Microbiota Community Structure and Interaction Networks within Dermacentor silvarum, Ixodes persulcatus, and Haemaphysalis concinna

Qiu, Hongyu; Lv, Qingbo; Chang, Qiao‐Cheng; Ju, Hehua; Wu, Tingting; Liu, Shunshuai; Li, Xiuwen; Yan, Yimeng; Gao, Junfeng; Wang, Chun-Ren

doi:10.3390/ani12233237

Cited by 3 publications

(1 citation statement)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fifty female ticks with different satiated blood statuses were collected from the surface of tiger ( Figure 1 A,B) and deer ( Figure 1 C,D) at Changsha Ecological Zoo (28°1′ N, 113°0′ E), Hunan Province, China. The sample size referred to a recent similar study [ 16 ]. The tigers and deer lived in a stable environment within the same zoo, but two types of animals were housed in a relatively distant area (>0.5 km).…”

Section: Methodsmentioning

confidence: 99%

Composition of the Midgut Microbiota Structure of Haemaphysalis longicornis Tick Parasitizing Tiger and Deer

Liu,

Qiu,

Cheng

et al. 2024

Animals

View full text Add to dashboard Cite

Haemaphysalis longicornis is a common tick species that carries several pathogens. There are few reports on the influence of different hosts on the structure of midgut microflora in H. longicornis. In this study, midgut contents of fully engorged female H. longicornis were collected from the surface of tiger (Panthera tigris) and deer (Dama dama). The bacterial genomic DNA of each sample was extracted, and the V3–V4 regions of the bacterial 16S rRNA were sequenced using the Illumina NovaSeq sequencing. The diversity of the bacterial community of the fully engorged female H. longicornis on the surface of tiger was higher than that of deer. In total, 8 phyla and 73 genera of bacteria annotations were detected in the two groups. At the phylum level, the bacterial phyla common to the two groups were Proteobacteria, Firmicutes, and Actinobacteriota. At the genus level, there were 20 common bacterial genera, among which the relative abundances of Coxiella, Morganella, Diplorickettsia, and Acinetobacter were high. The Morganella species was further identified to be Morganella morganii. The alpha diversity index indicated that the bacterial diversity of the tiger group was higher than that of the deer group. Bacteroidota, Patescibacteria, Desulfobacterota, Verrucomicrobiota, and Cyanobacteria were solely detected in the tiger group. A total of 52 bacterial genera were unique in the tiger group, while one bacterial genus was unique in the deer group. This study indicates that there are differences in the structure of the gut bacteria of the same tick species among different hosts. Further culture-based methods are needed to provide a more comprehensive understanding of the tick microbiota parasitizing different hosts.

show abstract

Section: Methodsmentioning

confidence: 99%

Composition of the Midgut Microbiota Structure of Haemaphysalis longicornis Tick Parasitizing Tiger and Deer

Liu,

Qiu,

Cheng

et al. 2024

Animals

View full text Add to dashboard Cite

show abstract

Prevalence of Tick Infection with Bartonella in China: A Review and Meta-analysis

Wang,

Li,

Yin

et al. 2024

Acta Parasit.

View full text Add to dashboard Cite

Objective Bartonellosis is a global vector-borne zoonosis caused by Bartonella , a genus of intracellular Gram-negative bacteria. It is one of 14 emerging infectious diseases that have recently been identified in China, and the prevalence varies by region. A more in-depth understanding is needed regarding the role and influencing factors of ticks in the transmission of Bartonella , including the infection rate of ticks with Bartonella in different regions. This study explored the prevalence of Bartonella in ticks and the factors that influence it. Methods Databases (PubMed, Embase, Elsevier ScienceDirect, Cochrane Library, Web of Science, CNKI, VIP, CBM, and WanFang) were searched to review the preliminary research on Bartonella- carrying ticks in China. Results We identified and included 22 articles. Bartonella infection rates in ticks varied from 0 to 22.79% examined by the included studies. Our meta-analysis revealed that the prevalence of Bartonella in ticks was 3.15% (95% CI: 1.22 − 5.82%); the prevalence was higher in parasitic ticks (4.90%; 95% CI: 1.39 -10.14%) than ticks seeking hosts (1.42%; 95% CI: 0.62 − 2.50%) ( P = 0.047). Conclusion The prevalence of Bartonella in the southern region of China (6.45%) was higher than that in the northern region (1.28%) ( P = 0.030). Knowledge of ticks’ vectors and reservoir competence is crucial to reduce the disease burden. Supplementary Information The online version contains supplementary material available at 10.1007/s11686-024-00893-0.

show abstract

Microbiota profile in organs of the horseflies (Diptera: Tabanidae) in Northeastern China

Qiu,

Lv,

Wang

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Tabanids, commonly known as horseflies and belonging to the family Tabanidae, are blood-feeding arthropods (BFA) found worldwide. They are known for their ability to mechanically and biologically transmit various animal pathogens. Tabanids are potential vectors for diseases such as Francisella tularensis, Anaplasma marginale, Theileria spp., and contributors to lumpy skin diseases. Despite their involvement in common BFA studies, tabanids have not been extensively explored in microbiome research. In this study, the microbiota structure and composition in various organs of four distinct genera of tabanids: Atylotus, Haematopota, Tabanus, and Hybomitra were examined. High-throughput sequencing of the bacterial 16S rRNA gene was performed to gain insights into the microbial communities associated with the different tabanid species. Result display that microbiota composition and diversity, including Firmicutes, Proteobacteria, and Bacteroidetes, varied significantly among the different organs, with the ovaries exhibiting significantly higher diversity. Apart from the Haematopota genus, Tenericutes were enriched in the midgut of other tabanid species, whereas the Malpighian tubules exhibited a higher abundance of Bacteroides. Notably, the ovarian microbiota structure was conserved among the four tabanid species, indicating its potential association with reproductive development. Evaluation of the potential pathogen risk revealed putative pathogens in over 100 genera associated with these tabanid commensal organisms. Twenty genera were annotated as zoonotic agents with a high abundance of Citrobacter and Brucella, highlighting the presence of this important group of zoonotic pathogens. Functional predictions of vector-microbiota interactions indicate that microbiota significantly affects vector biological traits and can influence pathogen transmission via direct interactions or by regulating host immunity and nutrition. For the first time, the distribution characteristics and functions of four genera of horsefly microbiota were analyzed, revealing the presence of multiple potential pathogenic microorganisms. These findings provide valuable insights for future research and the development of symbiotic-based strategies to control insect-borne diseases among tabanids.

show abstract

Microbiota Community Structure and Interaction Networks within Dermacentor silvarum, Ixodes persulcatus, and Haemaphysalis concinna

Cited by 3 publications

References 53 publications

Composition of the Midgut Microbiota Structure of Haemaphysalis longicornis Tick Parasitizing Tiger and Deer

Composition of the Midgut Microbiota Structure of Haemaphysalis longicornis Tick Parasitizing Tiger and Deer

Prevalence of Tick Infection with Bartonella in China: A Review and Meta-analysis

Microbiota profile in organs of the horseflies (Diptera: Tabanidae) in Northeastern China

Contact Info

Product

Resources

About