Microbiota in Human Periodontal Abscess Revealed by 16S rDNA Sequencing

Abstract

Periodontal abscess is an oral infective disease caused by various kinds of bacteria. We aimed to characterize the microbiota composition of periodontal abscesses by metagenomic methods and compare it to that of the corresponding pocket and healthy gingival crevice to investigate the specific bacteria associated with this disease. Samples from abscess pus (AB), periodontal pocket coronally above the abscess (PO), and the gingival crevice of the periodontal healthy tooth were obtained from 20 periodontal abscess patients. Furthermore, healthy gingival crevice samples were obtained from 25 healthy individuals. Bacterial DNA was extracted and 16S rRNA gene fragments were sequenced to characterize the microbiota and determine taxonomic classification. The beta-diversity analysis results showed that the AB and PO groups had similar compositions. Porphyromonas gingivalis , Prevotella intermedia , and other Prevotella spp. were the predominant bacteria of human periodontal abscesses. The abundances of Filifactor alocis and Atopobium rimae were significantly higher in periodontal abscesses than in the periodontal pocket, suggesting their association with periodontal abscess formation. In conclusion, we characterized the microbiota in periodontal abscess and identified some species that are positively associated with this disease. This provides a better understanding of the components of periodontal abscesses, which will help facilitate the development of antibiotic therapy strategies.

Helicobacter Pylori Infection Increases the Incidence of Erosive Oral Lichen Planus and Alters the Oral Microbiome Composition

“…in the salivary microbiota of patients with OLP were signi cantly high, which suggested that these microbes are involved in the pathogenesis of OLP. Prevotella spp., a member of the oral microbiota, is abundant in patients with periodontal disease and periodontal abscesses [31]. Wang et al [21] and Du et al [32] reported that the relative abundance of Prevotella increased in the salivary microbiota of patients with OLP, which was consistent with the ndings of this study.…”

Alterations in subgingival microbiota during full‐fixed appliance orthodontic treatment—A prospective study

“…Selenomonas sputigena , Campylobacter gracilis and Leptotrichia have been associated with periodontitis previously 20,34,35 but the remaining species, namely Saccharibacteria (TM7), Prevotella denticola , Prevotella salivae , Atopobium rimae , Alloprevotella tannerae , Prevotella oulorum and Atopobium parvulum, were not associated with periodontitis. Saccharibacteria (TM7) are ubiquitous members of the human oral microbiome that have recently been identified through deep‐sequencing and appear to play a role in oral mucosal infectious diseases 36 ; Prevotella intermedia ( Pi ) is a commonly reported periodontopathogen (see below), but Prevotella denticola and Prevotella salivae were neither associated with orthodontic intervention nor periodontitis; Atopobium rimae may be associated with periodontal abscess formation 37 ; and Prevotella oulorum was associated with experimental gingivitis 38 . Further functional characterization of these additional bacteria, in particular Selenomonas sputigena , will improve our understanding of the impact of orthodontic intervention on oral health.…”

Arte y ciencia en el diagnóstico de la enfermedad periodontal atípica: reporte de caso

“…Mediante técnicas moleculares, se ha logrado aislar una flora bacteriana poco común, destacando las siguientes especies: Filifactor alocis, Enterococcus fecalis y de los géneros Desulfobulbus, Dialister. 14,15 Estudios recientes de metagenómica de la microbiota del absceso periodontal, susceptible de aparecer en una lesión endoperiodontal, subrayan la presencia de una flora no usual, como Filifactor y Atopobium rimae. 15 El efecto de la inflamación periodontal sobre la pulpa es un tema polémico y abundan las investigaciones con resultados contradictorios.…”

“…14,15 Estudios recientes de metagenómica de la microbiota del absceso periodontal, susceptible de aparecer en una lesión endoperiodontal, subrayan la presencia de una flora no usual, como Filifactor y Atopobium rimae. 15 El efecto de la inflamación periodontal sobre la pulpa es un tema polémico y abundan las investigaciones con resultados contradictorios. Se ha sugerido que la enfermedad periodontal no tiene consecuencia sobre la pulpa, al menos hasta que dañe al ápex.…”