Microbioreactors as Engineering Tools for Bioprocess Development

Prado, R. C.; Borges, Elcio

doi:10.1590/0104-6632.20180354s20170433

Cited by 16 publications

(9 citation statements)

References 115 publications

(198 reference statements)

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“…Shake flask cultivation, the traditional screening method, allows for parallelization, but limited automation and labor intensity severely restrict efficient application in context with HTP approaches. Cultivation in microtiter plates represents an attractive cost-efficient alternative to shaker flasks, as they allow for even higher numbers of parallel cultivations, and the degree of automation via pipetting robotics can be significantly higher 20 , 21 .…”

Section: Introductionmentioning

confidence: 99%

High-throughput microbioreactor provides a capable tool for early stage bioprocess development

Fink

Cserjan‐Puschmann

Reinisch

et al. 2021

Sci Rep

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Tremendous advancements in cell and protein engineering methodologies and bioinformatics have led to a vast increase in bacterial production clones and recombinant protein variants to be screened and evaluated. Consequently, an urgent need exists for efficient high-throughput (HTP) screening approaches to improve the efficiency in early process development as a basis to speed-up all subsequent steps in the course of process design and engineering. In this study, we selected the BioLector micro-bioreactor (µ-bioreactor) system as an HTP cultivation platform to screen E. coli expression clones producing representative protein candidates for biopharmaceutical applications. We evaluated the extent to which generated clones and condition screening results were transferable and comparable to results from fully controlled bioreactor systems operated in fed-batch mode at moderate or high cell densities. Direct comparison of 22 different production clones showed great transferability. We observed the same growth and expression characteristics, and identical clone rankings except one host-Fab-leader combination. This outcome demonstrates the explanatory power of HTP µ-bioreactor data and the suitability of this platform as a screening tool in upstream development of microbial systems. Fast, reliable, and transferable screening data significantly reduce experiments in fully controlled bioreactor systems and accelerate process development at lower cost.

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Section: Introductionmentioning

confidence: 99%

High-throughput microbioreactor provides a capable tool for early stage bioprocess development

Fink

Cserjan‐Puschmann

Reinisch

et al. 2021

Sci Rep

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“…Turbulence is in fact rarely modelled due to the low Reynolds numbers obtained for these type of flows [19] and so is gravity [21,22], thus significantly simplifying the modelling. Simulating droplets and capillarity implies being able to computationally represent free surfaces between the oil and the air phases.…”

Section: Numerical Modelmentioning

confidence: 99%

CFD modelling of the effect of capillary pressure on retention behaviour of water menisci at inter-particle contacts

2021

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This paper presents a Computational Fluid Dynamics (CFD) model on the effect of capillary pressure on the retention behaviour of a granular material. The model proposes an unprecedented CFD insight into the onset of liquid menisci at the inter-particles contact under varying hydraulic conditions. The present work models the material grains as smooth spherical particles that define a porous network filled by two interstitial fluids: air and silicon oil. The numerical model has been subsequently validated against experimental measurements of the degree of saturation at different capillary pressures taken by Dullien et al. [F.A. Dullien, C. Zarcone, I.F. MacDonald, A. Collins, R.D. Bochard. J. Colloid Interface Sci. 127, 2 (1989)] in a system of smooth glass beads flooded with silicon oil. Results from the numerical simulations confirm the good capability of the model to reproduce the experimental retention behaviour of the granular material. Finally, the present paper laid the basis for future CFD studies on the effect of various factors (e.g. hydraulic hysteresis, surface roughness and/or grain shape) on the capillary pressure acting at the interparticle contact.

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“…Development of a miniaturized scale framework known as biochips, Bio-MEMS, or LOC [17][18][19], improves the applications for high-throughput biological screening [20], cell analysis, and clinical diagnostics [21], as well as point-of-care (POC) analysis for biomedical and environmental monitoring [22]. There are many unmatched advantages of microsystems over conventional analytical, chemical, and biomedical tools.…”

Section: Recent Development Of Integrated Microfluidics Biosensorsmentioning

confidence: 99%

Magnetic nanoparticles in microfluidic and sensing: From transport to detection

et al. 2020

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Superparamagnetic nanoparticles are attracting significant attention. Therefore, being explored in microsystems for a wide range of applications. Typical examples include labon-a-chip and microfluidics for synthesis, detection, separation, and transportation of different bioanalytes, such as biomolecules, cells, and viruses to develop portable, sensitive, and cost-effective biosensing systems. Particularly, microfluidic systems incorporated with magnetic nanoparticles and, in combination with magnetoresistive sensors, shift diagnostic and analytical methods to a microscale level. In this context, nanotechnology enables the miniaturization and integration of a variety of analytical functions in a single chip for manipulation, detection, and recognition of bioanalytes reliably and flexibly. In consideration of the above, recent development and benefits are elaborated herein to discuss the role of magnetic nanoparticles inside the microchannels to design highly efficient disposable point-of-care applications from transportation to the detection of bioanalytes.

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Microbioreactors as Engineering Tools for Bioprocess Development

Cited by 16 publications

References 115 publications

High-throughput microbioreactor provides a capable tool for early stage bioprocess development

High-throughput microbioreactor provides a capable tool for early stage bioprocess development

CFD modelling of the effect of capillary pressure on retention behaviour of water menisci at inter-particle contacts

Magnetic nanoparticles in microfluidic and sensing: From transport to detection

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