Microbiota-Mediated Competition Between<i>Drosophila</i>Species

Rombaut, Antoine; Gallet, Romain; Qitout, Kenza; Samy, Mukherjy; Guilhot, Robin; Ghirardini, Pauline; Lazzaro, Brian P.; Becher, Paul G.; Xuéreb, Anne; Gibert, Patricia; Fellous, Simon

doi:10.1101/2020.08.05.238055

Cited by 2 publications

(2 citation statements)

References 52 publications

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“…The microbiome plays a crucial role in influencing the ecological interactions among various Drosophila species, and has the potential to regulate insect populations (Meyling & Hajek, 2010; Rombaut et al., 2021). Many of these microbes are associated with the body surface, oral cavity, or digestive tract of the host species (Chandler et al., 2011) and may affect the host's biology, behaviour, and physiology (Elya et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

Host–pathogen interaction between the African fig fly, Zaprionus indianus, and its external mycobiome under laboratory conditions

Sharma,

Ramniwas,

Kumar

et al. 2024

Entomologia Exp Applicata

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The African fig fly, Zaprionus indianus Gupta (Diptera: Drosophilidae), is an ecologically diverse pest species that interacts with and feeds on various microbial pathogens including bacteria and yeast found in decomposing fruits. However, the interaction of Z. indianus with its microbial community and microbial pathogens is obscure. To determine the presence of fungal pathogens, we collected wild Z. indianus from the northwestern part of the Indian subcontinent. Based on molecular identification and phylogenetic analysis, Debaryomyces hansenii (Zopf) Lodder & Kreger‐van Rij (isolate ziha1) was the most commonly observed fungus associated with Z. indianus. Furthermore, we identified two more opportunistic fungal pathogens: Aspergillus flavus Link (isolate zias2), and Pichia kudriavzevii Boidin, Pignal & Besson (isolate zibd3). The interaction of isolated fungi with Z. indianus was evaluated in terms of larval mortality, adult emergence, and fecundity. Debaryomyces hansenii ziha1 resulted in 90% emergence rate for adults, and did not cause significant mortality in the larval stage. In contrast, A. flavus zias2 and P. kudriavzevii zibd3 showed a significant reduction in fecundity and caused 99% and 74% larval mortality of Z. indianus, respectively. In the laboratory oviposition preference assay, mated females of Z. indianus preferred D. hansenii ziha1 supplemented food with a positive oviposition index compared to the uninfected control and compared to food infected with P. kudriavzevii zibd3 or A. flavus zias2. These findings underscore Z. indianus' potential to act as a reservoir for both symbiotic and pathogenic fungal species, some of which may be further harnessed for effective fruit fly pest control strategies.

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Section: Discussionmentioning

confidence: 99%

Host–pathogen interaction between the African fig fly, Zaprionus indianus, and its external mycobiome under laboratory conditions

Sharma,

Ramniwas,

Kumar

et al. 2024

Entomologia Exp Applicata

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“…The fruit fly Drosophila melanogaster has been widely used as a genetically-tractable model to study host-microbe interactions, including microbiota resilience mechanisms [13][14][15][16][17][18][19]. Fruit flies rely on cellular and humoral arms of defence against invading pathogens [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

MprF-mediated immune evasion is necessary forLactiplantibacillus plantarumresilience inDrosophilagut during inflammation

Arias-Rojas,

Arifah,

Angelidou

et al. 2024

Preprint

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BackgroundMultiple peptide resistance factor (MprF) confers resistance to cationic antimicrobial peptides (AMPs) in several pathogens, thereby enabling evasion of the host immune response. While MprF has been proven to be crucial for the virulence of various pathogens, its role in commensal gut bacteria remains uncharacterized. To close this knowledge gap, we used a common gut commensal of animals,Lactiplantibacillus plantarum, and its natural host, the fruit flyDrosophila melanogaster, as an experimental model to investigate the role of MprF in commensal-host interactions.ResultsTheL. plantarumΔmprFmutant that we generated exhibited deficiency in the synthesis of lysyl-phosphatidylglycerol (Lys-PG), resulting in increased negative cell surface charge and increased susceptibility to AMPs. Susceptibility to AMPs had no effect on ΔmprFmutant’s ability to colonize guts of uninfected flies. However, we observed significantly reduced abundance of the ΔmprFmutant after infection-induced inflammation in the guts of wild-type flies but not flies lacking AMPs. These results demonstrate that host AMPs reduce the abundance of the ΔmprFmutant during infection. We found in addition that the ΔmprFmutant compared to wild-typeL. plantaruminduces a stronger intestinal immune response in flies due to the increased release of immunostimulatory peptidoglycan fragments, indicating an important role of MprF in promoting host tolerance to commensals.ConclusionOverall, our results demonstrate that MprF, besides its well-characterized role in pathogen immune evasion and virulence, is also an important resilience factor in maintaining stable microbiota-host interactions during intestinal inflammation.

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Microbiota-Mediated Competition BetweenDrosophilaSpecies

Cited by 2 publications

References 52 publications

Host–pathogen interaction between the African fig fly, Zaprionus indianus, and its external mycobiome under laboratory conditions

Host–pathogen interaction between the African fig fly, Zaprionus indianus, and its external mycobiome under laboratory conditions

MprF-mediated immune evasion is necessary forLactiplantibacillus plantarumresilience inDrosophilagut during inflammation

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