megasphaera elsdenii DSM 20460, Fibrobacter intestinalis, and Unidentified rumen bacterium 12-7, but Megamonas funiformis, Uncultured Enterobacteriaceae bacterium, Actinobacillus porcinus, Uncultured Bacteroidales bacterium, and Uncultured Clostridiaceae bacterium abundance was decreased in the cecum. In feces, colistin promoted Mucispirillum schaedleri, Treponema berlinense, Veillonella magna, Veillonella caviae, and Actinobacillus porcinus abundance when compared with controls. Taken together, colistin modified the microbiome composition of gastrointestinal areas in piglets. This study provides new clinical rationalization strategies for colistin on the maintenance of animal gut balance and human public health.

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Nutrition and Metabolism: Foundations for Animal Growth, Development, Reproduction, and Health

2021

Advances in Experimental Medicine and Biology

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Microbiomes in the Intestine of Developing Pigs: Implications for Nutrition and Health

Cited by 5 publications

References 99 publications

Chenodeoxycholic Acid (CDCA) Promoted Intestinal Epithelial Cell Proliferation by Regulating Cell Cycle Progression and Mitochondrial Biogenesis in IPEC-J2 Cells

Chenodeoxycholic Acid (CDCA) Promoted Intestinal Epithelial Cell Proliferation by Regulating Cell Cycle Progression and Mitochondrial Biogenesis in IPEC-J2 Cells

Detection of Colistin Sulfate on Piglet Gastrointestinal Tract Microbiome Alterations

Nutrition and Metabolism: Foundations for Animal Growth, Development, Reproduction, and Health

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