Microbiota Induced Changes in the Immune Response in Pregnant Mice

Faas, Marijke M.; Liu, Yuanrui; Borghuis, Theo; Loo-Bouwman, Carolien A. Van; Harmsen, Hermie J. M.; Vos, Paul de

doi:10.3389/fimmu.2019.02976

Cited by 37 publications

(43 citation statements)

References 48 publications

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“…The expression of CD16 and CD14 allows the identification of three types of monocytes, i.e., classical, intermediate, and nonclassical. These cells are modified during pregnancy: the subpopulation of intermediate monocytes increases, while classical monocytes decrease and there are no changes in the nonclassical subpopulation 23 . Here, we show that all monocyte populations were not phenotypically different between infected or noninfected women, indicating that likely these cells do not participate in the creation of an inflammatory milieu.…”

Section: Resultsmentioning

confidence: 99%

Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection

et al. 2021

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SARS-CoV-2 infection can affect all human beings, including pregnant women. Thus, understanding the immunological changes induced by the virus during pregnancy is nowadays of pivotal importance. Here, using peripheral blood from 14 pregnant women with asymptomatic or mild SARS-CoV-2 infection, we investigate cell proliferation and cytokine production, measure plasma levels of 62 cytokines, and perform a 38-parameter mass cytometry analysis. Our results show an increase in low density neutrophils but no lymphopenia or gross alterations of white blood cells, which display normal levels of differentiation, activation or exhaustion markers and show well preserved functionality. Meanwhile, the plasma levels of anti-inflammatory cytokines such as interleukin (IL)-1RA, IL-10 and IL-19 are increased, those of IL-17, PD-L1 and D-dimer are decreased, but IL-6 and other inflammatory molecules remain unchanged. Our profiling of antiviral immune responses may thus help develop therapeutic strategies to avoid virus-induced damages during pregnancy.

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Section: Resultsmentioning

confidence: 99%

Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection

et al. 2021

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“…Then, we investigated whether the effects of supplemented feeds on NK cells and microbiota were related. Although similar analysis of correlation between immune cells and specific microbial taxa has been performed in mice [84], it has not been conducted in chickens. Both supplemented feeds enhanced CD107 expression on intraepithelial NK cells and increased relative abundance of commensal LAB at 7 days of age.…”

Section: Discussionmentioning

confidence: 99%

Glucose Oligosaccharide and Long-Chain Glucomannan Feed Additives Induce Enhanced Activation of Intraepithelial NK Cells and Relative Abundance of Commensal Lactic Acid Bacteria in Broiler Chickens

Meijerink

Oliveira

Haarlem

et al. 2021

Veterinary Sciences

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Restrictions on the use of antibiotics in the poultry industry stimulate the development of alternative nutritional solutions to maintain or improve poultry health. This requires more insight in the modulatory effects of feed additives on the immune system and microbiota composition. Compounds known to influence the innate immune system and microbiota composition were selected and screened in vitro, in ovo, and in vivo. Among all compounds, 57 enhanced NK cell activation, 56 increased phagocytosis, and 22 increased NO production of the macrophage cell line HD11 in vitro. Based on these results, availability and regulatory status, six compounds were selected for further analysis. None of these compounds showed negative effects on growth, hatchability, and feed conversion in in ovo and in vivo studies. Based on the most interesting numerical results and highest future potential feasibility, two compounds were analyzed further. Administration of glucose oligosaccharide and long-chain glucomannan in vivo both enhanced activation of intraepithelial NK cells and led to increased relative abundance of lactic acid bacteria (LAB) amongst ileum and ceca microbiota after seven days of supplementation. Positive correlations between NK cell subsets and activation, and relative abundance of LAB suggest the involvement of microbiota in the modulation of the function of intraepithelial NK cells. This study identifies glucose oligosaccharide and long-chain glucomannan supplementation as effective nutritional strategies to modulate the intestinal microbiota composition and strengthen the intraepithelial innate immune system.

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“…However, some studies reported that expression of some immune genes in placenta could be unexpected because of variable reasons, such as epigenetic modification and its unique microenvironment [ 18 , 19 ]. In addition, pregnant mice raised in conventional and germ-free environment represented different immunological adaptations [ 20 ]. These studies suggested that un identified immune modulation were occurred during pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of immune related genes between domestic pig and germ-free minipig

et al. 2020

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Recently, minipig has been considered as an animal model that is appropriate for human disease model to study toxicology, pharmacology, and xenotransplantation. Nevertheless, minipigs are bred in various environment according to their use. Here, we suggest that minipigs used for research should be bred in well-controlled facility, comparing immune status of pigs raised in different breeding environment. DNA microarray was performed with ear skin and placenta of Landrace domestic pigs (DPs) and Minnesota germ-free minipigs (GPs). Their immune transcriptome was analyzed by gene ontology (GO) annotation database, based on criteria of |log2 fold change| ≥1 with P ≤ 0.05. As a result, we found that immune related genes in the ear skin of DPs were highly activated, compared to GPs. On the other hand, no significant s were found in the placenta. Quantitative real-time PCR (qRT-PCR) was performed in five candidate immune genes. Their fold changes were consistent with the results from DNA microarray (P ≤ 0.05). In conclusion, we experimentally proved that porcine immune system was affected by different breeding environment, suggesting the importance of controlling microbes in animal room for the qualified research.

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Microbiota Induced Changes in the Immune Response in Pregnant Mice

Cited by 37 publications

References 48 publications

Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection

Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection

Glucose Oligosaccharide and Long-Chain Glucomannan Feed Additives Induce Enhanced Activation of Intraepithelial NK Cells and Relative Abundance of Commensal Lactic Acid Bacteria in Broiler Chickens

Comparative analysis of immune related genes between domestic pig and germ-free minipig

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