Microbiota and the plant immune system work together to defend against pathogens

Chakraborty, Joydeep

doi:10.1007/s00203-023-03684-9

Cited by 4 publications

(2 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Additionally, we found that the bacteria can induce the expression of genes related to jasmonic acid, salicylic acid, and ethylene ( Figure 7 ). Previous studies have reported the involvement of beneficial bacteria in the induction of the plant immune system, potentially facilitating the colonization processes of beneficial bacteria and enhancing defense against pathogens ( Tzipilevich et al., 2021 ; Chakraborty, 2023 ). When analyzing the genes expressed during tripartite interactions, we observed an increased expression of genes related to defense hormones, response to pathogens, and other genes associated with oxidative stress [UPOX and ZAT12], which could be involved in the induction of defense through ISR ( Davletova et al., 2005 ; Vaahtera et al., 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Amphibian skin bacteria display antifungal activity and induce plant defense mechanisms against Botrytis cinerea

Romero-Contreras,

Gonzalez-Serrano,

Formey

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Botrytis cinerea is the causal agent of gray mold, which affects a wide variety of plant species. Chemical agents have been used to prevent the disease caused by this pathogenic fungus. However, their toxicity and reduced efficacy have encouraged the development of new biological control alternatives. Recent studies have shown that bacteria isolated from amphibian skin display antifungal activity against plant pathogens. However, the mechanisms by which these bacteria act to reduce the effects of B. cinerea are still unclear. From a diverse collection of amphibian skin bacteria, three proved effective in inhibiting the development of B. cinerea under in vitro conditions. Additionally, the individual application of each bacterium on the model plant Arabidopsis thaliana, Solanum lycopersicum and post-harvest blueberries significantly reduced the disease caused by B. cinerea. To understand the effect of bacteria on the host plant, we analyzed the transcriptomic profile of A. thaliana in the presence of the bacterium C32I and the fungus B. cinerea, revealing transcriptional regulation of defense-related hormonal pathways. Our study shows that bacteria from the amphibian skin can counteract the activity of B. cinerea by regulating the plant transcriptional responses.

show abstract

Section: Discussionmentioning

confidence: 99%

Amphibian skin bacteria display antifungal activity and induce plant defense mechanisms against Botrytis cinerea

Romero-Contreras,

Gonzalez-Serrano,

Formey

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…O microbioma pode beneficiar as plantas por meio de diversos mecanismos, sendo os mais frequentemente descritos relacionados à mobilização e solubilização de nutrientes (Lazcano et al, 2021;Devi et al, 2022), à modulação da sinalização hormonal (Ravanbakhsh et al, 2018), à produção de antibióticos, incluindo metabólitos secundários e enzimas relacionadas a defesa (Helfrich et al, 2018), bem como à modulação do sistema imune vegetal (Teixeira et al, 2019;, Chakraborty, 2023.…”

Section: Funções Do Microbioma Vegetalunclassified

Avaliação funcional do microbioma da soja

Cunha

View full text Add to dashboard Cite

Functional evaluation of soybean microbiomeBrazil is the largest global producer and exporter of soybeans, the most consumed legume grain in the world. However, conventional farming methods rely heavily on chemical fertilizers and pesticides, leading to high costs and potential risks to the environment and human health. In this sense, the great diversity of microorganisms that live in association with plants (microbiomes) constitutes a promising alternative for the identification and development of new strategies to promote plant growth. Thus, this study evaluated a collection of bacteria associated with soybeans, the Soybiome, for functions that may influence plant development: (I) production of indole compounds, including auxin, (II) manipulation of ACC (1-aminocyclopropane-1-carboxylate; the precursor of ethylene) and (III) solubilization of inorganic nutrients (phosphate and calcite). In total, we evaluated 613 bacteria in multiple large-scale screenings, categorizing our collection into groups based on the different detected activities. Notably, a large majority of bacteria (80%) produced indole compounds, either constitutively or caused by tryptophan, the precursor of these metabolites. However, High Performance Liquid Chromatography (HPLC) analyzes revealed that a fraction of these compounds that correspond to the auxin indole-3-acetic acid (IAA) is variable and that a high production of indole compounds does not necessarily correspond to a high production of auxin. On the other hand, only 48 (7.8%) bacteria were able to grow in culture medium containing ACC as a source of nitrogen, indicating that the ability to degrade this molecule is rare among the isolates in our collection. In the phosphate and calcite solubilization experiments, we found 71 (11.6%) and 60 (9.8%) isolates, respectively, with the capacity to solubilize these nutrients in their inorganic form. Among these, 14 (2.3%) were able to solubilize both nutrients. A highlight in our study was the Cellulomonas PR1447 strain, the only one that showed a positive result in all assays. The results of this work suggest that the Soybiome collection can be a valuable source of bacteria with the potential to promote plant growth, mainly due to the diversity of bacteria found with multiple functions. This characterization is the first step in the systematic evaluation of our collection and will provide the basis for future tests on the plant using monoassociations and synthetic communities. In this way, we hope to contribute to the development of sustainable alternatives to the use of chemical fertilizers and agrochemicals in soybean cultivation.

show abstract

Re-Envisioning the Plant Disease Triangle: Full Integration of the Host Microbiota and a Focal Pivot to Health Outcomes

Leveau

2024

Annual Review of Phytopathology

View full text Add to dashboard Cite

The disease triangle is a structurally simple but conceptually rich model that is used in plant pathology and other fields of study to explain infectious disease as an outcome of the three-way relationship between a host, a pathogen, and their environment. It also serves as a guide for finding solutions to treat, predict, and prevent such diseases. With the omics-driven, evidence-based realization that the abundance and activity of a pathogen are impacted by proximity to and interaction with a diverse multitude of other microorganisms colonizing the same host, the disease triangle evolved into a tetrahedron shape, which features an added fourth dimension representing the host-associated microbiota. Another variant of the disease triangle emerged from the recently formulated pathobiome paradigm, which deviates from the classical “one pathogen” etiology of infectious disease in favor of a scenario in which disease represents a conditional outcome of complex interactions between and among a host, its microbiota (including microbes with pathogenic potential), and the environment. The result is a version of the original disease triangle where “pathogen” is substituted with “microbiota.” Here, as part of a careful and concise review of the origin, history, and usage of the disease triangle, I propose a next step in its evolution, which is to replace the word “disease” in the center of the host–microbiota–environment triad with the word “health.” This triangle highlights health as a desirable outcome (rather than disease as an unwanted state) and as an emergent property of host–microbiota–environment interactions. Applied to the discipline of plant pathology, the health triangle offers an expanded range of targets and approaches for the diagnosis, prediction, restoration, and maintenance of plant health outcomes. Its applications are not restricted to infectious diseases only, and its underlying framework is more inclusive of all microbial contributions to plant well-being, including those by mycorrhizal fungi and nitrogen-fixing bacteria, for which there never was a proper place in the plant disease triangle. The plant health triangle also may have an edge as an education and communication tool to convey and stress the importance of healthy plants and their associated microbiota to a broader public and stakeholdership.

show abstract

Microbiota and the plant immune system work together to defend against pathogens

Cited by 4 publications

References 83 publications

Amphibian skin bacteria display antifungal activity and induce plant defense mechanisms against Botrytis cinerea

Amphibian skin bacteria display antifungal activity and induce plant defense mechanisms against Botrytis cinerea

Avaliação funcional do microbioma da soja

Re-Envisioning the Plant Disease Triangle: Full Integration of the Host Microbiota and a Focal Pivot to Health Outcomes

Contact Info

Product

Resources

About